Detailed information NTG0856032

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: V9K85_RS04350
• Genome accession: NZ_CP146650
• Coordinates: 882975..883955 (+)
• Length: 326 a.a.
• NCBI ID: WP_001324408.1
• Uniprot ID: -
• Organism: Escherichia coli strain MS 85-1
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 877975..888955

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V9K85_RS04320 (V9K85_04320)	yggM	878490..879497 (+)	1008	WP_000745220.1	DUF1202 family protein	-
V9K85_RS04325 (V9K85_04325)	hemW	879652..880788 (-)	1137	WP_000239928.1	radical SAM family heme chaperone HemW	-
V9K85_RS04330 (V9K85_04330)	rdgB	880781..881374 (-)	594	WP_001174743.1	XTP/dITP diphosphatase	-
V9K85_RS04335 (V9K85_04335)	yggU	881382..881672 (-)	291	WP_001277222.1	DUF167 family protein YggU	-
V9K85_RS04340 (V9K85_04340)	yggT	881669..882235 (-)	567	WP_001094831.1	osmotic shock tolerance protein YggT	-
V9K85_RS04345 (V9K85_04345)	yggS	882253..882957 (-)	705	WP_000997795.1	pyridoxal phosphate homeostasis protein	-
V9K85_RS04350 (V9K85_04350)	pilT	882975..883955 (+)	981	WP_001324408.1	type IV pilus twitching motility protein PilT	Machinery gene
V9K85_RS04355 (V9K85_04355)	ruvX	884118..884534 (-)	417	WP_000017106.1	Holliday junction resolvase RuvX	-
V9K85_RS04360 (V9K85_04360)	yqgE	884534..885097 (-)	564	WP_001053178.1	YqgE/AlgH family protein	-
V9K85_RS04365 (V9K85_04365)	gshB	885206..886156 (-)	951	WP_000593273.1	glutathione synthase	-
V9K85_RS04370 (V9K85_04370)	rsmE	886169..886900 (-)	732	WP_001222508.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
V9K85_RS04375 (V9K85_04375)	endA	886980..887687 (-)	708	WP_000286500.1	deoxyribonuclease I	-
V9K85_RS04380 (V9K85_04380)	yggI	887782..888279 (-)	498	WP_000858396.1	SprT family zinc-dependent metalloprotease	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 35873.07 Da
• Isoelectric Point: 5.5857

>NTDB_id=856032 V9K85_RS04350 WP_001324408.1 882975..883955(+) (pilT) [Escherichia coli strain MS 85-1]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGCVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSFQQR
VGEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=856032 V9K85_RS04350 WP_001324408.1 882975..883955(+) (pilT) [Escherichia coli strain MS 85-1]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGTGGCAGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTTAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACTTTCGCATCGGGGTTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTACGTGACAGTGAGACAATCCGTCTGGCGCTAACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCAACCTTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGATGCGTGGCGCTGTTTGAATTATTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTTTCAGCAGCGA
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (62-239)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae strain A1552	49.235	100	0.494
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.235	100	0.494
pilT	Neisseria meningitidis 8013	48.476	100	0.488
pilT	Neisseria gonorrhoeae MS11	48.171	100	0.485
pilT	Acinetobacter baylyi ADP1	46.483	100	0.466
pilT	Acinetobacter baumannii D1279779	46.177	100	0.463
pilT	Acinetobacter nosocomialis M2	46.177	100	0.463
pilT	Acinetobacter baumannii strain A118	46.177	100	0.463
pilT	Pseudomonas stutzeri DSM 10701	46.177	100	0.463
pilT	Pseudomonas aeruginosa PAK	45.872	100	0.46
pilT	Legionella pneumophila strain ERS1305867	44.954	100	0.451
pilT	Legionella pneumophila strain Lp02	44.954	100	0.451
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.824	97.546	0.408
pilU	Vibrio cholerae strain A1552	39.21	100	0.396
pilU	Pseudomonas stutzeri DSM 10701	37.273	100	0.377
pilB	Legionella pneumophila strain ERS1305867	30.89	100	0.362