Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   COW1_RS15990 Genome accession   NZ_AP024239
Coordinates   3365889..3367610 (-) Length   573 a.a.
NCBI ID   WP_201344745.1    Uniprot ID   A0A7R7JDP1
Organism   Thiohalobacter sp. COW1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3360889..3372610
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  COW1_RS15965 (TspCOW1_31630) yacG 3361503..3361703 (-) 201 WP_201344739.1 DNA gyrase inhibitor YacG -
  COW1_RS15970 (TspCOW1_31640) zapD 3361760..3362530 (-) 771 WP_096367050.1 cell division protein ZapD -
  COW1_RS15975 (TspCOW1_31650) coaE 3362850..3363467 (-) 618 WP_096367049.1 dephospho-CoA kinase -
  COW1_RS15980 (TspCOW1_31660) pilD 3363688..3364563 (-) 876 WP_201344741.1 A24 family peptidase Machinery gene
  COW1_RS15985 (TspCOW1_31670) pilC 3364574..3365794 (-) 1221 WP_201344743.1 type II secretion system F family protein Machinery gene
  COW1_RS15990 (TspCOW1_31680) pilB 3365889..3367610 (-) 1722 WP_201344745.1 type IV-A pilus assembly ATPase PilB Machinery gene
  COW1_RS15995 (TspCOW1_31690) - 3367856..3368755 (+) 900 WP_201344747.1 hypothetical protein -
  COW1_RS16000 (TspCOW1_31700) - 3368763..3369719 (-) 957 WP_201344749.1 lysylphosphatidylglycerol synthase transmembrane domain-containing protein -
  COW1_RS16005 - 3369754..3370893 (-) 1140 WP_201344751.1 glycosyltransferase family 4 protein -
  COW1_RS16010 (TspCOW1_31740) - 3370915..3371760 (-) 846 WP_201344753.1 glycosyltransferase family 2 protein -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 63389.73 Da        Isoelectric Point: 4.7080

>NTDB_id=84256 COW1_RS15990 WP_201344745.1 3365889..3367610(-) (pilB) [Thiohalobacter sp. COW1]
MVSPNPKIQLSGLARRLVLDEIISEPDMLQALETANRDKQPLVKYLVGNKICTSRQVANAACTEFGVPLFDLSVMDMETA
PLKLVDEKLIRQHNALPLFKRGNRLFVAVSDPTNLAALDEIKFHTGITTEPILVEEDKLTKVLDSAMDAMDSTMSDLMDD
DLDNLDIQAGDEDDAGQDLDSSEVDDTPVVRFVNKILLDAINKGASDIHIEPYEKTFRVRIRQDGVLHEVANPPVNLANK
IAARVKVMSRMDIAERRVPQDGRIKMQLSKNRAIDFRVNTCPTLFGEKIVLRILDPSSAKLGIDALGYEEHQKQLYMENL
NKPYGMILVTGPTGSGKTVSLYTGLNILNTPDRNISTCEDPVEINLPGINQCNMNPKTGFTFAEALRAFLRQDPDIIMVG
EIRDLETAEIAIKAAQTGHMVMSTLHTNDAPQTLARLMNMGVPPYNIASAVNLIIAQRLARRLCNNCKQVDDVPEAALLE
EGFKQEDVSGLKLYKANPEGCDQCTGGYKGRVGIYQVMEISEEMSRIIMDGGNALQLADQAKKEGIMDLRECGLEKVRQG
ITGLEEVNRVTKD

