Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   PY250_RS13675 Genome accession   NZ_CP119334
Coordinates   2917888..2918481 (-) Length   197 a.a.
NCBI ID   WP_033907305.1    Uniprot ID   A0AAW9B9Y8
Organism   Vibrio alginolyticus strain 2-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2912888..2923481
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PY250_RS13655 (PY250_13655) aroB 2913773..2914873 (-) 1101 WP_170897642.1 3-dehydroquinate synthase -
  PY250_RS13660 (PY250_13660) aroK 2914906..2915424 (-) 519 WP_005381319.1 shikimate kinase AroK -
  PY250_RS13665 (PY250_13665) pilQ 2915612..2917357 (-) 1746 WP_042523674.1 type IV pilus secretin PilQ Machinery gene
  PY250_RS13670 (PY250_13670) pilP 2917383..2917898 (-) 516 WP_115386334.1 pilus assembly protein PilP Machinery gene
  PY250_RS13675 (PY250_13675) pilO 2917888..2918481 (-) 594 WP_033907305.1 type 4a pilus biogenesis protein PilO Machinery gene
  PY250_RS13680 (PY250_13680) pilN 2918474..2919049 (-) 576 WP_005381323.1 PilN domain-containing protein Machinery gene
  PY250_RS13685 (PY250_13685) pilM 2919033..2920049 (-) 1017 WP_170897641.1 type IV pilus biogenesis protein PilM Machinery gene
  PY250_RS13690 (PY250_13690) - 2920194..2922755 (+) 2562 WP_005381325.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23001.51 Da        Isoelectric Point: 5.0948

>NTDB_id=798562 PY250_RS13675 WP_033907305.1 2917888..2918481(-) (pilO) [Vibrio alginolyticus strain 2-1]
MVDWQDLEIDEMAEWPLLPQIVVIALLIMAIQGLGYWFYLKPKEEQLNHLIQQEQTLKVALKIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSEHFLYRLPLNIELTGDFHDIGHFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEETKNDK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=798562 PY250_RS13675 WP_033907305.1 2917888..2918481(-) (pilO) [Vibrio alginolyticus strain 2-1]
GTGGTTGATTGGCAGGATCTCGAAATAGATGAAATGGCCGAGTGGCCATTGTTGCCCCAGATTGTAGTAATCGCGCTTCT
GATCATGGCGATTCAAGGCTTAGGTTATTGGTTTTACCTTAAACCGAAAGAAGAACAGTTGAATCACTTGATCCAGCAGG
AGCAGACACTCAAGGTGGCATTGAAGATCAAAGCGAACAAAGTGGCAGCGTTGCCTCAGCTTCAATCTCAATTGGATGAA
CTTACCACTCGTTACGAGTTATTACTGCAACAACTGCCTGCGCAAAAAGAGCTGGCTACAATGCTCGCTTCAGTGAATGA
GCTTGGCATCGACAATAAGCTGACTTTCACCCGAATCGACTGGGGCGAAAAACAATCAGAGCACTTCCTTTATCGTCTTC
CTTTAAACATAGAGTTAACGGGCGACTTTCATGACATCGGCCATTTTTCTCAGGCAATAGCGACGCTACCTCGAATCATC
ACGTTTAAAGATGTGACTTGGCAACGTGTCAGCCAAGAGAGCGAAACCCTGCACTTTCGGGTGCGCGCTAACACCTATCA
GTTTAAGCCGGAGGAGACGAAAAATGACAAGTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(48-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

87.895

96.447

0.848
  pilO Vibrio cholerae strain A1552

70.681

96.954

0.685