Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   PUN50_RS01570 Genome accession   NZ_CP117988
Coordinates   331684..332259 (+) Length   191 a.a.
NCBI ID   WP_005432710.1    Uniprot ID   -
Organism   Vibrio campbellii strain 20220413_1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 326684..337259
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUN50_RS01555 (PUN50_01555) oxyR 326952..327860 (+) 909 WP_005432705.1 DNA-binding transcriptional regulator OxyR -
  PUN50_RS01560 (PUN50_01560) - 327938..330505 (-) 2568 WP_038889585.1 penicillin-binding protein 1A -
  PUN50_RS01565 (PUN50_01565) pilM 330684..331700 (+) 1017 WP_274290764.1 type IV pilus biogenesis protein PilM Machinery gene
  PUN50_RS01570 (PUN50_01570) pilN 331684..332259 (+) 576 WP_005432710.1 PilN domain-containing protein Machinery gene
  PUN50_RS01575 (PUN50_01575) pilO 332252..332845 (+) 594 WP_005432718.1 type 4a pilus biogenesis protein PilO Machinery gene
  PUN50_RS01580 (PUN50_01580) pilP 332835..333350 (+) 516 WP_005432702.1 pilus assembly protein PilP Machinery gene
  PUN50_RS01585 (PUN50_01585) pilQ 333385..335133 (+) 1749 WP_045369316.1 type IV pilus secretin PilQ Machinery gene
  PUN50_RS01590 (PUN50_01590) aroK 335322..335840 (+) 519 WP_010443904.1 shikimate kinase AroK -
  PUN50_RS01595 (PUN50_01595) aroB 335872..336972 (+) 1101 WP_045380637.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 191 a.a.        Molecular weight: 22194.53 Da        Isoelectric Point: 9.4267

>NTDB_id=788944 PUN50_RS01570 WP_005432710.1 331684..332259(+) (pilN) [Vibrio campbellii strain 20220413_1]
MLYHINLLAWRENQREEHRRRFIGLILLGAIAALGVQWGIGKYYQYQQDQQQQRLDYLAHYISQLDKRIEAMKIAEQEHS
KILERLKTVEGLQNGRNKTTEFMNLMPSVIPEGVYVDKIKMNDYEIEISGISDSTSRLATMLDNMERSAKLLDVDMHSIV
HGKARFGKEFQTFKVSFMFKDASIKGGKQHG

Nucleotide


Download         Length: 576 bp        

>NTDB_id=788944 PUN50_RS01570 WP_005432710.1 331684..332259(+) (pilN) [Vibrio campbellii strain 20220413_1]
ATGCTGTATCACATTAACCTTCTTGCCTGGCGTGAAAACCAACGTGAAGAGCATCGTCGTCGTTTTATTGGCTTGATTTT
ACTCGGGGCGATCGCTGCTCTGGGCGTGCAATGGGGGATCGGTAAGTACTACCAATACCAACAAGATCAGCAGCAACAGC
GCTTGGATTATCTTGCTCACTATATCTCTCAACTCGATAAACGTATTGAAGCCATGAAGATAGCAGAGCAAGAGCACAGC
AAAATTCTAGAGCGCTTAAAGACCGTTGAGGGGCTGCAAAATGGGCGCAATAAAACCACTGAGTTTATGAACCTGATGCC
CTCAGTGATCCCTGAAGGCGTCTATGTCGATAAGATCAAAATGAACGACTACGAGATTGAGATCTCGGGTATTTCTGATA
GCACCTCTCGTCTTGCCACCATGCTCGATAATATGGAGCGTTCCGCCAAGCTGCTGGATGTTGATATGCACTCCATTGTT
CATGGTAAAGCGCGCTTTGGTAAAGAGTTCCAGACGTTCAAAGTCTCCTTCATGTTTAAAGACGCCTCTATAAAAGGAGG
TAAGCAGCATGGTTAA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio campbellii strain DS40M4

99.476

100

0.995
  pilN Vibrio cholerae strain A1552

60.452

92.67

0.56