Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   P3G65_RS01000 Genome accession   NZ_CP120417
Coordinates   185766..186710 (+) Length   314 a.a.
NCBI ID   WP_019291447.1    Uniprot ID   -
Organism   Lactococcus sp. bn62     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 180766..191710
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P3G65_RS00985 (P3G65_00990) - 182027..183628 (-) 1602 WP_019291792.1 GH25 family lysozyme -
  P3G65_RS00990 (P3G65_00995) guaB 183797..185278 (+) 1482 WP_003133714.1 IMP dehydrogenase -
  P3G65_RS00995 (P3G65_01000) rpsU 185404..185580 (+) 177 WP_003134539.1 30S ribosomal protein S21 -
  P3G65_RS01000 (P3G65_01005) comGA 185766..186710 (+) 945 WP_019291447.1 competence type IV pilus ATPase ComGA Machinery gene
  P3G65_RS01005 (P3G65_01010) comGB 186649..187668 (+) 1020 Protein_187 competence type IV pilus assembly protein ComGB -
  P3G65_RS01010 (P3G65_01015) comYC 187687..187992 (+) 306 WP_029345656.1 competence type IV pilus major pilin ComGC Machinery gene
  P3G65_RS01015 (P3G65_01020) comGD 187967..188383 (+) 417 WP_019291446.1 competence type IV pilus minor pilin ComGD Machinery gene
  P3G65_RS01020 (P3G65_01025) comGE 188349..188648 (+) 300 WP_019291445.1 competence type IV pilus minor pilin ComGE -
  P3G65_RS01025 (P3G65_01030) comGF 188629..189069 (+) 441 WP_019291444.1 competence type IV pilus minor pilin ComGF -
  P3G65_RS01030 (P3G65_01035) - 189301..190146 (+) 846 WP_284071234.1 metal ABC transporter solute-binding protein, Zn/Mn family -
  P3G65_RS01035 (P3G65_01040) - 190217..191101 (-) 885 WP_019291442.1 RluA family pseudouridine synthase -

Sequence

Protein


Download         Length: 314 a.a.        Molecular weight: 35713.01 Da        Isoelectric Point: 6.9700

>NTDB_id=805536 P3G65_RS01000 WP_019291447.1 185766..186710(+) (comGA) [Lactococcus sp. bn62]
MIQVLAKNLLQKAVDQMVHDLYLVALEGKYSLYFRTTTERRFEGELTLEQGQALIAHMKFLSGMNLGESRRVQLGACTYI
LDKGEQRLRLSTVGDFHGQESLVIRLLHHQQNQLHFWNSEIFNTIIGGRGLYLFSGPVGSGKTSLMYKFAREHFKNQQVI
CIEDPVELVENEFLQLQVNKVIGNDYDSLIKLSLRHRPDLLIVGEIRDSQTAKAVLRASLTGYTVFSTVHARSISGVIAR
LKELGLSDWELQSSLQRVIYQRLIAGKGLLVCEKEKFEEWKPDEWNDQIDQLVADGFISAVAAAREKIEFSKAD

Nucleotide


Download         Length: 945 bp        

>NTDB_id=805536 P3G65_RS01000 WP_019291447.1 185766..186710(+) (comGA) [Lactococcus sp. bn62]
ATGATACAGGTTTTGGCAAAAAACCTTTTACAAAAAGCAGTAGATCAGATGGTGCACGATCTTTATTTAGTAGCATTAGA
AGGAAAGTATTCTCTCTATTTTCGTACAACAACAGAAAGGAGGTTTGAAGGAGAACTCACCCTTGAGCAAGGGCAAGCAC
TGATTGCTCATATGAAATTTTTATCTGGTATGAATCTAGGAGAAAGTAGACGAGTACAGTTGGGGGCTTGCACTTATATC
TTAGATAAAGGTGAGCAGCGCTTACGTTTATCTACTGTCGGTGATTTTCATGGTCAAGAAAGCTTGGTTATTCGACTCTT
GCATCATCAACAAAACCAACTTCATTTTTGGAATTCAGAGATCTTTAATACTATTATTGGGGGAAGAGGCCTTTACCTTT
TTTCAGGTCCTGTAGGTTCAGGTAAGACCAGTCTTATGTACAAATTTGCAAGGGAGCATTTTAAAAATCAACAAGTTATT
TGTATAGAAGATCCTGTGGAGTTAGTAGAAAATGAATTTTTGCAACTTCAAGTCAATAAAGTAATAGGGAATGATTATGA
TTCCTTGATTAAGCTTTCGCTGCGACACAGACCTGATTTACTTATTGTTGGTGAAATTAGGGATAGTCAAACGGCAAAAG
CTGTTCTTCGTGCAAGTTTGACTGGGTATACAGTATTCTCTACTGTTCATGCACGCTCAATATCAGGTGTAATTGCACGC
TTAAAAGAACTTGGATTAAGTGATTGGGAGCTACAATCCAGTCTTCAGCGGGTGATTTATCAACGATTAATTGCAGGAAA
GGGATTGCTTGTTTGTGAAAAGGAAAAGTTTGAAGAATGGAAACCAGACGAATGGAATGACCAGATTGATCAGTTGGTTG
CAGATGGATTTATCTCAGCTGTTGCAGCTGCGCGCGAAAAAATTGAGTTTAGCAAAGCAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

60.191

100

0.602
  comYA Streptococcus gordonii str. Challis substr. CH1

53.651

100

0.538
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

53.035

99.682

0.529
  comGA/cglA/cilD Streptococcus pneumoniae R6

52.716

99.682

0.525
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

52.716

99.682

0.525
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

52.716

99.682

0.525
  comGA/cglA/cilD Streptococcus pneumoniae D39

52.716

99.682

0.525
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

52.564

99.363

0.522
  comYA Streptococcus mutans UA159

50.949

100

0.513
  comYA Streptococcus mutans UA140

50.949

100

0.513