Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   P2C01_RS02005 Genome accession   NZ_CP119521
Coordinates   413165..414271 (+) Length   368 a.a.
NCBI ID   WP_275723618.1    Uniprot ID   -
Organism   Vibrio furnissii strain C1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 408165..419271
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P2C01_RS01970 - 408516..409115 (-) 600 WP_055467258.1 XTP/dITP diphosphatase -
  P2C01_RS01975 - 409139..409570 (-) 432 WP_014205827.1 DUF4426 domain-containing protein -
  P2C01_RS01980 yggU 409628..409921 (-) 294 WP_038150700.1 DUF167 family protein YggU -
  P2C01_RS01985 - 409918..410475 (-) 558 WP_004724262.1 YggT family protein -
  P2C01_RS01990 proC 410527..411345 (-) 819 WP_004724261.1 pyrroline-5-carboxylate reductase -
  P2C01_RS01995 - 411383..412090 (-) 708 WP_275723615.1 YggS family pyridoxal phosphate-dependent enzyme -
  P2C01_RS02000 pilT 412116..413153 (+) 1038 WP_004724259.1 type IV pilus twitching motility protein PilT Machinery gene
  P2C01_RS02005 pilU 413165..414271 (+) 1107 WP_275723618.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  P2C01_RS02010 ruvX 414368..414793 (-) 426 WP_004724257.1 Holliday junction resolvase RuvX -
  P2C01_RS02015 - 414856..415419 (-) 564 WP_004724256.1 YqgE/AlgH family protein -
  P2C01_RS02020 gshB 415458..416414 (-) 957 WP_004724255.1 glutathione synthase -
  P2C01_RS02025 rsmE 416427..417158 (-) 732 WP_004724254.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  P2C01_RS02030 - 417266..417967 (-) 702 WP_004724253.1 endonuclease -
  P2C01_RS02035 - 418106..418606 (-) 501 WP_275723622.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41387.44 Da        Isoelectric Point: 6.4509

>NTDB_id=799440 P2C01_RS02005 WP_275723618.1 413165..414271(+) (pilU) [Vibrio furnissii strain C1]
MQLEECLQGMMTHKASDLYITVGAPVLYRVDGELRAHGDALNEAQVRTLLAAMMDDTRHADYLATREANFAIVRDLGRFR
VSAFFQRELPGAVIRRIETQIPTFDELKLPPVLKDLSIAKRGLVLVVGATGSGKSTTMAAMTGYRNMHRTGHILTVEDPI
EFVHEHQRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQREQFLFDLSMNLKGVVGQQLIRDKNGQGRHGVFEVLLNSPRVSDIIRRGELHELKNTMAKSKEVGMQTFDQALY
QLVVEHKISEQDALHSADSANDLRLMLKMQRGDVTSSGSLDHVQIDME

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=799440 P2C01_RS02005 WP_275723618.1 413165..414271(+) (pilU) [Vibrio furnissii strain C1]
ATGCAACTTGAGGAGTGCCTGCAAGGCATGATGACGCATAAAGCGTCGGATCTGTATATCACTGTGGGCGCGCCCGTGTT
GTATCGGGTCGATGGTGAACTGCGCGCCCACGGCGACGCGCTGAATGAAGCGCAGGTCAGGACGCTGTTGGCCGCGATGA
TGGATGACACGCGCCATGCCGACTACTTGGCGACGCGTGAAGCCAACTTTGCCATTGTGCGTGATTTAGGGCGTTTTCGC
GTCAGTGCTTTTTTTCAGCGCGAGCTGCCCGGCGCAGTGATCCGCCGAATTGAAACGCAAATTCCCACGTTTGATGAGCT
CAAACTGCCACCCGTGTTGAAAGATCTCTCGATTGCCAAACGCGGTTTGGTGTTGGTGGTGGGGGCGACTGGATCGGGGA
AATCGACCACCATGGCCGCGATGACCGGGTATCGCAATATGCATCGCACCGGGCATATTCTCACCGTTGAAGATCCGATT
GAATTTGTCCATGAACATCAGCGCTGCATCGTGACGCAGCGCGAAGTGGGATTGGATACGGAAAGCTATGAAGTGGCGCT
GAAGAACTCGCTGCGTCAGGCGCCGGACATGATTTTGATCGGCGAGATCCGCAGTCGGGAAACCATGGAATACGCGATGA
CGTTTGCCGAAACGGGGCATTTGTGCATGGCCACTTTGCACGCCAACAACGCCAACCAAGCGCTTGAACGGATTTTACAT
CTGGTGCCGAAAGAGCAGCGTGAGCAATTTCTGTTTGATTTGTCGATGAACCTCAAAGGGGTTGTCGGCCAGCAACTGAT
CCGCGATAAAAACGGCCAAGGGCGTCACGGGGTGTTTGAAGTGCTGCTCAACAGCCCGCGGGTGTCGGACATCATTCGTC
GCGGCGAGCTGCATGAGTTGAAAAACACCATGGCGAAATCGAAAGAGGTTGGCATGCAGACTTTTGATCAGGCGCTGTAT
CAGTTGGTGGTTGAGCACAAGATCAGTGAGCAAGATGCGCTGCACAGTGCGGATTCGGCCAATGATCTGCGCTTGATGCT
CAAGATGCAGCGGGGCGATGTGACCAGCAGCGGGTCGCTGGATCATGTGCAAATCGACATGGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(73-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

85.598

100

0.856
  pilU Pseudomonas stutzeri DSM 10701

56.98

95.38

0.543
  pilU Acinetobacter baylyi ADP1

54.261

95.652

0.519
  pilT Acinetobacter baylyi ADP1

41.642

92.663

0.386
  pilT Legionella pneumophila strain Lp02

41.593

92.12

0.383
  pilT Legionella pneumophila strain ERS1305867

41.593

92.12

0.383
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.077

88.315

0.38
  pilT Acinetobacter baumannii D1279779

41.003

92.12

0.378
  pilT Acinetobacter baumannii strain A118

41.003

92.12

0.378
  pilT Acinetobacter nosocomialis M2

41.003

92.12

0.378
  pilT Pseudomonas aeruginosa PAK

40.708

92.12

0.375
  pilT Pseudomonas stutzeri DSM 10701

39.233

92.12

0.361