Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QVG61_RS10540 Genome accession   NZ_CP128988
Coordinates   2285578..2287293 (+) Length   571 a.a.
NCBI ID   WP_289930597.1    Uniprot ID   -
Organism   Thiohalobacter sp. IOR34     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2280578..2292293
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QVG61_RS10520 (QVG61_10520) - 2281186..2281419 (+) 234 WP_289930593.1 PP0621 family protein -
  QVG61_RS10525 (QVG61_10525) - 2281427..2283091 (+) 1665 WP_289930594.1 ATP-binding protein -
  QVG61_RS10530 (QVG61_10530) pilR 2283088..2284452 (+) 1365 WP_289930595.1 sigma-54 dependent transcriptional regulator Regulator
  QVG61_RS10535 (QVG61_10535) pilA 2284872..2285303 (+) 432 WP_289930596.1 pilin Machinery gene
  QVG61_RS10540 (QVG61_10540) pilB 2285578..2287293 (+) 1716 WP_289930597.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QVG61_RS10545 (QVG61_10545) pilC 2287300..2288520 (+) 1221 WP_289930598.1 type II secretion system F family protein Machinery gene
  QVG61_RS10550 (QVG61_10550) pilD 2288558..2289433 (+) 876 WP_289930599.1 A24 family peptidase Machinery gene
  QVG61_RS10555 (QVG61_10555) coaE 2289470..2290087 (+) 618 WP_289930600.1 dephospho-CoA kinase -
  QVG61_RS10560 (QVG61_10560) zapD 2290574..2291344 (+) 771 WP_289930601.1 cell division protein ZapD -
  QVG61_RS10565 (QVG61_10565) yacG 2291702..2291899 (+) 198 WP_289930602.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62810.16 Da        Isoelectric Point: 4.8340

>NTDB_id=850229 QVG61_RS10540 WP_289930597.1 2285578..2287293(+) (pilB) [Thiohalobacter sp. IOR34]
MVAANPSIQLSGLAKHLVMDGLLAEDAALKACDTAQKEHKHLVSHLVGAQIVSSIDLAKTACKEFGIPLFDLSAMDLEMA
PVKLVDEKLIRQHHALPLFKRGNRLFVAVSDPTNLTALDEIKFHTGITTEPILVEEDKLSKAIEQVLDAADTSMSDLLDE
DLDNLDIVEEDENDDADQESGIDDTPVVRFVNKILLDAINKGASDIHVEPYEKSFRVRLRQDGVLHEVASPPIALAQKIA
ARLKVMSRMDIAERRIPQDGRIKMQLSKNRAIDFRVNTCPTLFGEKIVLRILDPSSAKLGIDALGYEEEQKELYLKNLMK
PYGMILVTGPTGSGKTVSLYTGLNILNTPDRNISTCEDPVEINLSGINQCNMNPKAGFTFAEALRAFLRQDPDVIMVGEI
RDLETAEIAIKAAQTGHMVMSTLHTNDAPQTLARLMNMGVPPYNIASAVNLIIAQRLARRLCNNCKQVDEVPEAALLKEG
FSEEQIAGLKLYKANPDGCDQCTGGYKGRVGIYQVMEISEEMSRIIMEGGNAIQLADQARREGIPDLRESGLKKVIQGIT
SLEEVNRVTKD

