Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   PUT85_RS01285 Genome accession   NZ_CP118530
Coordinates   269136..269729 (+) Length   197 a.a.
NCBI ID   WP_005451500.1    Uniprot ID   A0A9X3S2A2
Organism   Vibrio harveyi strain A2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 264136..274729
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUT85_RS01270 - 264819..267389 (-) 2571 WP_050935720.1 PBP1A family penicillin-binding protein -
  PUT85_RS01275 pilM 267568..268584 (+) 1017 WP_026138410.1 type IV pilus assembly protein PilM Machinery gene
  PUT85_RS01280 pilN 268568..269143 (+) 576 WP_005451499.1 PilN domain-containing protein Machinery gene
  PUT85_RS01285 pilO 269136..269729 (+) 594 WP_005451500.1 type 4a pilus biogenesis protein PilO Machinery gene
  PUT85_RS01290 pilP 269719..270234 (+) 516 WP_005451501.1 pilus assembly protein PilP Machinery gene
  PUT85_RS01295 pilQ 270269..272026 (+) 1758 WP_195724146.1 type IV pilus secretin PilQ family protein Machinery gene
  PUT85_RS01300 aroK 272216..272734 (+) 519 WP_005451507.1 shikimate kinase AroK -
  PUT85_RS01305 aroB 272766..273866 (+) 1101 WP_274790510.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23020.58 Da        Isoelectric Point: 4.8068

>NTDB_id=792913 PUT85_RS01285 WP_005451500.1 269136..269729(+) (pilO) [Vibrio harveyi strain A2]
MVNWQDLEMDEIAEWPLVPQIVVILLLVVLIQGLGYWFYLKPEQEQLNNLIQEEQTLKVALRIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGDKQSEHFLYRLPLDIELTGDFHDIGRFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVKANTYQFKPEVKKDEK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=792913 PUT85_RS01285 WP_005451500.1 269136..269729(+) (pilO) [Vibrio harveyi strain A2]
ATGGTTAATTGGCAAGATCTAGAAATGGATGAGATCGCTGAGTGGCCATTGGTGCCTCAGATAGTAGTGATCTTGTTGTT
AGTTGTGCTGATTCAAGGCCTTGGTTATTGGTTTTATTTAAAGCCAGAGCAAGAACAGCTGAATAACCTTATTCAAGAAG
AACAAACCTTAAAAGTTGCGTTACGGATCAAAGCGAACAAAGTCGCTGCTTTGCCTCAATTGCAATCGCAACTTGATGAG
CTTACTACTCGATACGAATTGCTACTGCAACAACTGCCTGCACAGAAAGAGCTGGCAACCATGTTGGCTTCGGTGAATGA
ACTCGGAATTGATAATAAGTTGACGTTCACCCGAATCGACTGGGGTGATAAGCAATCAGAACACTTCCTTTATCGTTTAC
CGCTCGACATTGAGTTGACAGGGGATTTTCATGATATCGGTCGTTTCTCCCAAGCGATTGCGACGTTGCCACGCATCATC
ACTTTTAAAGACGTGACTTGGCAGCGTGTCAGCCAAGAGAGTGAAACTCTGCACTTTCGTGTGAAAGCAAACACTTACCA
GTTCAAACCAGAGGTGAAGAAAGATGAGAAGTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

93.158

96.447

0.898
  pilO Vibrio cholerae strain A1552

71.066

100

0.711