Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PWW26_RS02040 Genome accession   NZ_CP118596
Coordinates   422698..424449 (+) Length   583 a.a.
NCBI ID   WP_274879041.1    Uniprot ID   -
Organism   Vibrio harveyi strain fish10     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 417698..429449
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWW26_RS02020 pilM 419997..421013 (+) 1017 WP_005451497.1 type IV pilus assembly protein PilM Machinery gene
  PWW26_RS02025 pilN 420997..421572 (+) 576 WP_005451499.1 PilN domain-containing protein Machinery gene
  PWW26_RS02030 pilO 421565..422158 (+) 594 WP_005451500.1 type 4a pilus biogenesis protein PilO Machinery gene
  PWW26_RS02035 pilP 422148..422663 (+) 516 WP_274878485.1 pilus assembly protein PilP Machinery gene
  PWW26_RS02040 pilQ 422698..424449 (+) 1752 WP_274879041.1 type IV pilus secretin PilQ family protein Machinery gene
  PWW26_RS02045 aroK 424639..425157 (+) 519 WP_005451507.1 shikimate kinase AroK -
  PWW26_RS02050 aroB 425189..426289 (+) 1101 WP_038898114.1 3-dehydroquinate synthase -
  PWW26_RS02055 - 426343..427845 (+) 1503 WP_274878487.1 AAA family ATPase -
  PWW26_RS02060 - 427915..428754 (+) 840 WP_239912440.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 583 a.a.        Molecular weight: 63782.82 Da        Isoelectric Point: 4.5077

>NTDB_id=793786 PWW26_RS02040 WP_274879041.1 422698..424449(+) (pilQ) [Vibrio harveyi strain fish10]
MKMRQGLTLTPRKLTGLFSLWLFLLLAPLVAAEEVKSNSLKSIDFRTNKDKDAVIVVELASPAAIVDIKRVQEGLSIDLL
NTSVKDDQLYLLDVKDFSTVVESVEVFRDTSTTRLVAYIDEDYTHDYRLTGRYLEVKVGKLKANEKAPDKSILEKEGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKDELDLREKQQ
LEKQKLEQEMGELSSEIIKVNFAKASDIAEMINGEGNISMLSERGSMTIDERTNSLLIRELPENIQVIREIIESLDIPVK
QVQIEARIVTINEGNMDELGVRWGFTSINGSHTVGGSIENNLATIGLYDGGGGDGDGEDGGGSVGIDDFLNVNLAATSPN
ATSIAFQVAKLGSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVTFKKAVLSLKVTP
QITPDNRLVLDLSVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGALF
RRSYENVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1752 bp        

>NTDB_id=793786 PWW26_RS02040 WP_274879041.1 422698..424449(+) (pilQ) [Vibrio harveyi strain fish10]
ATGAAAATGAGACAAGGATTAACACTGACACCAAGGAAGCTAACAGGGCTCTTCTCTTTATGGTTGTTTCTACTTTTAGC
CCCTTTAGTCGCAGCGGAAGAAGTAAAGTCTAACTCGCTTAAGAGCATCGACTTCCGTACTAATAAAGATAAAGACGCGG
TTATCGTCGTAGAGCTTGCATCGCCAGCTGCTATTGTTGATATCAAGCGTGTGCAAGAAGGTTTAAGTATCGACTTGCTC
AATACGTCAGTTAAAGACGATCAGCTTTATCTACTTGATGTAAAAGACTTCTCTACAGTGGTAGAGAGTGTTGAGGTTTT
CCGCGATACCTCTACGACGCGTTTGGTCGCTTACATTGATGAAGATTATACCCATGACTATCGCCTCACAGGGCGTTATC
TAGAGGTGAAGGTTGGTAAGCTCAAAGCCAACGAAAAAGCACCAGACAAAAGTATTCTGGAAAAAGAAGGCAAGTTGATT
TCGATTAACTTCCAAGATATTCCGGTGCGAAATGTGCTGCAGCTTATTGCTGATTACAACCAGTTTAACCTTGTGGTTTC
AGACTCAGTTGAAGGGAACCTGACCTTGCGTTTGGACGGTGTACCTTGGCAACAAGTGCTGGATATCATCCTTCAAGTCA
AAGGCTTGGATAAGCGTGTAGATGGTAACGTTATTTTGGTTGCGCCAAAGGATGAGCTCGATCTGCGCGAGAAGCAACAA
CTAGAAAAACAAAAGCTTGAACAAGAAATGGGTGAGCTTTCGTCTGAAATTATCAAAGTGAATTTTGCGAAAGCATCGGA
TATTGCAGAGATGATTAACGGTGAAGGTAACATTAGTATGCTTTCTGAACGTGGCAGCATGACGATTGATGAGCGTACTA
ACTCATTATTGATCCGTGAGTTGCCGGAGAATATTCAAGTAATTCGCGAAATCATTGAATCATTAGATATCCCAGTAAAA
CAAGTTCAGATTGAAGCACGTATCGTGACTATCAATGAAGGTAACATGGATGAACTTGGCGTTCGCTGGGGCTTCACATC
GATTAATGGTAGTCATACGGTTGGTGGATCGATCGAGAACAACCTTGCAACTATTGGTCTCTACGATGGCGGCGGTGGCG
ACGGCGATGGTGAAGACGGTGGCGGCAGTGTTGGTATTGATGACTTCCTGAACGTTAACCTTGCTGCAACCAGCCCGAAT
GCAACCAGTATTGCTTTCCAAGTTGCGAAACTAGGGTCTGATACTTTGTTGGATTTGGAGTTGTCTGCATTGCAACAAGA
ATCAAAAGCAGAAATCATTTCTAGTCCGCGTTTAATTACTACCAATAAGAAGCCAGCTTATATTGAACAAGGTACTGAAA
TTCCTTACCTAGAATCCTCTTCCAGCGGTGCGACCTCCGTAACGTTCAAAAAAGCAGTGCTGAGCTTAAAAGTGACACCG
CAGATCACGCCTGATAACCGTTTGGTGCTGGATTTGAGTGTTACGCAAGATAGACCGGGTCAAGTGGTAAAAACTGGTAC
CGGTGAGGCAGTTGCGATTGATACTCAGCGTATTGGCACTCAAGTATTGGTGAATAATGGTGAGACGGTTGTGCTTGGTG
GTATTTTCCAACACAGCATTACAAACTCCGTCGATAAGGTACCTTTATTAGGTGATTTACCGCTGTTAGGGGCACTTTTC
CGTCGAAGTTACGAAAATGTCGGTAAAAGTGAGTTGCTGATTTTTGTGACGCCTAAAGTGGTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(256-321)

Bacterial type II and III secretion system protein (Secretin)

(423-581)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

96.913

100

0.969
  pilQ Vibrio cholerae strain A1552

77.504

97.599

0.756
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

77.504

97.599

0.756
  pilQ Pseudomonas aeruginosa PAK

40.138

99.142

0.398
  pilQ Legionella pneumophila strain ERS1305867

41.091

94.34

0.388
  pilQ Legionella pneumophila strain Lp02

41.091

94.34

0.388
  pilQ Acinetobacter baumannii D1279779

36.833

100

0.379
  pilQ Acinetobacter baumannii strain A118

36.833

100

0.379
  comQ Acinetobacter baylyi ADP1

36.986

100

0.37