Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   Q7609_RS04020 Genome accession   NZ_CP131725
Coordinates   778985..779425 (+) Length   146 a.a.
NCBI ID   WP_342123400.1    Uniprot ID   -
Organism   Haemophilus influenzae strain 2018S05-172     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 773985..784425
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q7609_RS04000 (Q7609_04000) rsmE 774445..775182 (-) 738 WP_005654099.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  Q7609_RS04005 (Q7609_04005) lnt 775232..776761 (-) 1530 WP_151230905.1 apolipoprotein N-acyltransferase -
  Q7609_RS04010 (Q7609_04010) corC 776784..777683 (-) 900 WP_005654105.1 CNNM family magnesium/cobalt transport protein CorC -
  Q7609_RS04015 (Q7609_04015) ampD 778316..778870 (-) 555 WP_342123399.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  Q7609_RS04020 (Q7609_04020) pilA 778985..779425 (+) 441 WP_342123400.1 prepilin peptidase-dependent pilin Machinery gene
  Q7609_RS04025 (Q7609_04025) pilB 779422..780813 (+) 1392 WP_342123401.1 GspE/PulE family protein Machinery gene
  Q7609_RS04030 (Q7609_04030) pilC 780810..782030 (+) 1221 WP_342123402.1 type II secretion system F family protein Machinery gene
  Q7609_RS04035 (Q7609_04035) pilD 782027..782719 (+) 693 WP_342123403.1 A24 family peptidase Machinery gene
  Q7609_RS04040 (Q7609_04040) rho 782773..784035 (-) 1263 WP_005648966.1 transcription termination factor Rho -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15146.37 Da        Isoelectric Point: 8.4623

>NTDB_id=864870 Q7609_RS04020 WP_342123400.1 778985..779425(+) (pilA) [Haemophilus influenzae strain 2018S05-172]
MKLTTQQTLKKGFTLIELMIVIAIIAILATIAIPSYQNYTKKAAVSELLQASAPYKSDVELCVYSTGTPSTCSGGRNGIA
ADITTAKGYVKSVTTNNGAITVAGNGTLDGMSYTLTAEGDSAKGVTWKTTCGTTNADIFPAGFCSK

Nucleotide


Download         Length: 441 bp        

>NTDB_id=864870 Q7609_RS04020 WP_342123400.1 778985..779425(+) (pilA) [Haemophilus influenzae strain 2018S05-172]
ATGAAATTAACAACACAGCAAACCTTGAAAAAAGGTTTTACATTAATAGAGCTAATGATTGTGATTGCAATTATTGCTAT
TTTAGCCACCATCGCAATTCCTTCTTATCAAAATTATACCAAAAAAGCTGCGGTATCCGAATTACTGCAAGCTTCTGCGC
CTTATAAGTCAGATGTGGAATTATGCGTTTATAGCACAGGCACACCTTCAACTTGCTCAGGAGGAAGAAATGGAATTGCT
GCTGATATTACAACAGCAAAAGGCTATGTAAAATCAGTGACAACAAACAACGGTGCAATAACAGTAGCGGGTAATGGTAC
ATTAGATGGAATGAGTTACACGCTCACGGCTGAAGGCGATAGTGCAAAAGGCGTTACATGGAAAACCACCTGTGGCACAA
CGAATGCCGATATTTTCCCTGCTGGATTCTGTTCAAAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)

Pilin (bacterial filament) (Pilin)

(42-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

81.25

98.63

0.801
  pilA Haemophilus influenzae Rd KW20

77.778

98.63

0.767
  pilA Glaesserella parasuis strain SC1401

55.405

100

0.562
  pilA2 Legionella pneumophila strain ERS1305867

42.177

100

0.425
  pilA2 Legionella pneumophila str. Paris

42.177

100

0.425
  comP Acinetobacter baylyi ADP1

41.379

99.315

0.411
  pilA Vibrio campbellii strain DS40M4

36.129

100

0.384
  pilA/pilAI Pseudomonas stutzeri DSM 10701

37.762

97.945

0.37