Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PUN50_RS01585 Genome accession   NZ_CP117988
Coordinates   333385..335133 (+) Length   582 a.a.
NCBI ID   WP_045369316.1    Uniprot ID   A0AAQ2Y0E1
Organism   Vibrio campbellii strain 20220413_1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 328385..340133
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUN50_RS01565 (PUN50_01565) pilM 330684..331700 (+) 1017 WP_274290764.1 type IV pilus biogenesis protein PilM Machinery gene
  PUN50_RS01570 (PUN50_01570) pilN 331684..332259 (+) 576 WP_005432710.1 PilN domain-containing protein Machinery gene
  PUN50_RS01575 (PUN50_01575) pilO 332252..332845 (+) 594 WP_005432718.1 type 4a pilus biogenesis protein PilO Machinery gene
  PUN50_RS01580 (PUN50_01580) pilP 332835..333350 (+) 516 WP_005432702.1 pilus assembly protein PilP Machinery gene
  PUN50_RS01585 (PUN50_01585) pilQ 333385..335133 (+) 1749 WP_045369316.1 type IV pilus secretin PilQ Machinery gene
  PUN50_RS01590 (PUN50_01590) aroK 335322..335840 (+) 519 WP_010443904.1 shikimate kinase AroK -
  PUN50_RS01595 (PUN50_01595) aroB 335872..336972 (+) 1101 WP_045380637.1 3-dehydroquinate synthase -
  PUN50_RS01600 (PUN50_01600) - 337026..338519 (+) 1494 WP_274290765.1 SPOR domain-containing protein -
  PUN50_RS01605 (PUN50_01605) - 338589..339428 (+) 840 WP_038889578.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 582 a.a.        Molecular weight: 63787.73 Da        Isoelectric Point: 4.5130

>NTDB_id=788947 PUN50_RS01585 WP_045369316.1 333385..335133(+) (pilQ) [Vibrio campbellii strain 20220413_1]
MKMRHGLTLTPRKLTGLFSLWFFLLLAPFVAAEEVQSNSLKSIDFRTNKDKDAVIVVELASPAAIVDIKRVQEGLSIDLL
NTSVKDDQLYLLDVKDFSTVVESVEVFRDTSTTRLVAYIDEDYSHDYRLTGRYLEVKVGKLKANEKAPDKSILEKEGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKDELDLREKQQ
LEKQKLEKEIGELSSEIIKVNFAKASDIAEMINGEGNISMLSDRGSMTIDERTNSLLIRELPENIEVIREIIESLDIPVK
QVQIEARIVTINEGNMDELGVRWGFTSINGSHTVGGSIENNLATIGLYDGGGDGEGDDGGSSVGIDDFLNVNLAATSPNA
TSIAFQVAKLGSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVTFKKAVLSLKVTPQ
ITPDNRLVLDLSVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGALFR
RSYENVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1749 bp        

>NTDB_id=788947 PUN50_RS01585 WP_045369316.1 333385..335133(+) (pilQ) [Vibrio campbellii strain 20220413_1]
ATGAAAATGAGACACGGATTAACACTGACACCAAGGAAGCTAACAGGGCTCTTCTCTTTATGGTTCTTTCTACTTTTAGC
CCCTTTCGTCGCAGCAGAAGAAGTACAGTCTAACTCGCTTAAGAGCATCGACTTCCGTACGAACAAAGACAAAGACGCCG
TGATTGTGGTTGAGCTCGCATCACCCGCTGCGATTGTTGATATTAAACGCGTACAAGAAGGCTTGAGTATCGACTTACTC
AACACCTCCGTTAAAGATGACCAGCTTTACCTACTTGATGTGAAAGATTTCTCGACGGTTGTCGAGAGCGTTGAGGTATT
CCGCGATACTTCTACAACACGTCTGGTCGCTTATATCGATGAGGACTATTCTCACGACTACCGTCTTACTGGTCGTTATT
TAGAGGTAAAGGTTGGCAAGCTTAAAGCCAACGAAAAAGCGCCGGATAAAAGCATTTTGGAAAAAGAAGGCAAGCTAATT
TCGATTAACTTCCAAGACATTCCTGTGCGAAACGTGTTGCAACTTATCGCCGACTATAACCAATTTAACTTGGTCGTCTC
TGACTCGGTTGAGGGTAACTTGACCTTGCGTTTGGATGGTGTGCCTTGGCAGCAAGTACTCGACATTATCTTGCAAGTGA
AAGGGTTAGATAAGCGTGTTGATGGCAATGTCATTCTCGTTGCACCAAAAGATGAATTGGATCTGCGCGAGAAGCAGCAA
CTCGAAAAGCAAAAGCTAGAGAAAGAGATCGGTGAGCTTTCATCAGAGATCATCAAGGTGAATTTCGCTAAAGCGTCAGA
CATTGCTGAGATGATCAATGGGGAGGGCAACATCAGTATGCTTTCTGATCGAGGCAGCATGACCATTGATGAGCGTACCA
ACTCGTTATTGATTCGTGAGTTACCTGAGAATATTGAAGTGATTCGCGAGATCATAGAATCATTGGATATTCCTGTAAAA
CAGGTTCAGATTGAAGCGCGTATTGTGACTATTAATGAAGGTAACATGGACGAACTTGGCGTTCGTTGGGGCTTCACTTC
CATTAATGGCAGTCATACTGTGGGTGGTTCAATTGAGAATAACCTTGCGACAATCGGCCTGTACGATGGCGGCGGTGATG
GTGAAGGTGATGACGGTGGCAGCAGTGTGGGTATTGATGACTTTCTCAACGTTAACCTTGCCGCGACCAGTCCAAATGCG
ACCAGTATAGCCTTCCAAGTTGCGAAGTTAGGTTCGGATACCTTACTAGATTTGGAACTTTCGGCGCTGCAACAAGAATC
AAAAGCAGAAATTATTTCCAGTCCACGTTTGATCACCACGAATAAGAAGCCAGCTTACATTGAGCAGGGTACCGAGATCC
CTTACCTTGAATCTTCTTCCAGTGGTGCGACTTCTGTCACGTTCAAAAAAGCGGTATTGAGCTTGAAAGTGACGCCACAG
ATTACGCCTGACAACCGTTTGGTGTTGGATTTGAGCGTTACGCAAGACAGACCGGGCCAAGTGGTAAAAACAGGCACTGG
TGAGGCGGTGGCGATCGATACGCAACGTATTGGTACACAAGTTTTAGTCAATAATGGCGAGACAGTCGTGTTGGGCGGTA
TTTTTCAACACAGCATTACAAACTCCGTCGATAAGGTGCCTTTATTAGGTGATTTGCCGCTGTTAGGTGCGCTTTTCCGT
CGAAGTTACGAAAATGTCGGTAAAAGTGAACTACTGATTTTTGTTACACCTAAAGTTGTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(256-321)

Bacterial type II and III secretion system protein (Secretin)

(422-580)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

99.141

100

0.991
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

77.465

97.595

0.756
  pilQ Vibrio cholerae strain A1552

77.465

97.595

0.756
  pilQ Legionella pneumophila strain ERS1305867

41.742

94.674

0.395
  pilQ Legionella pneumophila strain Lp02

41.742

94.674

0.395
  pilQ Pseudomonas aeruginosa PAK

40.32

96.735

0.39
  pilQ Acinetobacter baumannii D1279779

37.271

100

0.385
  pilQ Acinetobacter baumannii strain A118

37.271

100

0.385
  comQ Acinetobacter baylyi ADP1

37.005

99.828

0.369