Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   PY250_RS13685 Genome accession   NZ_CP119334
Coordinates   2919033..2920049 (-) Length   338 a.a.
NCBI ID   WP_170897641.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain 2-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2914033..2925049
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PY250_RS13660 (PY250_13660) aroK 2914906..2915424 (-) 519 WP_005381319.1 shikimate kinase AroK -
  PY250_RS13665 (PY250_13665) pilQ 2915612..2917357 (-) 1746 WP_042523674.1 type IV pilus secretin PilQ Machinery gene
  PY250_RS13670 (PY250_13670) pilP 2917383..2917898 (-) 516 WP_115386334.1 pilus assembly protein PilP Machinery gene
  PY250_RS13675 (PY250_13675) pilO 2917888..2918481 (-) 594 WP_033907305.1 type 4a pilus biogenesis protein PilO Machinery gene
  PY250_RS13680 (PY250_13680) pilN 2918474..2919049 (-) 576 WP_005381323.1 PilN domain-containing protein Machinery gene
  PY250_RS13685 (PY250_13685) pilM 2919033..2920049 (-) 1017 WP_170897641.1 type IV pilus biogenesis protein PilM Machinery gene
  PY250_RS13690 (PY250_13690) - 2920194..2922755 (+) 2562 WP_005381325.1 penicillin-binding protein 1A -
  PY250_RS13695 (PY250_13695) oxyR 2922809..2923720 (-) 912 WP_005381326.1 DNA-binding transcriptional regulator OxyR -
  PY250_RS13700 (PY250_13700) - 2923890..2924618 (+) 729 WP_005382781.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37541.91 Da        Isoelectric Point: 6.7707

>NTDB_id=798564 PY250_RS13685 WP_170897641.1 2919033..2920049(-) (pilM) [Vibrio alginolyticus strain 2-1]
MDKLIVTGIDIGHNSLKAVVLKPTGNNYALLGYKEILLKEGIVAENNTLNHQEIVKTLKQIKKELPFGARKVAISVPDNS
VISKKLQIEQNLEESEVEFAVIQAFSHQSPFPVEELSLDFVRLLAEGGQSGSDSYQVFATRKDVVEGRVDALQKSGLKPV
LVDVHSQSLGHIWKLTADRFPDKNKYCLLDIGTLSSSFTMFTEQGELFHKEFAYGTRGANSASPVDFLSDDAQEKTELFN
CQIVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLVEELSQQLSLECELLNPLGLFEMKVSKRKRRAVDWQHFSTAA
GLAVRGIHWLGGARALSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=798564 PY250_RS13685 WP_170897641.1 2919033..2920049(-) (pilM) [Vibrio alginolyticus strain 2-1]
ATGGATAAGCTAATCGTTACCGGCATCGATATAGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCACAGGCAACAA
TTACGCTCTGTTGGGTTACAAAGAAATATTACTAAAGGAAGGTATTGTCGCTGAAAATAACACCTTAAATCATCAAGAAA
TTGTAAAGACCCTCAAGCAGATAAAAAAAGAACTGCCTTTTGGCGCTAGAAAAGTTGCGATCTCAGTTCCTGATAACTCG
GTAATTAGCAAGAAACTGCAAATAGAACAAAATCTTGAAGAGAGTGAAGTTGAGTTTGCAGTGATACAAGCCTTTTCTCA
TCAATCGCCATTCCCTGTCGAAGAGCTGAGTTTAGACTTCGTTCGTCTTCTCGCGGAGGGCGGGCAGAGTGGCAGTGATA
GCTATCAAGTGTTTGCGACAAGAAAAGATGTGGTAGAAGGCCGAGTTGATGCATTACAAAAGTCCGGACTAAAACCTGTT
TTAGTGGACGTGCATTCGCAAAGCCTTGGTCATATCTGGAAGCTTACCGCCGATCGCTTTCCTGACAAAAACAAATACTG
CTTACTCGATATTGGCACCTTATCGAGCTCTTTCACCATGTTTACCGAACAAGGTGAACTGTTTCACAAAGAGTTTGCTT
ACGGCACACGCGGAGCAAACAGCGCTTCTCCAGTCGATTTTCTGTCCGATGATGCTCAGGAGAAAACAGAGCTGTTTAAC
TGTCAAATCGTAGAGCGAGTAAAGCGCCAGATGCAGCTCTATACCTCAATCAATGGCTCACAAAACATCAAGGGTATCTG
GTTATCAGGGGAGGGGGCCTCCACTCCCATGCTCGTAGAAGAGTTGTCTCAACAATTGTCGTTAGAGTGTGAATTGCTAA
ATCCGCTAGGGCTATTTGAGATGAAGGTGTCTAAGCGTAAGCGTCGTGCTGTGGATTGGCAGCATTTCTCAACAGCAGCC
GGACTGGCCGTTCGTGGCATTCATTGGCTAGGAGGCGCCCGTGCTTTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

82.544

100

0.825
  pilM Vibrio cholerae strain A1552

51.045

99.112

0.506