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=84256 COW1_RS15990 WP_201344745.1 3365889..3367610(-) (pilB) [Thiohalobacter sp. COW1]
ATGGTATCACCGAATCCAAAAATCCAGCTCAGCGGCCTGGCGCGCCGCCTGGTGCTGGACGAAATCATCTCTGAACCCGA
CATGCTCCAGGCCCTGGAGACGGCCAACCGCGACAAGCAGCCGCTGGTCAAATACCTGGTCGGCAACAAGATCTGCACCA
GCCGGCAGGTGGCCAATGCCGCCTGTACCGAGTTCGGCGTGCCGCTGTTCGACCTGTCGGTCATGGACATGGAGACGGCC
CCGCTCAAGCTGGTGGATGAGAAGCTCATCCGACAGCACAACGCCCTGCCCCTGTTCAAGCGCGGCAACCGGCTGTTCGT
CGCCGTCTCCGACCCCACCAACCTGGCCGCCCTGGACGAGATCAAGTTCCACACCGGCATCACCACCGAACCCATTCTGG
TCGAGGAGGACAAGCTCACCAAGGTCCTGGACTCGGCCATGGACGCCATGGACAGCACTATGTCCGATCTCATGGACGAC
GATCTGGACAATCTGGATATCCAGGCAGGTGACGAGGATGACGCCGGCCAGGACCTCGATTCCTCCGAGGTGGACGACAC
CCCGGTGGTGCGTTTCGTCAACAAGATCCTGCTGGACGCCATCAACAAAGGGGCGTCGGACATCCACATCGAGCCCTACG
AGAAGACCTTCCGGGTGCGTATCCGCCAGGACGGCGTGCTGCACGAGGTGGCCAACCCCCCGGTGAACCTGGCCAACAAG
ATCGCCGCCCGCGTCAAGGTCATGAGCCGCATGGACATCGCCGAGCGCCGCGTCCCCCAGGACGGGCGCATCAAGATGCA
GCTGTCCAAGAACCGCGCCATCGACTTTCGTGTGAATACCTGTCCGACCCTGTTCGGCGAGAAGATCGTACTGCGTATCC
TCGACCCCAGCAGCGCCAAGCTGGGCATCGACGCCCTGGGCTACGAGGAGCACCAGAAACAACTCTACATGGAGAACCTG
AACAAGCCCTACGGCATGATTCTGGTCACCGGCCCGACCGGTTCGGGTAAGACGGTCTCGCTCTACACCGGCCTGAATAT
CCTCAACACACCGGATCGCAACATCTCCACCTGCGAGGACCCGGTGGAAATCAACCTGCCGGGCATCAACCAGTGCAACA
TGAACCCCAAGACCGGGTTCACCTTCGCCGAGGCCCTGCGCGCCTTCCTGCGCCAGGACCCGGACATCATCATGGTGGGT
GAGATCCGTGACCTGGAAACCGCCGAGATCGCCATCAAGGCGGCCCAGACCGGTCACATGGTGATGTCCACCCTGCACAC
CAACGATGCGCCCCAGACCCTGGCCCGCCTGATGAACATGGGGGTGCCGCCCTACAACATCGCCTCGGCCGTGAACCTGA
TCATCGCGCAGCGCCTGGCCCGGCGCCTGTGCAACAACTGCAAGCAGGTCGATGATGTGCCCGAGGCCGCCCTGCTGGAA
GAGGGCTTCAAGCAGGAGGACGTCTCGGGGCTGAAGCTCTACAAGGCCAATCCCGAAGGTTGTGACCAGTGCACGGGCGG
CTACAAGGGCCGCGTTGGTATATATCAGGTCATGGAGATCTCCGAGGAGATGTCCCGCATCATCATGGACGGCGGCAACG
CCCTCCAGCTCGCCGACCAGGCGAAGAAGGAAGGCATCATGGACCTGCGCGAATGCGGGCTGGAGAAGGTCAGGCAGGGC
ATCACCGGCCTGGAAGAGGTCAACCGCGTGACCAAGGACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(195-462)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7R7JDP1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.979

99.127

0.585
  pilB Legionella pneumophila strain ERS1305867

56.304

100

0.569
  pilB Acinetobacter baylyi ADP1

57.699

98.604

0.569
  pilF Neisseria gonorrhoeae MS11

49.647

98.778

0.49
  pilB Vibrio cholerae strain A1552

54.492

89.354

0.487
  pilB Vibrio parahaemolyticus RIMD 2210633

51.796

92.321

0.478
  pilB Vibrio campbellii strain DS40M4

50.473

92.321

0.466
  pilF Thermus thermophilus HB27

39.543

99.302

0.393
  pilB/pilB1 Synechocystis sp. PCC 6803

40

96.859

0.387
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.394

97.906

0.386

Multiple sequence alignment