Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbB   Type   Machinery gene
Locus tag   QYH60_RS00565 Genome accession   NZ_CP129292
Coordinates   102252..102641 (-) Length   129 a.a.
NCBI ID   WP_010905314.1    Uniprot ID   S6EQX8
Organism   Lactococcus lactis subsp. lactis strain KMGR2-43     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 83247..102641 102252..102641 within 0


Gene organization within MGE regions


Location: 83247..102641
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QYH60_RS00430 (QYH60_00430) - 83247..84446 (-) 1200 WP_301401194.1 site-specific integrase -
  QYH60_RS00435 (QYH60_00435) - 84513..85043 (-) 531 WP_023163772.1 helix-turn-helix transcriptional regulator -
  QYH60_RS00440 (QYH60_00440) - 85190..85384 (+) 195 WP_023163771.1 transcriptional regulator -
  QYH60_RS00445 (QYH60_00445) - 85422..85685 (+) 264 WP_212656564.1 hypothetical protein -
  QYH60_RS00450 (QYH60_00450) - 85813..86007 (+) 195 WP_023163769.1 DUF1655 domain-containing protein -
  QYH60_RS00455 (QYH60_00455) - 86012..86530 (+) 519 WP_301401195.1 hypothetical protein -
  QYH60_RS00460 (QYH60_00460) - 86527..86796 (+) 270 WP_163656914.1 hypothetical protein -
  QYH60_RS00465 (QYH60_00465) - 87060..87386 (+) 327 WP_301401196.1 HTH domain-containing protein -
  QYH60_RS00470 (QYH60_00470) - 87395..88237 (+) 843 WP_301401197.1 phage replisome organiser protein -
  QYH60_RS00475 (QYH60_00475) - 88249..89142 (+) 894 WP_153951468.1 ATP-binding protein -
  QYH60_RS00480 (QYH60_00480) - 89153..89302 (+) 150 WP_153951467.1 hypothetical protein -
  QYH60_RS00485 (QYH60_00485) - 89421..89735 (+) 315 WP_301401198.1 hypothetical protein -
  QYH60_RS00490 (QYH60_00490) - 89722..90321 (+) 600 WP_301401199.1 hypothetical protein -
  QYH60_RS00495 (QYH60_00495) - 90529..90840 (+) 312 WP_301401200.1 hypothetical protein -
  QYH60_RS00500 (QYH60_00500) - 90840..91172 (+) 333 WP_081199223.1 hypothetical protein -
  QYH60_RS00505 (QYH60_00505) - 91851..92588 (+) 738 WP_301401201.1 hypothetical protein -
  QYH60_RS00510 (QYH60_00510) - 92866..93057 (+) 192 WP_012897234.1 hypothetical protein -
  QYH60_RS00515 (QYH60_00515) rsmI 93134..94009 (-) 876 WP_301401202.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  QYH60_RS00520 (QYH60_00520) yabA 94009..94335 (-) 327 WP_011675472.1 DNA replication initiation control protein YabA -
  QYH60_RS00525 (QYH60_00525) - 94328..95110 (-) 783 WP_301401203.1 stage 0 sporulation family protein -
  QYH60_RS00530 (QYH60_00530) - 95162..96022 (-) 861 WP_012897231.1 DNA polymerase III subunit delta' -
  QYH60_RS00535 (QYH60_00535) tmk 96155..96790 (-) 636 WP_012897230.1 dTMP kinase -
  QYH60_RS00540 (QYH60_00540) yycF 96968..97669 (+) 702 WP_003131580.1 response regulator YycF -
  QYH60_RS00545 (QYH60_00545) - 97662..99104 (+) 1443 WP_301401204.1 cell wall metabolism sensor histidine kinase WalK -
  QYH60_RS00550 (QYH60_00550) - 99273..100085 (+) 813 WP_003131583.1 MBL fold metallo-hydrolase -
  QYH60_RS00555 (QYH60_00555) groL 100131..101759 (-) 1629 WP_003131585.1 chaperonin GroEL -
  QYH60_RS00560 (QYH60_00560) groES 101848..102132 (-) 285 WP_003131589.1 co-chaperone GroES -
  QYH60_RS00565 (QYH60_00565) ssbB 102252..102641 (-) 390 WP_010905314.1 single-stranded DNA-binding protein Machinery gene

Sequence


Protein


Download         Length: 129 a.a.        Molecular weight: 14672.73 Da        Isoelectric Point: 8.3705

>NTDB_id=852067 QYH60_RS00565 WP_010905314.1 102252..102641(-) (ssbB) [Lactococcus lactis subsp. lactis strain KMGR2-43]
MNKTMLIGRLTSAPEISKTTNDKSYVRVTLAVNRRFKNEKGEREADFISIIIWGKSAETLVSYAKKGSLISIEGEIRTRN
YTDKQNQKHYVTEILGLSYDLLESRATIALRESAIKTEETLLDAEELPF

Nucleotide


Download         Length: 390 bp        

>NTDB_id=852067 QYH60_RS00565 WP_010905314.1 102252..102641(-) (ssbB) [Lactococcus lactis subsp. lactis strain KMGR2-43]
ATGAATAAAACCATGTTAATTGGACGCTTAACCAGTGCGCCAGAAATTTCAAAAACAACAAATGATAAATCATATGTCCG
TGTGACCTTAGCAGTCAATCGCCGTTTCAAAAATGAAAAAGGAGAACGAGAGGCAGATTTTATTTCAATTATTATTTGGG
GAAAATCTGCAGAAACTTTAGTTTCTTACGCAAAAAAAGGAAGTCTTATTTCAATTGAAGGAGAAATTAGAACTAGAAAT
TATACGGATAAGCAAAATCAAAAACATTATGTCACAGAAATCTTAGGACTGAGTTATGATTTACTTGAAAGTAGGGCAAC
AATCGCTTTACGAGAAAGCGCTATAAAGACTGAAGAAACCTTACTTGATGCGGAAGAACTCCCTTTCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB S6EQX8

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbB Lactococcus lactis subsp. cremoris KW2

90.698

100

0.907

  ssbB Streptococcus sobrinus strain NIDR 6715-7

60.769

100

0.612

  ssbB/cilA Streptococcus pneumoniae TIGR4

56.923

100

0.574

  ssbB/cilA Streptococcus pneumoniae Rx1

56.154

100

0.566

  ssbB/cilA Streptococcus pneumoniae D39

56.154

100

0.566

  ssbB/cilA Streptococcus pneumoniae R6

56.154

100

0.566

  ssbB/cilA Streptococcus mitis NCTC 12261

56.154

100

0.566

  ssbB/cilA Streptococcus mitis SK321

56.154

100

0.566

  ssbA Streptococcus mutans UA159

55.385

100

0.558

  ssb Latilactobacillus sakei subsp. sakei 23K

43.75

86.822

0.38

  ssbA Bacillus subtilis subsp. subtilis str. 168

44.762

81.395

0.364