Detailed information NTG0084254

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: COW1_RS15980
• Genome accession: NZ_AP024239
• Coordinates: 3363688..3364563 (-)
• Length: 291 a.a.
• NCBI ID: WP_201344741.1
• Uniprot ID: A0A7R7GPY4
• Organism: Thiohalobacter sp. COW1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3358688..3369563

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
COW1_RS15950 (TspCOW1_31600)	argJ	3358698..3359912 (+)	1215	WP_201344735.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-
COW1_RS15955 (TspCOW1_31610)	-	3359881..3360849 (+)	969	WP_201344737.1	Nudix family hydrolase	-
COW1_RS15960 (TspCOW1_31620)	-	3360846..3361337 (+)	492	WP_096367052.1	hypothetical protein	-
COW1_RS15965 (TspCOW1_31630)	yacG	3361503..3361703 (-)	201	WP_201344739.1	DNA gyrase inhibitor YacG	-
COW1_RS15970 (TspCOW1_31640)	zapD	3361760..3362530 (-)	771	WP_096367050.1	cell division protein ZapD	-
COW1_RS15975 (TspCOW1_31650)	coaE	3362850..3363467 (-)	618	WP_096367049.1	dephospho-CoA kinase	-
COW1_RS15980 (TspCOW1_31660)	pilD	3363688..3364563 (-)	876	WP_201344741.1	A24 family peptidase	Machinery gene
COW1_RS15985 (TspCOW1_31670)	pilC	3364574..3365794 (-)	1221	WP_201344743.1	type II secretion system F family protein	Machinery gene
COW1_RS15990 (TspCOW1_31680)	pilB	3365889..3367610 (-)	1722	WP_201344745.1	type IV-A pilus assembly ATPase PilB	Machinery gene
COW1_RS15995 (TspCOW1_31690)	-	3367856..3368755 (+)	900	WP_201344747.1	hypothetical protein	-

Sequence

Protein

• Length: 291 a.a.
• Molecular weight: 31684.74 Da
• Isoelectric Point: 6.4673

>NTDB_id=84254 COW1_RS15980 WP_201344741.1 3363688..3364563(-) (pilD) [Thiohalobacter sp. COW1]
MELLELIRTSPGWLIGVCALLGLVVGSFLNVVAHRLPLMMQREWESQCRELLESPAAGDTTTGSFNLVTPASRCPYCGHR
IRAIENIPVISWLFLRGRCGECRTPISVKYPLVEAFTGLTAGLLAWHFGFGWPLAAALVFTWVLIALSVIDIEHQLLPDS
LTLALLWLGLLLSLGEVFVGPEQAIIGAAAGYLALWTVYQLFRLLTGKEGMGFGDFKLLAACGAWMGWKLLPLIILLSSL
VGAVVGVAMILILGRDRQLPIPFGPYIAAAGWIALLWGDDLIQGYLRFAGL

Nucleotide

• Length: 876 bp

>NTDB_id=84254 COW1_RS15980 WP_201344741.1 3363688..3364563(-) (pilD) [Thiohalobacter sp. COW1]
ATGGAACTGCTGGAACTCATCCGGACCAGTCCGGGCTGGCTGATCGGCGTCTGTGCGCTGCTGGGCCTGGTGGTCGGCAG
CTTTCTCAACGTGGTCGCCCACCGCCTGCCGCTGATGATGCAGCGCGAGTGGGAGTCGCAGTGCCGGGAACTGCTCGAAT
CCCCGGCCGCGGGCGATACGACCACGGGCAGCTTCAATCTCGTCACTCCCGCATCACGCTGCCCCTACTGCGGCCACCGC
ATCCGTGCCATCGAGAACATCCCGGTCATCAGCTGGCTGTTCCTGCGCGGCCGCTGCGGCGAGTGCCGGACACCCATCTC
CGTCAAATACCCGCTGGTCGAGGCCTTCACCGGCCTGACCGCGGGGCTGCTGGCCTGGCATTTCGGCTTCGGCTGGCCGC
TGGCCGCCGCCCTGGTCTTCACCTGGGTGCTGATCGCCCTGAGCGTGATCGACATCGAGCACCAGCTGCTGCCCGACTCG
CTCACCCTGGCCCTGCTCTGGCTGGGTCTGCTGCTGAGTCTGGGCGAGGTCTTCGTCGGTCCGGAACAGGCCATCATCGG
CGCTGCTGCCGGCTATCTCGCACTGTGGACGGTGTACCAGCTGTTCCGGCTGCTCACCGGCAAGGAGGGCATGGGCTTCG
GCGACTTCAAACTGCTCGCCGCCTGCGGCGCCTGGATGGGCTGGAAGCTGCTGCCGCTGATCATCCTGCTCTCCTCGCTC
GTCGGCGCCGTGGTCGGTGTGGCGATGATCCTGATCCTCGGCCGCGACCGCCAGCTGCCCATCCCGTTCGGCCCCTACAT
CGCTGCCGCCGGCTGGATCGCGCTGCTGTGGGGCGACGATCTCATCCAGGGTTATCTGCGCTTCGCCGGGTTATAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (138-247)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-128)

3D structure

• AlphaFold DB: A0A7R7GPY4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	52.448	98.282	0.515
pilD	Acinetobacter baumannii D1279779	51.493	92.096	0.474
pilD	Acinetobacter nosocomialis M2	51.119	92.096	0.471
pilD	Vibrio campbellii strain DS40M4	47.887	97.595	0.467
pilD	Neisseria gonorrhoeae MS11	46.816	91.753	0.43