Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PWW22_RS13560 Genome accession   NZ_CP118578
Coordinates   2977603..2979354 (-) Length   583 a.a.
NCBI ID   WP_274881159.1    Uniprot ID   -
Organism   Vibrio harveyi strain fish2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2972603..2984354
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWW22_RS13540 - 2973298..2974137 (-) 840 WP_005451513.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  PWW22_RS13545 - 2974207..2975709 (-) 1503 WP_005451510.1 AAA family ATPase -
  PWW22_RS13550 aroB 2975763..2976863 (-) 1101 WP_038898114.1 3-dehydroquinate synthase -
  PWW22_RS13555 aroK 2976895..2977413 (-) 519 WP_005451507.1 shikimate kinase AroK -
  PWW22_RS13560 pilQ 2977603..2979354 (-) 1752 WP_274881159.1 type IV pilus secretin PilQ family protein Machinery gene
  PWW22_RS13565 pilP 2979389..2979904 (-) 516 WP_274880475.1 pilus assembly protein PilP Machinery gene
  PWW22_RS13570 pilO 2979894..2980487 (-) 594 WP_005451500.1 type 4a pilus biogenesis protein PilO Machinery gene
  PWW22_RS13575 pilN 2980480..2981055 (-) 576 WP_005451499.1 PilN domain-containing protein Machinery gene
  PWW22_RS13580 pilM 2981039..2982055 (-) 1017 WP_026138410.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 583 a.a.        Molecular weight: 63782.82 Da        Isoelectric Point: 4.5053

>NTDB_id=793530 PWW22_RS13560 WP_274881159.1 2977603..2979354(-) (pilQ) [Vibrio harveyi strain fish2]
MKMRQGLTLTPRKLTGLFSLWLFLLLAPLVAAEEVKSNSLKSIDFRTNKDKDAVIVVELASPAAIVDIKRVQEGLSIDLL
NTSIKDDQLYLLDVKDFSTVVESVEVFRDTSTTRLVAYIDEDYTHDYRLTGRYLEVKVGKLKANEKAPDKSILEKEGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKDELDLREKQQ
LEKQKLEQEMGELSSEIIKVNFAKASDIAEMINGEGNISMLSDRGSMTIDERTNSLLIRELPENIQVIREIIESLDIPVK
QVQIEARIVTINEGNMDELGVRWGFTSINGSHTVGGSIENNLATIGLYDGGGGDGDGEDGGGSVGIDDFLNVNLAATSPN
ATSIAFQVAKLGSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVTFKKAVLSLKVTP
QITPDNRLVLDLSVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGALF
RRSYENVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1752 bp        

>NTDB_id=793530 PWW22_RS13560 WP_274881159.1 2977603..2979354(-) (pilQ) [Vibrio harveyi strain fish2]
ATGAAAATGAGACAAGGATTAACACTGACACCAAGGAAGCTAACAGGGCTCTTCTCTTTATGGTTGTTTCTACTTTTAGC
CCCTTTAGTCGCAGCGGAAGAAGTAAAGTCTAACTCGCTTAAGAGCATCGACTTCCGTACTAATAAAGATAAAGACGCGG
TTATCGTCGTAGAGCTTGCATCGCCAGCTGCTATTGTTGATATCAAGCGTGTGCAAGAAGGTTTAAGTATCGACTTGCTC
AATACGTCAATTAAAGACGATCAGCTTTATCTACTTGATGTAAAAGACTTCTCTACAGTGGTAGAGAGTGTTGAGGTTTT
CCGCGATACCTCTACGACGCGTTTGGTCGCTTACATTGATGAAGATTATACCCATGACTATCGCCTCACAGGGCGTTATC
TAGAGGTGAAGGTTGGTAAGCTCAAAGCCAACGAAAAAGCACCAGACAAAAGTATTCTGGAAAAAGAAGGCAAGTTGATT
TCGATTAACTTCCAAGATATTCCGGTGCGAAATGTGCTGCAGCTTATTGCTGATTACAACCAGTTTAACCTTGTGGTTTC
AGACTCAGTTGAAGGGAACCTGACCTTGCGTTTGGACGGTGTACCTTGGCAACAAGTGCTGGATATCATCCTTCAAGTCA
AAGGCTTGGATAAGCGTGTAGATGGTAACGTTATTTTGGTTGCTCCGAAGGATGAGCTCGATCTGCGCGAGAAGCAACAA
CTAGAAAAACAAAAGCTTGAACAAGAAATGGGTGAACTTTCTTCTGAAATTATCAAAGTGAATTTTGCGAAAGCATCGGA
TATTGCAGAGATGATTAACGGTGAAGGTAACATTAGTATGCTTTCTGATCGTGGCAGCATGACGATTGATGAGCGTACTA
ACTCATTATTGATCCGTGAGTTGCCGGAGAATATTCAAGTAATTCGTGAAATCATTGAATCATTAGATATTCCAGTAAAA
CAAGTTCAGATTGAAGCACGTATCGTGACTATCAATGAAGGTAACATGGATGAACTTGGCGTTCGCTGGGGCTTCACATC
GATTAATGGTAGTCATACGGTTGGTGGATCGATCGAGAACAACCTTGCAACTATTGGTCTCTACGATGGCGGCGGTGGCG
ACGGCGATGGTGAAGACGGTGGCGGCAGTGTTGGTATTGATGACTTCCTGAACGTTAACCTTGCTGCAACCAGCCCAAAT
GCAACCAGTATTGCTTTCCAAGTTGCGAAACTAGGGTCTGATACTTTGTTGGATTTGGAGTTGTCTGCGTTGCAACAAGA
ATCAAAAGCAGAAATCATTTCTAGTCCGCGTTTAATTACTACCAATAAGAAGCCAGCTTATATTGAACAAGGTACTGAAA
TTCCTTACCTAGAATCCTCTTCCAGCGGTGCGACATCCGTAACGTTCAAAAAAGCGGTGCTGAGCTTAAAAGTGACACCG
CAGATCACGCCTGATAACCGTTTGGTGCTGGATTTGAGTGTTACGCAAGATAGACCGGGTCAAGTGGTAAAAACTGGTAC
CGGTGAGGCAGTTGCGATTGATACTCAGCGTATTGGCACTCAAGTATTGGTGAATAATGGTGAGACGGTTGTGCTTGGTG
GTATTTTCCAACACAGCATTACAAACTCCGTCGATAAGGTACCTTTATTAGGTGATTTACCGCTGTTAGGGGCACTTTTC
CGTCGAAGTTACGAAAATGTCGGTAAAAGTGAGTTGCTGATTTTTGTTACGCCTAAAGTGGTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(423-581)

Bacterial type II/III secretion system short domain (Secretin_N)

(256-321)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

96.913

100

0.969
  pilQ Vibrio cholerae strain A1552

77.153

97.599

0.753
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

77.153

97.599

0.753
  pilQ Pseudomonas aeruginosa PAK

40.138

99.142

0.398
  pilQ Legionella pneumophila strain ERS1305867

41.455

94.34

0.391
  pilQ Legionella pneumophila strain Lp02

41.455

94.34

0.391
  pilQ Acinetobacter baumannii D1279779

37

100

0.381
  pilQ Acinetobacter baumannii strain A118

37

100

0.381
  comQ Acinetobacter baylyi ADP1

37.158

100

0.372