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=850229 QVG61_RS10540 WP_289930597.1 2285578..2287293(+) (pilB) [Thiohalobacter sp. IOR34]
ATGGTAGCGGCAAACCCTTCCATCCAACTTAGCGGTCTCGCCAAACACCTTGTCATGGATGGTCTGCTTGCCGAGGATGC
CGCCCTCAAAGCCTGCGATACTGCCCAAAAAGAGCACAAACATCTGGTCAGCCATCTGGTTGGGGCCCAGATAGTATCCA
GCATCGATCTGGCGAAGACAGCCTGCAAGGAATTCGGCATTCCCCTTTTCGACCTGTCCGCGATGGACCTGGAAATGGCA
CCGGTCAAGCTGGTCGACGAAAAGCTGATCCGCCAGCACCATGCCTTGCCACTGTTCAAGCGCGGCAACCGGCTGTTCGT
CGCCGTCTCCGACCCGACCAACCTGACGGCACTGGACGAGATCAAGTTCCACACCGGCATCACCACCGAACCGATCCTGG
TCGAGGAAGACAAACTCAGCAAGGCCATCGAGCAGGTCCTCGATGCCGCCGACACCAGCATGTCGGACCTGCTGGACGAG
GACCTGGACAACCTGGACATCGTCGAGGAGGACGAGAACGACGACGCCGACCAGGAATCCGGTATCGACGACACCCCGGT
GGTCCGCTTCGTCAACAAGATTCTGCTGGACGCCATCAACAAGGGGGCCTCCGACATCCATGTCGAGCCCTACGAGAAGA
GCTTCCGCGTCCGCCTGCGCCAGGACGGCGTGCTGCACGAGGTGGCCTCGCCGCCCATCGCCCTGGCGCAGAAGATCGCC
GCCCGCCTCAAGGTGATGAGCCGCATGGACATCGCCGAGCGCCGCATCCCCCAGGACGGGCGCATCAAGATGCAGCTCTC
CAAGAACCGCGCCATCGACTTCCGCGTCAACACCTGCCCGACCCTGTTCGGGGAGAAGATCGTGCTGCGTATCCTCGACC
CTTCCAGTGCCAAGCTGGGCATCGACGCCCTGGGCTACGAGGAGGAGCAGAAGGAGCTCTATCTCAAGAACCTGATGAAG
CCCTATGGCATGATCCTGGTGACCGGCCCCACGGGTTCGGGCAAGACGGTTTCGCTGTACACCGGGCTGAACATCCTCAA
CACCCCGGACCGCAACATCTCCACCTGCGAGGACCCGGTGGAAATCAACCTCTCCGGCATCAACCAGTGCAACATGAACC
CCAAGGCGGGCTTTACCTTCGCCGAGGCCCTGCGTGCCTTCCTGCGCCAGGACCCGGACGTCATCATGGTCGGTGAGATC
CGCGACCTGGAGACCGCCGAGATCGCCATCAAGGCAGCCCAGACCGGCCACATGGTGATGTCCACCCTGCACACCAACGA
CGCGCCCCAGACCCTGGCGCGGCTGATGAACATGGGCGTGCCGCCGTACAACATCGCCTCGGCGGTCAACCTGATCATCG
CCCAGCGCCTCGCCCGCCGCCTGTGCAACAACTGCAAGCAGGTCGACGAGGTTCCGGAGGCGGCGTTGCTGAAGGAAGGC
TTCAGCGAGGAGCAGATCGCCGGCCTCAAGCTGTACAAGGCCAATCCCGACGGATGTGACCAGTGCACGGGCGGTTACAA
GGGCCGCGTGGGAATCTATCAGGTGATGGAGATCTCCGAGGAGATGTCCCGTATCATCATGGAGGGCGGCAATGCCATCC
AGCTCGCCGACCAGGCGCGCAGGGAGGGCATTCCCGATCTCAGGGAATCCGGGCTGAAGAAGGTCATCCAGGGCATCACC
AGCCTGGAGGAAGTCAACCGAGTCACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(193-460)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.639

100

0.581
  pilB Legionella pneumophila strain ERS1305867

55.382

100

0.559
  pilB Acinetobacter baylyi ADP1

55

100

0.559
  pilF Neisseria gonorrhoeae MS11

50.355

98.774

0.497
  pilB Vibrio cholerae strain A1552

55.709

88.967

0.496
  pilB Vibrio parahaemolyticus RIMD 2210633

53.107

92.995

0.494
  pilB Vibrio campbellii strain DS40M4

51.423

92.294

0.475
  pilB/pilB1 Synechocystis sp. PCC 6803

39.891

96.147

0.384
  pilF Thermus thermophilus HB27

40.962

91.068

0.373
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.583

90.193

0.366