Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   COW1_RS15985 Genome accession   NZ_AP024239
Coordinates   3364574..3365794 (-) Length   406 a.a.
NCBI ID   WP_201344743.1    Uniprot ID   A0A7R7GP94
Organism   Thiohalobacter sp. COW1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3359574..3370794
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  COW1_RS15955 (TspCOW1_31610) - 3359881..3360849 (+) 969 WP_201344737.1 Nudix family hydrolase -
  COW1_RS15960 (TspCOW1_31620) - 3360846..3361337 (+) 492 WP_096367052.1 hypothetical protein -
  COW1_RS15965 (TspCOW1_31630) yacG 3361503..3361703 (-) 201 WP_201344739.1 DNA gyrase inhibitor YacG -
  COW1_RS15970 (TspCOW1_31640) zapD 3361760..3362530 (-) 771 WP_096367050.1 cell division protein ZapD -
  COW1_RS15975 (TspCOW1_31650) coaE 3362850..3363467 (-) 618 WP_096367049.1 dephospho-CoA kinase -
  COW1_RS15980 (TspCOW1_31660) pilD 3363688..3364563 (-) 876 WP_201344741.1 A24 family peptidase Machinery gene
  COW1_RS15985 (TspCOW1_31670) pilC 3364574..3365794 (-) 1221 WP_201344743.1 type II secretion system F family protein Machinery gene
  COW1_RS15990 (TspCOW1_31680) pilB 3365889..3367610 (-) 1722 WP_201344745.1 type IV-A pilus assembly ATPase PilB Machinery gene
  COW1_RS15995 (TspCOW1_31690) - 3367856..3368755 (+) 900 WP_201344747.1 hypothetical protein -
  COW1_RS16000 (TspCOW1_31700) - 3368763..3369719 (-) 957 WP_201344749.1 lysylphosphatidylglycerol synthase transmembrane domain-containing protein -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44353.16 Da        Isoelectric Point: 9.3120

>NTDB_id=84255 COW1_RS15985 WP_201344743.1 3364574..3365794(-) (pilC) [Thiohalobacter sp. COW1]
MAEKALKQDMFVWEGTDRRGNKVKGQTRANNQNLIKADLRRQGITPLKVKKKSALASGRRKKKIEPKDIAIFTRQLATMM
SAGVPLVQAFDIIGRGHENPSMQELVMTIKTDVEGGSNLRDALAKHPLYFDELVCNLVEAGEAAGVLDTLLDKIATYKEK
TEALKSKIKKALFYPTAVIVVAFIVTAILLIFVVPQFEELFAGFGADLPAFTRMVIDLSEFMQSYWWAVFGIIGGLIYGF
MQAKRRSPAFNHTLDKIVLKLPIVGDILTKAAIARYARTLSTMFAAGVPLVEALDSVAGATGNSVYSNAVLRMRDQVSTG
LQLQLAMNQTGLFPNMVVQMVAIGEEAGSLDAMLSKVADFYEQEVDDAVDGLSSLLEPLIMAILGVLVGGLVIAMYLPIF
KMGQVV

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=84255 COW1_RS15985 WP_201344743.1 3364574..3365794(-) (pilC) [Thiohalobacter sp. COW1]
ATGGCAGAGAAAGCGCTCAAACAGGATATGTTCGTCTGGGAAGGCACGGACCGGCGTGGCAACAAGGTCAAGGGCCAGAC
CCGCGCCAATAACCAGAACCTGATCAAGGCCGACCTGCGCCGCCAGGGCATCACCCCGCTCAAGGTGAAGAAGAAATCCG
CCCTGGCCTCGGGCCGCCGGAAGAAGAAGATCGAGCCCAAGGACATCGCCATCTTCACCCGCCAGCTGGCCACCATGATG
TCGGCCGGCGTGCCCCTGGTGCAGGCCTTCGACATCATCGGCCGCGGTCATGAGAACCCCTCCATGCAGGAGCTGGTCAT
GACCATCAAGACCGACGTCGAGGGCGGCAGCAACCTGCGCGACGCCCTGGCCAAACACCCCCTGTATTTCGACGAGCTGG
TGTGCAACCTGGTCGAGGCCGGCGAGGCCGCAGGTGTGCTGGATACGCTGCTGGACAAGATCGCCACCTACAAGGAAAAG
ACCGAGGCGCTGAAGAGCAAGATCAAGAAGGCCCTGTTCTACCCCACGGCGGTTATCGTGGTGGCCTTCATCGTCACCGC
CATCCTGCTGATCTTCGTGGTCCCCCAGTTCGAGGAACTGTTCGCCGGCTTCGGCGCCGATCTGCCGGCCTTCACCCGTA
TGGTGATCGACCTGTCCGAGTTCATGCAGTCCTACTGGTGGGCGGTATTCGGCATCATCGGCGGCCTGATCTATGGTTTC
ATGCAGGCCAAGCGACGCTCGCCCGCCTTCAACCACACACTGGACAAGATCGTACTCAAGCTGCCCATTGTCGGCGACAT
CCTGACCAAGGCCGCCATCGCCCGTTATGCGCGCACCCTGTCCACCATGTTCGCCGCCGGCGTGCCTCTGGTCGAGGCGC
TGGACTCGGTGGCGGGTGCGACCGGCAACAGCGTCTATTCCAATGCCGTGCTGCGCATGCGCGACCAGGTCTCCACCGGT
CTGCAGCTGCAGCTGGCAATGAACCAGACCGGCCTGTTCCCCAATATGGTGGTGCAGATGGTCGCCATCGGCGAGGAGGC
CGGCTCGCTGGACGCCATGTTGAGCAAGGTCGCCGACTTCTACGAGCAGGAGGTCGACGACGCCGTCGATGGCCTGAGCA
GCCTGCTGGAGCCGTTGATCATGGCGATCCTGGGCGTGCTGGTGGGCGGCCTGGTCATCGCCATGTATCTGCCGATCTTC
AAGATGGGCCAGGTCGTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)

Type II secretion system (T2SS), protein F (T2SSF)

(277-398)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7R7GP94

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

61.084

100

0.611
  pilC Legionella pneumophila strain ERS1305867

61.364

97.537

0.599
  pilC Acinetobacter baumannii D1279779

59.012

99.754

0.589
  pilC Acinetobacter baylyi ADP1

58.621

100

0.586
  pilG Neisseria gonorrhoeae MS11

49.38

99.261

0.49
  pilG Neisseria meningitidis 44/76-A

49.38

99.261

0.49
  pilC Vibrio cholerae strain A1552

41.975

99.754

0.419
  pilC Vibrio campbellii strain DS40M4

41.457

98.03

0.406
  pilC Thermus thermophilus HB27

38.795

100

0.397

Multiple sequence alignment