Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   PUT85_RS01275 Genome accession   NZ_CP118530
Coordinates   267568..268584 (+) Length   338 a.a.
NCBI ID   WP_026138410.1    Uniprot ID   -
Organism   Vibrio harveyi strain A2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 262568..273584
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUT85_RS01260 - 262941..263669 (-) 729 WP_005451494.1 redoxin family protein -
  PUT85_RS01265 oxyR 263844..264752 (+) 909 WP_005451495.1 DNA-binding transcriptional regulator OxyR -
  PUT85_RS01270 - 264819..267389 (-) 2571 WP_050935720.1 PBP1A family penicillin-binding protein -
  PUT85_RS01275 pilM 267568..268584 (+) 1017 WP_026138410.1 type IV pilus assembly protein PilM Machinery gene
  PUT85_RS01280 pilN 268568..269143 (+) 576 WP_005451499.1 PilN domain-containing protein Machinery gene
  PUT85_RS01285 pilO 269136..269729 (+) 594 WP_005451500.1 type 4a pilus biogenesis protein PilO Machinery gene
  PUT85_RS01290 pilP 269719..270234 (+) 516 WP_005451501.1 pilus assembly protein PilP Machinery gene
  PUT85_RS01295 pilQ 270269..272026 (+) 1758 WP_195724146.1 type IV pilus secretin PilQ family protein Machinery gene
  PUT85_RS01300 aroK 272216..272734 (+) 519 WP_005451507.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38053.43 Da        Isoelectric Point: 6.4206

>NTDB_id=792911 PUT85_RS01275 WP_026138410.1 267568..268584(+) (pilM) [Vibrio harveyi strain A2]
MDKLLVTGIDIGHNSLKAVVLKPNGNSYALLGYKEIILKEGIVAENNTINHQEIVKTLKEIRKELPFWARRVAISVPDNA
VISKKLQIEQNLEESEKEFAVVQAFSHQSPFPVEELSLDFVKLDADEQMRGTDSYQVFATRKDVVETRVDALQKSGLKTV
LVDVHSQSLGHIWKLAVERFPEKSKYCLLDISALSSSFTMFTEQSELFHKEFPYGTRTSNDLFQQDPLAEDTQVATEQFN
RQIVDRVKRQIQLYTSINGSQDIKGIWLSGEGAATPMLAEELSSQLALECELLNPLGLFEMKVSKRKRRAADWQHFTAAA
GLAVRGIQWLGGKHAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=792911 PUT85_RS01275 WP_026138410.1 267568..268584(+) (pilM) [Vibrio harveyi strain A2]
ATGGATAAGCTATTAGTTACGGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTGGTACTTAAACCCAATGGCAACAG
TTACGCCTTATTGGGTTACAAAGAAATAATACTTAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTTAAAGAGATAAGAAAAGAGCTGCCATTTTGGGCTCGACGGGTCGCAATTTCTGTTCCTGATAATGCC
GTGATCAGCAAGAAGCTGCAAATTGAGCAGAACTTGGAGGAGAGTGAGAAAGAGTTTGCCGTGGTGCAAGCTTTCTCGCA
TCAATCCCCATTTCCTGTTGAAGAGCTCAGTCTTGATTTTGTAAAGCTTGATGCCGATGAGCAGATGCGTGGAACAGACA
GTTATCAAGTATTCGCGACTCGCAAAGATGTCGTTGAAACTCGTGTCGATGCTCTGCAAAAGTCAGGCTTGAAAACCGTG
CTGGTGGACGTGCATTCACAAAGTTTAGGGCATATATGGAAACTGGCTGTCGAGCGCTTCCCTGAAAAGAGCAAGTATTG
CTTGTTGGATATCAGTGCATTGAGCAGCTCTTTCACCATGTTTACAGAGCAGAGTGAATTGTTCCACAAGGAATTTCCTT
ACGGCACGCGCACTTCAAATGATCTATTTCAGCAAGACCCTCTTGCTGAAGACACGCAGGTTGCTACCGAGCAGTTTAAT
CGACAAATCGTTGATCGTGTAAAGCGCCAGATTCAGCTCTATACCTCGATTAATGGAAGTCAAGACATCAAAGGCATTTG
GTTGTCTGGTGAAGGTGCAGCTACGCCAATGTTAGCAGAAGAACTTTCAAGTCAGTTGGCATTGGAGTGTGAGTTGCTTA
ATCCGCTCGGTCTATTCGAGATGAAAGTATCGAAACGCAAACGCCGTGCAGCAGATTGGCAGCATTTTACTGCGGCTGCG
GGCTTAGCGGTTAGAGGTATTCAATGGCTAGGAGGCAAACATGCTGTATCACATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

90.828

100

0.908
  pilM Vibrio cholerae strain A1552

48.83

100

0.494