Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QBS06_RS01380 Genome accession   NZ_CP121798
Coordinates   139267..140301 (+) Length   344 a.a.
NCBI ID   WP_011707389.1    Uniprot ID   A0A081UZ58
Organism   Aeromonas hydrophila strain YURI 17     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 134267..145301
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBS06_RS01350 - 135946..136257 (+) 312 WP_041216519.1 hypothetical protein -
  QBS06_RS01355 - 136314..136733 (-) 420 WP_041216518.1 DUF4426 domain-containing protein -
  QBS06_RS01360 yggU 136752..137051 (-) 300 WP_041216517.1 DUF167 family protein YggU -
  QBS06_RS01365 - 137051..137602 (-) 552 WP_016351969.1 YggT family protein -
  QBS06_RS01370 proC 137643..138467 (-) 825 WP_041216516.1 pyrroline-5-carboxylate reductase -
  QBS06_RS01375 - 138526..139227 (-) 702 WP_029304817.1 YggS family pyridoxal phosphate-dependent enzyme -
  QBS06_RS01380 pilT 139267..140301 (+) 1035 WP_011707389.1 type IVa pilus ATPase TapT Machinery gene
  QBS06_RS01385 pilU 140330..141439 (+) 1110 WP_041216515.1 type IVa pilus ATPase TapU Machinery gene
  QBS06_RS01390 yaaA 141471..142244 (+) 774 WP_041216514.1 peroxide stress protein YaaA -
  QBS06_RS01395 srmB 142421..143644 (-) 1224 WP_279451605.1 ATP-dependent RNA helicase SrmB -
  QBS06_RS01400 - 143773..144483 (+) 711 WP_041216513.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38287.06 Da        Isoelectric Point: 6.7439

>NTDB_id=814271 QBS06_RS01380 WP_011707389.1 139267..140301(+) (pilT) [Aeromonas hydrophila strain YURI 17]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALEHREVHALIYDIMNDHQRKELEENFEVDFSFEVPNLAR
FRVNAFQQARGSGAVFRTIPSTVLSLEDLDAPEIFRKIAEYPRGLVLVTGPTGSGKSTTLAAMVNYINENFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGAEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMMGIPAVRNLIREDKIAQLYSVIQTGMTHGMQTMDQSL
KQLVSRGVVAALDAKAKAVDPNTI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=814271 QBS06_RS01380 WP_011707389.1 139267..140301(+) (pilT) [Aeromonas hydrophila strain YURI 17]
ATGGATATCACAGAGTTATTGGCTTTCAGTGTAAAACATAAAGCCTCGGATCTACACCTCTCGGCCGGGGTTCCCCCGAT
GATCAGGGTTGATGGCGAGGTTCGCAAGATCAATTTGCCCGCCCTGGAACACCGGGAAGTGCACGCCCTCATCTACGACA
TCATGAACGACCACCAGCGCAAGGAACTGGAAGAGAACTTCGAGGTGGACTTCTCGTTCGAGGTGCCAAACCTCGCCCGC
TTCCGGGTCAACGCCTTCCAGCAGGCGCGCGGCTCCGGCGCGGTATTTCGGACCATCCCCAGCACTGTGCTGAGCCTCGA
AGATCTGGACGCGCCGGAGATCTTCCGCAAGATTGCCGAATACCCGCGCGGCCTGGTGCTGGTGACCGGTCCGACCGGCT
CGGGTAAATCCACCACCCTGGCGGCCATGGTCAACTACATCAACGAGAACTTTCATCACCACATCCTCACCATCGAGGAT
CCCATCGAATTCGTGCACGAGAACAAGCGCTGCCTGGTGAACCAGCGGGAAGTACACCGCGACACCAAGAGCTTCAGCAA
CGCCCTGCGCTCGGCGCTGCGGGAAGACCCGGACATCATCCTGGTGGGCGAGATGCGCGATCTGGAAACCATTCGTCTGG
CCATGACCGCGGCCGAGACCGGCCACCTGGTGTTCGGCACCCTGCACACCTCGTCGGCGGCCAAGACCATCGACCGGATC
ATCGACGTCTTCCCCGGTGCGGAAAAAGACATGGTGCGCTCCATGCTCTCCGAGTCGCTGCGGGCGGTCATCTCCCAGAC
CCTGCTCAAGCGCATCGGCGGCGGCCGGGTGGCGGCCCACGAGATCATGATGGGTATTCCCGCCGTGCGTAACCTCATCC
GGGAAGACAAGATCGCCCAGCTCTACTCGGTGATCCAGACCGGGATGACCCACGGCATGCAGACCATGGATCAGAGCCTC
AAGCAGCTGGTGAGTCGCGGCGTAGTGGCGGCGCTGGATGCCAAGGCCAAGGCGGTCGATCCCAACACCATCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A081UZ58

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.326

100

0.773
  pilT Acinetobacter baylyi ADP1

77.326

100

0.773
  pilT Acinetobacter baumannii D1279779

77.035

100

0.77
  pilT Acinetobacter baumannii strain A118

77.035

100

0.77
  pilT Pseudomonas stutzeri DSM 10701

76.833

99.128

0.762
  pilT Pseudomonas aeruginosa PAK

75.953

99.128

0.753
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

76.558

97.965

0.75
  pilT Vibrio cholerae strain A1552

76.558

97.965

0.75
  pilT Legionella pneumophila strain Lp02

72.647

98.837

0.718
  pilT Legionella pneumophila strain ERS1305867

72.647

98.837

0.718
  pilT Neisseria meningitidis 8013

70.796

98.547

0.698
  pilT Neisseria gonorrhoeae MS11

70.501

98.547

0.695
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.754

99.419

0.515
  pilU Vibrio cholerae strain A1552

42.899

100

0.43
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407