Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbA   Type   Machinery gene
Locus tag   RCF70_RS08850 Genome accession   NZ_CP133247
Coordinates   1793600..1794019 (-) Length   139 a.a.
NCBI ID   WP_105965184.1    Uniprot ID   A0AAE5SZ05
Organism   Staphylococcus chromogenes strain TA     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1760380..1803235 1793600..1794019 within 0


Gene organization within MGE regions


Location: 1760380..1803235
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RCF70_RS08650 (RCF70_08615) - 1760380..1761027 (-) 648 WP_107359669.1 ABC transporter ATP-binding protein -
  RCF70_RS08655 (RCF70_08620) - 1761039..1761596 (-) 558 WP_107359670.1 GNAT family N-acetyltransferase -
  RCF70_RS08660 (RCF70_08625) - 1762589..1763326 (-) 738 WP_037571949.1 hypothetical protein -
  RCF70_RS08665 (RCF70_08630) - 1763332..1764123 (-) 792 WP_037573026.1 HipA family kinase -
  RCF70_RS08670 (RCF70_08635) - 1764950..1766395 (-) 1446 WP_105985991.1 SH3 domain-containing protein -
  RCF70_RS08675 (RCF70_08640) - 1766376..1766810 (-) 435 WP_105967198.1 phage holin -
  RCF70_RS08680 (RCF70_08645) - 1766931..1767224 (-) 294 WP_037571969.1 DUF2951 family protein -
  RCF70_RS08685 (RCF70_08650) - 1767240..1767536 (-) 297 WP_105965207.1 hypothetical protein -
  RCF70_RS08690 (RCF70_08655) - 1767583..1767723 (-) 141 WP_182470811.1 hypothetical protein -
  RCF70_RS08695 (RCF70_08660) - 1767707..1771180 (-) 3474 WP_419791656.1 phage tail spike protein -
  RCF70_RS08700 (RCF70_08665) - 1771182..1772672 (-) 1491 WP_107364338.1 phage distal tail protein -
  RCF70_RS08705 (RCF70_08670) - 1772672..1777249 (-) 4578 WP_107364339.1 phage tail tape measure protein -
  RCF70_RS08710 (RCF70_08675) gpG 1777275..1777907 (-) 633 WP_419791657.1 phage tail assembly chaperone G -
  RCF70_RS08715 (RCF70_08680) - 1777977..1778915 (-) 939 WP_105963560.1 major tail protein -
  RCF70_RS08720 (RCF70_08685) - 1778927..1779289 (-) 363 WP_107360637.1 DUF806 family protein -
  RCF70_RS08725 (RCF70_08690) - 1779286..1779663 (-) 378 WP_107360638.1 HK97-gp10 family putative phage morphogenesis protein -
  RCF70_RS08730 (RCF70_08695) - 1779663..1779995 (-) 333 WP_105963557.1 head-tail adaptor protein -
  RCF70_RS08735 (RCF70_08700) - 1779976..1780266 (-) 291 WP_107360639.1 head-tail connector protein -
  RCF70_RS08740 (RCF70_08705) - 1780276..1780434 (-) 159 WP_164671795.1 hypothetical protein -
  RCF70_RS08745 (RCF70_08710) - 1780447..1781694 (-) 1248 WP_107360640.1 phage major capsid protein -
  RCF70_RS08750 (RCF70_08715) - 1781763..1782362 (-) 600 WP_419791658.1 HK97 family phage prohead protease -
  RCF70_RS08755 (RCF70_08720) - 1782346..1783611 (-) 1266 WP_198645131.1 phage portal protein -
  RCF70_RS08760 (RCF70_08725) - 1783827..1785530 (-) 1704 WP_107360642.1 terminase large subunit -
  RCF70_RS08765 (RCF70_08730) - 1785530..1786000 (-) 471 WP_107360643.1 phage terminase small subunit P27 family -
  RCF70_RS08770 (RCF70_08735) - 1786082..1786459 (-) 378 WP_107360644.1 HNH endonuclease -
  RCF70_RS08775 (RCF70_08740) - 1786464..1786826 (-) 363 WP_105965195.1 hypothetical protein -
  RCF70_RS08780 (RCF70_08745) - 1787009..1787404 (-) 396 WP_103158818.1 hypothetical protein -
  RCF70_RS08785 (RCF70_08750) - 1787415..1787570 (-) 156 WP_157946201.1 hypothetical protein -
  RCF70_RS08790 (RCF70_08755) - 1787670..1787876 (-) 207 WP_107360645.1 hypothetical protein -
  RCF70_RS08795 (RCF70_08760) - 1787924..1788430 (-) 507 WP_107360646.1 dUTP diphosphatase -
  RCF70_RS08800 (RCF70_08765) - 1788540..1788854 (+) 315 WP_107360647.1 DUF4870 domain-containing protein -
  RCF70_RS08805 (RCF70_08770) - 1788882..1789094 (-) 213 WP_105965190.1 hypothetical protein -
  RCF70_RS08810 (RCF70_08775) - 1789094..1789510 (-) 417 WP_234014784.1 DUF3310 domain-containing protein -
  RCF70_RS08815 (RCF70_08780) - 1789507..1789929 (-) 423 WP_105965189.1 hypothetical protein -
  RCF70_RS08820 (RCF70_08785) - 1789931..1790353 (-) 423 WP_060550724.1 RusA family crossover junction endodeoxyribonuclease -
  RCF70_RS08825 (RCF70_08790) - 1790340..1790558 (-) 219 WP_105965188.1 hypothetical protein -
  RCF70_RS08830 (RCF70_08795) - 1790555..1791793 (-) 1239 WP_105965187.1 DnaB helicase C-terminal domain-containing protein -
  RCF70_RS08835 (RCF70_08800) - 1791786..1792127 (-) 342 WP_234014782.1 hypothetical protein -
  RCF70_RS08840 (RCF70_08805) - 1792124..1792918 (-) 795 WP_105965186.1 phage replisome organizer N-terminal domain-containing protein -
  RCF70_RS08845 (RCF70_08810) - 1792911..1793588 (-) 678 WP_105965185.1 putative HNHc nuclease -
  RCF70_RS08850 (RCF70_08815) ssbA 1793600..1794019 (-) 420 WP_105965184.1 single-stranded DNA-binding protein Machinery gene
  RCF70_RS08855 (RCF70_08820) - 1794019..1794642 (-) 624 WP_107364341.1 ERF family protein -
  RCF70_RS08860 (RCF70_08825) - 1794642..1795127 (-) 486 WP_105967181.1 siphovirus Gp157 family protein -
  RCF70_RS08865 (RCF70_08830) - 1795124..1795390 (-) 267 WP_105965181.1 DUF1108 family protein -
  RCF70_RS08870 (RCF70_08835) - 1795642..1795854 (-) 213 WP_105965180.1 hypothetical protein -
  RCF70_RS08875 (RCF70_08840) - 1796237..1796497 (-) 261 WP_107364342.1 hypothetical protein -
  RCF70_RS08880 (RCF70_08845) - 1796581..1796961 (+) 381 WP_105963625.1 DUF2513 domain-containing protein -
  RCF70_RS08885 (RCF70_08850) - 1796948..1797145 (-) 198 WP_037572072.1 hypothetical protein -
  RCF70_RS08890 (RCF70_08855) tscA 1797182..1797406 (-) 225 WP_105965178.1 type II toxin-antitoxin system antitoxin TscA -
  RCF70_RS08895 (RCF70_08860) - 1797418..1798227 (-) 810 WP_234014781.1 Bro-N domain-containing protein -
  RCF70_RS08900 (RCF70_08865) - 1798241..1798462 (-) 222 WP_105965177.1 transcriptional regulator -
  RCF70_RS08905 (RCF70_08870) - 1798658..1799350 (+) 693 WP_105965176.1 helix-turn-helix domain-containing protein -
  RCF70_RS08910 (RCF70_08875) - 1799367..1799528 (+) 162 WP_157947099.1 hypothetical protein -
  RCF70_RS08915 (RCF70_08880) - 1799570..1800091 (+) 522 WP_107364343.1 hypothetical protein -
  RCF70_RS08920 (RCF70_08885) - 1800593..1800769 (+) 177 WP_157946230.1 hypothetical protein -
  RCF70_RS08925 (RCF70_08890) - 1801035..1802003 (+) 969 WP_037572091.1 DUF3644 domain-containing protein -
  RCF70_RS08930 (RCF70_08895) - 1802186..1803235 (+) 1050 WP_105962989.1 tyrosine-type recombinase/integrase -

