Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   Q7609_RS04025 Genome accession   NZ_CP131725
Coordinates   779422..780813 (+) Length   463 a.a.
NCBI ID   WP_342123401.1    Uniprot ID   -
Organism   Haemophilus influenzae strain 2018S05-172     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 774422..785813
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q7609_RS04000 (Q7609_04000) rsmE 774445..775182 (-) 738 WP_005654099.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  Q7609_RS04005 (Q7609_04005) lnt 775232..776761 (-) 1530 WP_151230905.1 apolipoprotein N-acyltransferase -
  Q7609_RS04010 (Q7609_04010) corC 776784..777683 (-) 900 WP_005654105.1 CNNM family magnesium/cobalt transport protein CorC -
  Q7609_RS04015 (Q7609_04015) ampD 778316..778870 (-) 555 WP_342123399.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  Q7609_RS04020 (Q7609_04020) pilA 778985..779425 (+) 441 WP_342123400.1 prepilin peptidase-dependent pilin Machinery gene
  Q7609_RS04025 (Q7609_04025) pilB 779422..780813 (+) 1392 WP_342123401.1 GspE/PulE family protein Machinery gene
  Q7609_RS04030 (Q7609_04030) pilC 780810..782030 (+) 1221 WP_342123402.1 type II secretion system F family protein Machinery gene
  Q7609_RS04035 (Q7609_04035) pilD 782027..782719 (+) 693 WP_342123403.1 A24 family peptidase Machinery gene
  Q7609_RS04040 (Q7609_04040) rho 782773..784035 (-) 1263 WP_005648966.1 transcription termination factor Rho -
  Q7609_RS04045 (Q7609_04045) metJ 784283..784600 (+) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  Q7609_RS04050 (Q7609_04050) cueR 784614..785000 (-) 387 WP_005654127.1 Cu(I)-responsive transcriptional regulator -
  Q7609_RS04055 (Q7609_04055) - 785077..785283 (+) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -
  Q7609_RS04060 (Q7609_04060) - 785369..785575 (+) 207 WP_005666693.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 463 a.a.        Molecular weight: 52749.40 Da        Isoelectric Point: 6.2490

>NTDB_id=864871 Q7609_RS04025 WP_342123401.1 779422..780813(+) (pilB) [Haemophilus influenzae strain 2018S05-172]
MIDLTSAKPRVKAQNGEVFTILPDLWARNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKPVEPILL
ESSQLKELLQQLTPCQMQVEEQVKFYQHQETHFEQENDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVLQ
PQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFSELGM
TENQQQAFQRALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFNRLLRT
FLRQDPDIIMLGEIRDEESAMIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQYEIKNSLLLVIAQRLVRKLCSKCG
GNLANSCDCHQGYRGRIGVYQFLHWQQNDYQTDFKNLRASGLEKVSQGITDEKEIERVLGKNL

Nucleotide


Download         Length: 1392 bp        

>NTDB_id=864871 Q7609_RS04025 WP_342123401.1 779422..780813(+) (pilB) [Haemophilus influenzae strain 2018S05-172]
ATGATCGACTTAACTAGTGCAAAACCTCGTGTAAAAGCACAAAATGGCGAAGTATTTACGATCTTGCCTGATTTATGGGC
GCGTAATCAACAACAGCAATCCTTGCTCTTGCGGTATTTTGCTTTGCCACTTAAAGAAGAAAATAATCGTCTTTGGCTAG
GGGTTGATTCTCTCTCCAATCTTTCAGCTTGTGAAACCATTGCGTTTATAACGGGAAAACCTGTCGAACCAATTTTGTTA
GAAAGCAGCCAACTCAAAGAACTGTTACAACAACTTACTCCGTGCCAAATGCAAGTGGAAGAACAAGTTAAATTCTATCA
ACATCAAGAAACCCATTTTGAACAAGAAAATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCTGCCTTAC
AAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAGTTTCAAGTGCGGTTTAGAATTGATGGTGTTTTACAA
CCACAACCCTTAATAAGCAAAATATTCGCCAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATATTAGTGA
AAATCGACTTCCACAAGATGGGCGGTTTCAATTTAAAACGACTTTTTCCGATATTCTTGATTTTCGCCTTTCAACCTTAC
CAACCCATTGGGGCGAAAAAATCGTGTTGCGAGCGCAACAAAATAAACCAGTAGAACTTAGCTTTTCTGAATTAGGTATG
ACAGAAAATCAGCAACAAGCATTTCAACGCGCACTTAGCCAGCCACAAGGATTAATTTTAGTCACTGGTCCAACAGGAAG
TGGAAAAAGTATCTCACTTTACACCGCACTTCAGTGGCTAAATACGCCTGATAAACATATTATGACTGCTGAAGATCCCA
TTGAAATTGAGCTGGATGGCATTATTCAAAGCCAAATTAATCCACAGATTGGATTAGATTTTAACCGCCTATTACGCACT
TTTTTACGTCAAGATCCCGATATTATTATGCTAGGCGAAATTCGCGATGAAGAAAGTGCAATGATTGCATTACGTGCGGC
ACAAACTGGGCATTTGGTGCTTTCAACTTTACATACCAATGATGCAATTTCTGCTATTTCTCGCTTACAACAACTCGGTA
TTCAACAGTATGAAATTAAAAACAGTTTATTGCTCGTTATTGCACAGCGTCTTGTACGAAAGCTTTGTTCAAAGTGCGGT
GGAAATTTAGCAAATTCTTGTGATTGCCATCAAGGTTATCGAGGTCGAATCGGCGTGTATCAATTTCTACATTGGCAGCA
GAATGACTATCAAACGGATTTTAAAAATTTACGTGCGAGTGGTTTAGAAAAAGTTAGCCAAGGCATAACGGATGAGAAAG
AAATTGAACGTGTGTTAGGTAAAAATTTATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(31-94)

Type II/IV secretion system protein (T2SSE)

(127-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae Rd KW20

98.458

98.056

0.965
  pilB Haemophilus influenzae 86-028NP

95.806

97.84

0.937
  pilB Glaesserella parasuis strain SC1401

58.17

99.136

0.577
  pilB Vibrio cholerae strain A1552

40.702

100

0.425
  pilB Acinetobacter baylyi ADP1

39.315

100

0.421
  pilB Vibrio campbellii strain DS40M4

40.206

100

0.421
  pilB Vibrio parahaemolyticus RIMD 2210633

40.166

100

0.419
  pilB Acinetobacter baumannii D1279779

39.588

100

0.415
  pilB Legionella pneumophila strain ERS1305867

38.855

100

0.41
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.401

100

0.384
  pilF Neisseria gonorrhoeae MS11

36.975

100

0.38
  pilF Thermus thermophilus HB27

36.214

100

0.38