Detailed information NTG0851332

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: LLUC029_RS03565
• Genome accession: NZ_CP129163
• Coordinates: 692588..693058 (+)
• Length: 156 a.a.
• NCBI ID: WP_231099719.1
• Uniprot ID: -
• Organism: Lactococcus cremoris strain UC029
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 687588..698058

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLUC029_RS03545 (LLUC029_03550)	-	688874..689434 (+)	561	WP_032950467.1	GNAT family N-acetyltransferase	-
LLUC029_RS03550 (LLUC029_03555)	-	689551..689949 (+)	399	WP_011676775.1	hypothetical protein	-
LLUC029_RS03555 (LLUC029_03560)	-	690055..690987 (+)	933	WP_032950468.1	ABC transporter ATP-binding protein	-
LLUC029_RS03560 (LLUC029_03565)	-	690984..692345 (+)	1362	WP_032950469.1	ABC transporter permease	-
LLUC029_RS03565 (LLUC029_03570)	comEA	692588..693058 (+)	471	WP_231099719.1	helix-hairpin-helix domain-containing protein	Machinery gene
LLUC029_RS03570 (LLUC029_03575)	comEC	693186..694457 (+)	1272	WP_324187043.1	ComEC/Rec2 family competence protein	Machinery gene
LLUC029_RS03575 (LLUC029_03580)	comEC	694454..694963 (+)	510	WP_021165649.1	MBL fold metallo-hydrolase	Machinery gene
LLUC029_RS03580 (LLUC029_03585)	-	695040..695930 (+)	891	WP_015082326.1	IS982-like element IS982B family transposase	-
LLUC029_RS03585 (LLUC029_03590)	-	695951..696256 (+)	306	Protein_697	ComEC/Rec2 family competence protein	-
LLUC029_RS03590 (LLUC029_03595)	-	696540..697316 (+)	777	WP_014572211.1	alpha/beta hydrolase family protein	-
LLUC029_RS03595 (LLUC029_03600)	-	697499..697714 (+)	216	WP_014572212.1	F0F1 ATP synthase subunit C	-

Sequence

Protein

• Length: 156 a.a.
• Molecular weight: 17024.20 Da
• Isoelectric Point: 5.0572

>NTDB_id=851332 LLUC029_RS03565 WP_231099719.1 692588..693058(+) (comEA) [Lactococcus cremoris strain UC029]
MKKNKNEIMVDLKGAVAKPNVYQISSDERLVDLIRQAGGFTDQADQKSINLSAKLKDEEVIYVPKLGESSSSESTDSPIS
SSVSNQVSTTSGPKININKADLTELQKLTGIGQKKAQDIIDFRMKNGDFKSIEDLGKVSGFGDKTLEKLKDEISID

Nucleotide

• Length: 471 bp

>NTDB_id=851332 LLUC029_RS03565 WP_231099719.1 692588..693058(+) (comEA) [Lactococcus cremoris strain UC029]
GTGAAAAAAAATAAAAACGAAATTATGGTTGATTTGAAAGGAGCGGTTGCAAAACCTAATGTATACCAAATTTCGTCAGA
TGAGCGCCTTGTTGATTTAATTAGGCAAGCGGGAGGATTTACTGACCAAGCAGACCAAAAATCAATCAATCTGTCAGCGA
AACTTAAAGATGAAGAAGTGATTTATGTACCAAAACTTGGGGAAAGTTCAAGTTCAGAAAGTACTGACAGTCCTATTAGC
AGCTCTGTCAGTAATCAAGTTTCAACGACAAGTGGTCCAAAAATAAATATCAATAAAGCAGACCTGACAGAATTACAAAA
ACTGACTGGAATTGGTCAAAAAAAAGCGCAAGATATTATTGATTTTCGCATGAAAAATGGTGACTTTAAATCAATAGAGG
ATTTGGGTAAAGTATCTGGCTTTGGGGATAAAACATTAGAAAAATTGAAAGATGAGATTTCTATTGATTAA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (92-153)

• SLBB domain (SLBB) (9-64)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	96.129	99.359	0.955
comEA/celA/cilE	Streptococcus mitis SK321	51.007	95.513	0.487
comEA	Latilactobacillus sakei subsp. sakei 23K	44.512	100	0.468
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	48.993	95.513	0.468
comEA/celA/cilE	Streptococcus pneumoniae Rx1	48.322	95.513	0.462
comEA/celA/cilE	Streptococcus pneumoniae D39	48.322	95.513	0.462
comEA/celA/cilE	Streptococcus pneumoniae R6	48.322	95.513	0.462
comEA/celA/cilE	Streptococcus mitis NCTC 12261	47.651	95.513	0.455
comEA	Streptococcus thermophilus LMD-9	42.5	100	0.436
comEA	Staphylococcus aureus N315	41.83	98.077	0.41
comEA	Staphylococcus aureus MW2	41.83	98.077	0.41
comEA	Bacillus subtilis subsp. subtilis str. 168	41.216	94.872	0.391