Sequence


Protein


Download         Length: 139 a.a.        Molecular weight: 15652.23 Da        Isoelectric Point: 4.8892

>NTDB_id=872794 RCF70_RS08850 WP_105965184.1 1793600..1794019(-) (ssbA) [Staphylococcus chromogenes strain TA]
MLNRVVLVGRLTKDPEFRTTPSGVDVSTFTLAVNRNFKNKDGEQQADFINCVVFRKQAENVKNFLSKGSLAGVDGRMQSR
SYENKEGQRVYVTEVVCDSVQFLDPKNNNQQNNQQQNGQTQTGNNPFDNASIDDDDLPF

Nucleotide


Download         Length: 420 bp        

>NTDB_id=872794 RCF70_RS08850 WP_105965184.1 1793600..1794019(-) (ssbA) [Staphylococcus chromogenes strain TA]
ATGCTTAACAGAGTTGTATTAGTAGGACGATTAACAAAAGACCCTGAATTCAGAACGACGCCATCAGGCGTAGACGTATC
AACATTCACACTTGCGGTAAATCGCAATTTCAAAAATAAAGATGGAGAACAACAAGCTGACTTTATTAATTGCGTTGTAT
TCCGCAAGCAAGCTGAAAACGTCAAAAACTTTTTAAGTAAAGGTAGCTTAGCAGGCGTTGATGGACGGATGCAATCACGC
AGTTACGAAAACAAAGAAGGTCAACGTGTTTATGTAACTGAAGTCGTTTGTGACAGTGTTCAATTTCTAGACCCAAAGAA
TAACAACCAACAGAATAACCAACAACAAAACGGACAAACACAAACAGGTAATAATCCTTTTGATAACGCTAGTATCGATG
ACGACGATTTACCTTTCTAG


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

55.233

100

0.683

  ssb Latilactobacillus sakei subsp. sakei 23K

51.176

100

0.626

  ssbB Bacillus subtilis subsp. subtilis str. 168

60.377

76.259

0.46

  ssbB Streptococcus sobrinus strain NIDR 6715-7

42.446

100

0.424

  ssbA Streptococcus mutans UA159

40.288

100

0.403

  ssbB/cilA Streptococcus mitis NCTC 12261

37.41

100

0.374

  ssbB/cilA Streptococcus pneumoniae TIGR4

37.41

100

0.374

  ssbB/cilA Streptococcus pneumoniae Rx1

36.691

100

0.367

  ssbB/cilA Streptococcus pneumoniae D39

36.691

100

0.367

  ssbB/cilA Streptococcus pneumoniae R6

36.691

100

0.367

  ssbB/cilA Streptococcus mitis SK321

36.691

100

0.367