Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QBS06_RS01385 Genome accession   NZ_CP121798
Coordinates   140330..141439 (+) Length   369 a.a.
NCBI ID   WP_041216515.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain YURI 17     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 135330..146439
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBS06_RS01350 - 135946..136257 (+) 312 WP_041216519.1 hypothetical protein -
  QBS06_RS01355 - 136314..136733 (-) 420 WP_041216518.1 DUF4426 domain-containing protein -
  QBS06_RS01360 yggU 136752..137051 (-) 300 WP_041216517.1 DUF167 family protein YggU -
  QBS06_RS01365 - 137051..137602 (-) 552 WP_016351969.1 YggT family protein -
  QBS06_RS01370 proC 137643..138467 (-) 825 WP_041216516.1 pyrroline-5-carboxylate reductase -
  QBS06_RS01375 - 138526..139227 (-) 702 WP_029304817.1 YggS family pyridoxal phosphate-dependent enzyme -
  QBS06_RS01380 pilT 139267..140301 (+) 1035 WP_011707389.1 type IVa pilus ATPase TapT Machinery gene
  QBS06_RS01385 pilU 140330..141439 (+) 1110 WP_041216515.1 type IVa pilus ATPase TapU Machinery gene
  QBS06_RS01390 yaaA 141471..142244 (+) 774 WP_041216514.1 peroxide stress protein YaaA -
  QBS06_RS01395 srmB 142421..143644 (-) 1224 WP_279451605.1 ATP-dependent RNA helicase SrmB -
  QBS06_RS01400 - 143773..144483 (+) 711 WP_041216513.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  QBS06_RS01405 brnQ 144675..145982 (+) 1308 WP_041216559.1 branched-chain amino acid transport system II carrier protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41037.18 Da        Isoelectric Point: 6.5035

>NTDB_id=814272 QBS06_RS01385 WP_041216515.1 140330..141439(+) (pilU) [Aeromonas hydrophila strain YURI 17]
MNLDALLTELVARKGSDLFITVGTPPTLKVNGHLQSLAEAPLDKAKALALVKETLSQEHFERYLHTKEANYAIHREELGR
FRVSAFWQQELPGMVLRRIETRIPTFDELALPPILQEIALAKRGLVLFVGATGAGKSTTQAAMIGYRNQHVDGHILTVED
PVEFVHQHGRSLITQREVGIDTASFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSLDGRRRCAAFEVLLNTPLITDIVRKGEMHRLKEVMTKSTELGMQTFDQA
LFALFCTGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=814272 QBS06_RS01385 WP_041216515.1 140330..141439(+) (pilU) [Aeromonas hydrophila strain YURI 17]
ATGAATCTGGATGCCCTGCTGACCGAGCTGGTCGCCCGCAAAGGCTCGGATCTGTTCATCACAGTCGGCACGCCGCCGAC
CCTCAAGGTCAACGGCCACCTGCAGTCACTGGCAGAAGCCCCCCTCGACAAGGCCAAGGCACTGGCGCTGGTCAAGGAGA
CCCTGAGCCAGGAGCACTTCGAGCGCTACCTGCACACCAAGGAGGCCAACTACGCCATCCACCGCGAGGAGCTTGGCCGC
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCGGGCATGGTGCTGCGCCGTATCGAGACCCGCATCCCCACCTTCGA
CGAGCTGGCGCTGCCCCCCATCCTGCAGGAGATCGCCCTGGCCAAGCGCGGGCTGGTGCTGTTCGTCGGTGCCACCGGCG
CCGGCAAATCAACCACCCAGGCGGCCATGATCGGCTATCGCAACCAGCATGTGGACGGCCACATCCTGACGGTGGAAGAT
CCGGTAGAGTTCGTGCATCAGCACGGCCGCAGCCTGATCACCCAGCGCGAGGTGGGCATAGACACAGCGTCGTTCGATGT
GGCGCTGAAGAGTTCGCTGCGCCAGGCCCCTGACGTGATCCTGATCGGTGAAATCCGCAGCCAGGAGACCATGGAGTTTG
CCCTGCAGTTTGCCGAGACCGGCCACCTCTGCCTCGCCACCCTGCACGCCAACAATGCCAACCAGGCGCTGGACCGCATC
CTGCACCTGGTCCCTCAGGAGAAGCACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGTGCCATCGTGGCCCAGCA
ACTGCTGCCCAGCCTGGACGGACGCCGCCGCTGTGCGGCCTTCGAGGTGCTGCTCAACACCCCGCTCATCACCGATATCG
TCCGCAAGGGGGAGATGCACCGGCTCAAGGAGGTGATGACCAAGTCCACCGAGCTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCGCCCTGTTCTGTACCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTGTCGGGGCGCGAGCAGCTGGGTGCAGGCACTCTCGACAACGTCACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.326

99.729

0.602
  pilU Pseudomonas stutzeri DSM 10701

59.259

95.122

0.564
  pilU Acinetobacter baylyi ADP1

56.941

95.664

0.545
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.994

89.702

0.377
  pilT Legionella pneumophila strain Lp02

40.233

92.954

0.374
  pilT Legionella pneumophila strain ERS1305867

40.233

92.954

0.374
  pilT Acinetobacter baylyi ADP1

39.003

92.412

0.36
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.515

0.36
  pilT Vibrio cholerae strain A1552

39.82

90.515

0.36