Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QBE03_RS08175 Genome accession   NZ_CP121698
Coordinates   1896391..1897521 (+) Length   376 a.a.
NCBI ID   WP_279363381.1    Uniprot ID   -
Organism   Xanthomonas sacchari strain DJ16     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1891391..1902521
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBE03_RS08150 - 1891548..1892000 (-) 453 WP_279363376.1 DUF4426 domain-containing protein -
  QBE03_RS08155 proC 1892143..1892988 (-) 846 WP_279363378.1 pyrroline-5-carboxylate reductase -
  QBE03_RS08160 - 1893117..1894145 (-) 1029 WP_017913913.1 hypothetical protein -
  QBE03_RS08165 - 1894462..1895139 (-) 678 WP_279363959.1 YggS family pyridoxal phosphate-dependent enzyme -
  QBE03_RS08170 pilT 1895240..1896277 (+) 1038 WP_010344138.1 type IV pilus twitching motility protein PilT Machinery gene
  QBE03_RS08175 pilU 1896391..1897521 (+) 1131 WP_279363381.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QBE03_RS08180 - 1897678..1898940 (+) 1263 WP_017913914.1 WD40 repeat domain-containing protein -
  QBE03_RS08185 - 1898937..1900436 (+) 1500 WP_279363384.1 diguanylate cyclase -
  QBE03_RS08190 - 1900865..1901461 (+) 597 WP_152236607.1 MFS transporter permease -
  QBE03_RS08195 - 1901614..1902330 (+) 717 WP_279363387.1 YitT family protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41906.19 Da        Isoelectric Point: 6.8811

>NTDB_id=814004 QBE03_RS08175 WP_279363381.1 1896391..1897521(+) (pilU) [Xanthomonas sacchari strain DJ16]
MSTLDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKITPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECNFAIGVAG
VGRFRVSCFYQRNQVGMVLRRIETRIPSVDELNLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAISFAETGHLVLCTLHANNANQAM
DRVINFFPEDRRSQLLMDLSLNLRGVVAQQLIPTPDGKGRRVAMEIMLGTPLVQDYIREGEIHKLKDVMKESTNLGMKTF
DQSLFELYQAGEISYEDALRHADSQNEVRLRIKLAQGGDARTLAQGLDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=814004 QBE03_RS08175 WP_279363381.1 1896391..1897521(+) (pilU) [Xanthomonas sacchari strain DJ16]
ATGAGCACCCTCGACTTCACCTCCTTCCTGAAACTGATGGCGCACCAGAAGGCGTCGGACCTGTTCATCACCTCGGGCAT
GCCGCCGGCGATCAAGGTGCACGGCAAGATCACCCCGATCACGCAGACGCCGCTGACCCCGCAGCAGAGCCGCGACCTGG
TGCTGAACGTGATGACGCCGGCGCAGCGCGAGGAGTTCGAGAAGACCCACGAGTGCAACTTCGCCATCGGCGTGGCCGGG
GTCGGGCGCTTCCGCGTCAGCTGCTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCGCCGGATCGAGACGCGCATTCC
CAGCGTGGACGAGCTGAACCTGCCGCCGGTGATCAAGACCCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGCACCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAACTCCACCGGGCACATCATCACC
ATCGAGGACCCGATCGAGTTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAGGTCGGCATCGATACCGACAG
CTGGGAGAACGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAGGGCA
TGGACCATGCCATCTCCTTCGCCGAGACCGGCCACCTGGTGCTGTGCACCCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCGTCATCAACTTCTTCCCCGAGGACCGTCGCAGCCAACTGCTGATGGACCTGTCGCTGAACCTGCGCGGCGTGGT
CGCGCAGCAGCTGATCCCGACCCCGGACGGCAAGGGCCGCCGCGTGGCCATGGAGATCATGCTCGGTACGCCGCTGGTGC
AGGACTACATCCGCGAGGGCGAGATCCACAAGCTCAAGGACGTGATGAAGGAGTCCACCAACCTGGGCATGAAGACCTTC
GACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGAGGATGCGCTGCGCCACGCCGATTCGCAGAACGA
GGTGCGCCTGCGCATCAAGCTGGCGCAGGGCGGCGACGCGCGCACCCTGGCGCAGGGCCTGGACGGGGTCGAGATCGCCG
AGGTGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.213

97.606

0.646
  pilU Acinetobacter baylyi ADP1

63.585

94.947

0.604
  pilU Vibrio cholerae strain A1552

53.09

94.681

0.503
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.714

93.085

0.388
  pilT Pseudomonas aeruginosa PAK

41.003

90.16

0.37
  pilT Acinetobacter baumannii strain A118

40.407

91.489

0.37
  pilT Acinetobacter nosocomialis M2

40.407

91.489

0.37
  pilT Acinetobacter baumannii D1279779

40.407

91.489

0.37
  pilT Legionella pneumophila strain ERS1305867

40.597

89.096

0.362
  pilT Legionella pneumophila strain Lp02

40.597

89.096

0.362