Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   PWW22_RS13580 Genome accession   NZ_CP118578
Coordinates   2981039..2982055 (-) Length   338 a.a.
NCBI ID   WP_026138410.1    Uniprot ID   -
Organism   Vibrio harveyi strain fish2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2976039..2987055
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWW22_RS13555 aroK 2976895..2977413 (-) 519 WP_005451507.1 shikimate kinase AroK -
  PWW22_RS13560 pilQ 2977603..2979354 (-) 1752 WP_274881159.1 type IV pilus secretin PilQ family protein Machinery gene
  PWW22_RS13565 pilP 2979389..2979904 (-) 516 WP_274880475.1 pilus assembly protein PilP Machinery gene
  PWW22_RS13570 pilO 2979894..2980487 (-) 594 WP_005451500.1 type 4a pilus biogenesis protein PilO Machinery gene
  PWW22_RS13575 pilN 2980480..2981055 (-) 576 WP_005451499.1 PilN domain-containing protein Machinery gene
  PWW22_RS13580 pilM 2981039..2982055 (-) 1017 WP_026138410.1 type IV pilus assembly protein PilM Machinery gene
  PWW22_RS13585 - 2982234..2984804 (+) 2571 WP_050935720.1 PBP1A family penicillin-binding protein -
  PWW22_RS13590 oxyR 2984871..2985779 (-) 909 WP_005451495.1 DNA-binding transcriptional regulator OxyR -
  PWW22_RS13595 - 2985954..2986682 (+) 729 WP_005451494.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38053.43 Da        Isoelectric Point: 6.4206

>NTDB_id=793534 PWW22_RS13580 WP_026138410.1 2981039..2982055(-) (pilM) [Vibrio harveyi strain fish2]
MDKLLVTGIDIGHNSLKAVVLKPNGNSYALLGYKEIILKEGIVAENNTINHQEIVKTLKEIRKELPFWARRVAISVPDNA
VISKKLQIEQNLEESEKEFAVVQAFSHQSPFPVEELSLDFVKLDADEQMRGTDSYQVFATRKDVVETRVDALQKSGLKTV
LVDVHSQSLGHIWKLAVERFPEKSKYCLLDISALSSSFTMFTEQSELFHKEFPYGTRTSNDLFQQDPLAEDTQVATEQFN
RQIVDRVKRQIQLYTSINGSQDIKGIWLSGEGAATPMLAEELSSQLALECELLNPLGLFEMKVSKRKRRAADWQHFTAAA
GLAVRGIQWLGGKHAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=793534 PWW22_RS13580 WP_026138410.1 2981039..2982055(-) (pilM) [Vibrio harveyi strain fish2]
ATGGATAAGCTATTAGTTACGGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTGGTACTTAAACCCAATGGCAACAG
TTACGCCTTATTGGGTTACAAAGAAATAATACTTAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTTAAAGAGATAAGAAAAGAGCTGCCATTTTGGGCTCGACGGGTCGCAATTTCTGTTCCTGATAATGCC
GTGATCAGCAAGAAGCTGCAAATTGAGCAGAACTTGGAGGAGAGTGAGAAAGAGTTTGCCGTGGTGCAAGCTTTCTCGCA
TCAATCCCCATTTCCTGTTGAAGAGCTCAGTCTTGATTTTGTAAAGCTTGATGCCGATGAGCAGATGCGTGGAACAGACA
GTTATCAAGTATTCGCGACTCGCAAAGATGTCGTTGAAACTCGTGTCGATGCTCTGCAAAAGTCAGGCTTGAAAACCGTG
CTGGTGGACGTGCATTCACAAAGTTTAGGGCATATATGGAAACTGGCTGTCGAGCGCTTCCCTGAAAAGAGCAAGTATTG
CTTGTTGGATATCAGTGCATTGAGCAGCTCTTTCACCATGTTTACAGAGCAGAGTGAATTGTTCCACAAGGAATTTCCTT
ACGGCACGCGCACTTCAAATGATCTATTTCAGCAAGACCCTCTTGCTGAAGACACGCAAGTTGCTACTGAGCAGTTTAAT
CGACAAATCGTTGATCGTGTAAAGCGCCAGATTCAGCTCTATACCTCGATTAATGGAAGTCAAGACATCAAAGGCATTTG
GTTGTCTGGTGAAGGTGCAGCTACGCCAATGTTAGCAGAAGAACTTTCAAGTCAGTTGGCATTGGAGTGTGAGTTGCTTA
ATCCGCTCGGTCTATTCGAGATGAAAGTATCGAAACGCAAACGCCGTGCAGCAGATTGGCAGCATTTTACTGCGGCTGCG
GGCTTAGCGGTTAGAGGTATTCAATGGCTAGGAGGCAAACATGCTGTATCACATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

90.828

100

0.908
  pilM Vibrio cholerae strain A1552

48.83

100

0.494