Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PY250_RS13665 Genome accession   NZ_CP119334
Coordinates   2915612..2917357 (-) Length   581 a.a.
NCBI ID   WP_042523674.1    Uniprot ID   A0A7Y0R021
Organism   Vibrio alginolyticus strain 2-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2910612..2922357
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PY250_RS13645 (PY250_13645) - 2911300..2912139 (-) 840 WP_005382762.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  PY250_RS13650 (PY250_13650) - 2912209..2913720 (-) 1512 WP_025768079.1 SPOR domain-containing protein -
  PY250_RS13655 (PY250_13655) aroB 2913773..2914873 (-) 1101 WP_170897642.1 3-dehydroquinate synthase -
  PY250_RS13660 (PY250_13660) aroK 2914906..2915424 (-) 519 WP_005381319.1 shikimate kinase AroK -
  PY250_RS13665 (PY250_13665) pilQ 2915612..2917357 (-) 1746 WP_042523674.1 type IV pilus secretin PilQ Machinery gene
  PY250_RS13670 (PY250_13670) pilP 2917383..2917898 (-) 516 WP_115386334.1 pilus assembly protein PilP Machinery gene
  PY250_RS13675 (PY250_13675) pilO 2917888..2918481 (-) 594 WP_033907305.1 type 4a pilus biogenesis protein PilO Machinery gene
  PY250_RS13680 (PY250_13680) pilN 2918474..2919049 (-) 576 WP_005381323.1 PilN domain-containing protein Machinery gene
  PY250_RS13685 (PY250_13685) pilM 2919033..2920049 (-) 1017 WP_170897641.1 type IV pilus biogenesis protein PilM Machinery gene

Sequence

Protein


Download         Length: 581 a.a.        Molecular weight: 63648.66 Da        Isoelectric Point: 4.4326

>NTDB_id=798560 PY250_RS13665 WP_042523674.1 2915612..2917357(-) (pilQ) [Vibrio alginolyticus strain 2-1]
MKMRQGLTRTPRILTGLFSLWLFVFLAPLAAAEEVSSNALKSIDFRTNKDKDAVIVVELASPAAIVDIKRVQEGLSIDLL
NTSVKDEQLYLLDVKDFSTVVESVEVFRDTSTTRLVAHINEEYMHDYRLTGPFLEIKVAKIKPNEKAPDKSILEREGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPTDELDLREKQQ
LEKQKLTEEMGELSSEIIKVNFAKASDIAEMINGEGNISMLSERGSMTIDERTNSLLIRELPENIEVIREIIESLDIPVK
QVQIEARIVTINEGNMDELGIRWGFTSINGSNTVGGSIENNLATIGLYNGEGGDDGGDGDGVGIDDFLNVNLAATSPNAT
SIAFQVAKLGSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATTVTFKKAVLSLKVTPQI
TPDNRLVLDLSVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGALFRR
SYENVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1746 bp        

>NTDB_id=798560 PY250_RS13665 WP_042523674.1 2915612..2917357(-) (pilQ) [Vibrio alginolyticus strain 2-1]
ATGAAAATGAGACAAGGATTAACACGTACACCAAGGATACTTACTGGGCTCTTCTCCTTATGGTTGTTTGTATTTTTAGC
CCCTTTAGCGGCAGCTGAAGAAGTTTCGTCCAATGCCCTAAAAAGTATCGACTTTCGAACTAATAAAGATAAAGACGCCG
TGATTGTCGTCGAATTGGCGTCACCGGCCGCGATTGTAGATATCAAGCGTGTACAGGAAGGGTTAAGTATCGATTTGCTT
AACACTTCAGTAAAAGACGAGCAATTGTATCTCCTAGACGTGAAAGACTTTTCGACTGTGGTTGAGAGCGTAGAAGTGTT
TCGCGATACCTCGACGACGCGCTTGGTTGCGCACATCAATGAAGAATACATGCATGATTACCGTTTAACGGGGCCTTTTT
TGGAAATCAAAGTAGCGAAGATCAAACCCAATGAGAAAGCCCCAGATAAAAGCATTTTAGAAAGAGAAGGTAAGCTTATT
TCGATTAACTTCCAAGATATTCCTGTGCGAAACGTCTTGCAGCTTATTGCTGATTACAACCAATTCAATTTGGTTGTCTC
CGACTCTGTAGAAGGCAACCTCACGCTTCGTCTTGATGGTGTCCCTTGGCAGCAAGTGCTGGATATTATTTTGCAAGTTA
AGGGGTTAGATAAGCGTGTTGATGGCAACGTGATTTTAGTTGCTCCGACTGATGAGTTGGACTTGCGTGAGAAGCAGCAG
TTAGAAAAACAAAAACTTACGGAAGAAATGGGAGAGCTTTCATCTGAAATCATCAAAGTTAATTTTGCTAAAGCATCAGA
CATTGCGGAGATGATCAATGGAGAAGGAAATATCAGCATGCTGTCAGAGCGCGGCAGTATGACCATTGATGAGCGAACTA
ACTCGTTATTGATTAGAGAGTTACCTGAAAACATCGAGGTGATTCGAGAAATTATTGAGTCTTTGGATATTCCAGTTAAA
CAGGTTCAAATTGAGGCGCGTATTGTGACCATCAATGAAGGTAACATGGACGAGCTAGGTATTCGTTGGGGTTTTACGTC
TATTAATGGCAGTAATACAGTGGGCGGTTCTATTGAGAACAACCTCGCCACGATCGGTCTTTATAATGGAGAAGGGGGCG
ACGACGGTGGTGACGGTGATGGAGTGGGGATCGATGACTTCCTAAATGTAAACTTAGCTGCAACCAGCCCGAATGCCACC
AGCATTGCTTTTCAGGTCGCAAAGTTAGGTTCAGACACCTTGTTGGATCTTGAGCTTTCTGCTCTGCAACAAGAGTCTAA
AGCTGAGATTATCTCGAGCCCACGTTTGATCACTACCAATAAGAAACCTGCCTATATCGAACAAGGTACTGAGATTCCTT
ATCTTGAGTCATCATCAAGTGGTGCAACGACGGTGACGTTCAAAAAGGCGGTATTAAGTTTGAAGGTAACCCCGCAAATC
ACACCGGATAACCGCTTAGTATTGGATTTAAGTGTCACGCAAGATAGACCTGGACAAGTCGTGAAGACAGGCACTGGTGA
AGCTGTTGCGATTGATACTCAACGTATCGGGACGCAAGTGTTAGTGAATAACGGTGAAACGGTCGTTCTTGGTGGTATTT
TTCAGCACAGCATTACTAACTCGGTGGACAAAGTGCCTTTGTTGGGCGATCTGCCGCTATTAGGTGCGCTTTTTCGTCGA
AGCTATGAAAATGTTGGCAAAAGTGAGCTGTTAATTTTCGTCACGCCTAAGGTGGTGATTCAGTAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(256-321)

Bacterial type II and III secretion system protein (Secretin)

(421-579)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Y0R021

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

92.771

100

0.928
  pilQ Vibrio cholerae strain A1552

74.742

100

0.749
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

74.742

100

0.749
  pilQ Pseudomonas aeruginosa PAK

39.415

100

0.394
  pilQ Legionella pneumophila strain Lp02

40.608

96.213

0.391
  pilQ Legionella pneumophila strain ERS1305867

40.608

96.213

0.391
  comQ Acinetobacter baylyi ADP1

37.414

99.828

0.373
  pilQ Acinetobacter baumannii D1279779

35.953

100

0.37
  pilQ Acinetobacter baumannii strain A118

35.953

100

0.37