Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PUT85_RS01295 Genome accession   NZ_CP118530
Coordinates   270269..272026 (+) Length   585 a.a.
NCBI ID   WP_195724146.1    Uniprot ID   -
Organism   Vibrio harveyi strain A2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 265269..277026
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUT85_RS01275 pilM 267568..268584 (+) 1017 WP_026138410.1 type IV pilus assembly protein PilM Machinery gene
  PUT85_RS01280 pilN 268568..269143 (+) 576 WP_005451499.1 PilN domain-containing protein Machinery gene
  PUT85_RS01285 pilO 269136..269729 (+) 594 WP_005451500.1 type 4a pilus biogenesis protein PilO Machinery gene
  PUT85_RS01290 pilP 269719..270234 (+) 516 WP_005451501.1 pilus assembly protein PilP Machinery gene
  PUT85_RS01295 pilQ 270269..272026 (+) 1758 WP_195724146.1 type IV pilus secretin PilQ family protein Machinery gene
  PUT85_RS01300 aroK 272216..272734 (+) 519 WP_005451507.1 shikimate kinase AroK -
  PUT85_RS01305 aroB 272766..273866 (+) 1101 WP_274790510.1 3-dehydroquinate synthase -
  PUT85_RS01310 - 273920..275422 (+) 1503 WP_053050710.1 AAA family ATPase -
  PUT85_RS01315 - 275492..276331 (+) 840 WP_005451513.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 585 a.a.        Molecular weight: 63940.93 Da        Isoelectric Point: 4.4829

>NTDB_id=792915 PUT85_RS01295 WP_195724146.1 270269..272026(+) (pilQ) [Vibrio harveyi strain A2]
MKMRQGLTLTPRKLTGLFSLWLFLLLAPLVAAEEVKSNSLKSIDFRTNKDKDAVIVVELASPAAIVDIKRVQEGLSIDLL
NTSVKDDQLYLLDVKDFSTVVESVEVFRDTSTTRLVAYIDEDYTHDYRLTGRYLEVKVGKLKANEKAPDKSILEKEGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKDELDLREKQQ
LEKQKLEQEMGELSSEIIKVNFAKASDIAEMINGEGNISMLSDRGSMTIDERTNSLLIRELPENIQVIREIIESLDIPVK
QVQIEARIVTINEGNMDELGVRWGFTSINGSHTVGGSIENNLATIGLYDGGGGDGDGDGEDGGGSVGIDDFLNVNLAATS
PNATSIAFQVAKLGSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVTFKKAVLSLKV
TPQITPDNRLVLDLSVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGA
LFRRSYENVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1758 bp        

>NTDB_id=792915 PUT85_RS01295 WP_195724146.1 270269..272026(+) (pilQ) [Vibrio harveyi strain A2]
ATGAAAATGAGACAAGGATTAACACTGACACCAAGGAAGCTAACAGGGCTCTTCTCTTTATGGTTGTTTCTACTTTTAGC
CCCTTTAGTCGCAGCGGAAGAAGTAAAGTCTAACTCGCTTAAGAGCATCGACTTCCGTACTAATAAAGATAAAGACGCGG
TTATCGTCGTAGAGCTTGCATCGCCAGCTGCTATTGTTGATATCAAGCGTGTGCAAGAAGGTTTAAGTATCGACTTGCTC
AATACGTCAGTTAAAGACGATCAGCTTTATCTACTTGATGTAAAAGACTTCTCTACAGTGGTAGAGAGTGTTGAGGTTTT
CCGCGATACCTCTACGACGCGTTTGGTCGCTTACATTGATGAAGATTATACCCATGACTATCGCCTCACAGGGCGTTATC
TAGAGGTGAAGGTTGGTAAGCTCAAAGCCAACGAAAAAGCACCAGACAAAAGTATTCTGGAAAAAGAAGGCAAGTTGATT
TCGATTAACTTCCAAGATATTCCGGTGCGAAATGTGCTGCAGCTTATTGCTGATTACAACCAGTTTAACCTTGTGGTTTC
AGACTCAGTTGAAGGGAACCTGACCTTGCGTTTGGACGGTGTACCTTGGCAACAAGTGCTGGATATCATCCTTCAAGTCA
AAGGCTTGGATAAGCGTGTAGATGGTAACGTTATTTTGGTTGCTCCGAAGGATGAGCTCGATCTGCGCGAGAAGCAACAA
CTAGAAAAACAAAAGCTTGAACAAGAAATGGGTGAACTTTCTTCTGAAATTATCAAAGTGAATTTTGCGAAAGCATCGGA
TATTGCAGAGATGATTAACGGTGAAGGTAACATTAGTATGCTTTCTGATCGTGGCAGCATGACGATTGATGAGCGTACTA
ACTCATTATTGATCCGTGAGTTGCCGGAGAATATTCAAGTAATTCGTGAAATCATTGAATCATTAGATATTCCAGTAAAA
CAAGTTCAGATTGAAGCACGTATCGTGACTATCAATGAAGGTAACATGGATGAACTTGGCGTTCGCTGGGGCTTCACATC
GATTAATGGTAGTCATACGGTTGGTGGATCGATCGAGAACAACCTTGCAACTATTGGTCTCTACGATGGCGGCGGTGGCG
ACGGCGATGGCGATGGCGAAGACGGTGGCGGCAGTGTTGGTATTGATGACTTCCTGAACGTTAACCTTGCTGCAACCAGC
CCTAATGCAACCAGTATTGCTTTCCAAGTTGCAAAACTAGGGTCTGATACTTTGTTGGATTTGGAGTTGTCTGCGTTGCA
ACAAGAATCAAAAGCAGAAATCATTTCTAGTCCGCGTTTAATTACTACCAATAAGAAGCCAGCTTATATTGAACAAGGTA
CAGAAATTCCTTACCTAGAATCCTCTTCCAGCGGTGCGACATCCGTAACGTTCAAAAAAGCGGTGCTGAGCTTAAAAGTG
ACACCGCAGATCACGCCTGATAACCGTTTGGTACTGGATTTGAGTGTTACGCAAGATAGACCGGGTCAAGTGGTAAAAAC
TGGTACCGGTGAGGCAGTTGCGATTGATACTCAGCGTATTGGCACTCAAGTATTGGTGAATAATGGTGAGACGGTTGTGC
TTGGTGGTATTTTCCAACACAGCATTACAAACTCCGTCGATAAGGTACCTTTATTAGGTGATTTACCGCTGTTAGGGGCA
CTTTTCCGTCGAAGTTACGAAAATGTCGGTAAAAGTGAGTTGCTGATTTTTGTTACGCCTAAAGTGGTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(425-583)

Bacterial type II/III secretion system short domain (Secretin_N)

(256-321)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

96.752

100

0.968
  pilQ Vibrio cholerae strain A1552

77.058

97.607

0.752
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

77.058

97.607

0.752
  pilQ Pseudomonas aeruginosa PAK

40.172

99.145

0.398
  pilQ Legionella pneumophila strain ERS1305867

41.123

94.359

0.388
  pilQ Legionella pneumophila strain Lp02

41.123

94.359

0.388
  pilQ Acinetobacter baumannii D1279779

36.877

100

0.379
  pilQ Acinetobacter baumannii strain A118

36.877

100

0.379
  comQ Acinetobacter baylyi ADP1

36.86

100

0.369