Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   PUN50_RS01565 Genome accession   NZ_CP117988
Coordinates   330684..331700 (+) Length   338 a.a.
NCBI ID   WP_274290764.1    Uniprot ID   -
Organism   Vibrio campbellii strain 20220413_1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 325684..336700
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUN50_RS01550 (PUN50_01550) - 326049..326777 (-) 729 WP_005432732.1 redoxin family protein -
  PUN50_RS01555 (PUN50_01555) oxyR 326952..327860 (+) 909 WP_005432705.1 DNA-binding transcriptional regulator OxyR -
  PUN50_RS01560 (PUN50_01560) - 327938..330505 (-) 2568 WP_038889585.1 penicillin-binding protein 1A -
  PUN50_RS01565 (PUN50_01565) pilM 330684..331700 (+) 1017 WP_274290764.1 type IV pilus biogenesis protein PilM Machinery gene
  PUN50_RS01570 (PUN50_01570) pilN 331684..332259 (+) 576 WP_005432710.1 PilN domain-containing protein Machinery gene
  PUN50_RS01575 (PUN50_01575) pilO 332252..332845 (+) 594 WP_005432718.1 type 4a pilus biogenesis protein PilO Machinery gene
  PUN50_RS01580 (PUN50_01580) pilP 332835..333350 (+) 516 WP_005432702.1 pilus assembly protein PilP Machinery gene
  PUN50_RS01585 (PUN50_01585) pilQ 333385..335133 (+) 1749 WP_045369316.1 type IV pilus secretin PilQ Machinery gene
  PUN50_RS01590 (PUN50_01590) aroK 335322..335840 (+) 519 WP_010443904.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37874.32 Da        Isoelectric Point: 6.3141

>NTDB_id=788943 PUN50_RS01565 WP_274290764.1 330684..331700(+) (pilM) [Vibrio campbellii strain 20220413_1]
MDKLLVTGIDIGHNSLKAVVLKPNGNSYALLGYKEIILKEGIVAENNTINHQEIVKTLKEMKKELPFRARKVAISVPDNA
MISKKLQIEQNLEESEKEFAVVQAFSHQSPFPVEELSLDFIKVDTDEQMRGTDSYQVFATRKDVVETRVEALQKSGLKPV
LVDVHSQSLGHMWKLVAERFPEKNKYCLLDIGSLNSSFTIFTEQGELFHKEFPCGTRTTGSLFQQDPLADDAQVATEQFN
RQIVERVKRQMQLYTSINGSQDIKGVWLSGEGAATPMLAEELSSQLALECELLNPLGLFEMKVSKRKRRAADWQHFTAAA
GLAVRGIQWLGGDNAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=788943 PUN50_RS01565 WP_274290764.1 330684..331700(+) (pilM) [Vibrio campbellii strain 20220413_1]
ATGGATAAGCTATTAGTTACGGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTGGTACTTAAACCCAATGGCAACAG
TTACGCACTACTGGGTTACAAAGAAATAATACTTAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTTAAAGAGATGAAAAAAGAGCTACCTTTTCGGGCTCGAAAGGTCGCAATTTCAGTTCCAGATAATGCT
ATGATCAGTAAAAAGCTGCAGATAGAGCAGAACTTAGAAGAGAGTGAAAAGGAGTTTGCTGTTGTGCAAGCCTTCTCACA
TCAGTCTCCATTCCCCGTGGAAGAGCTGAGTCTTGACTTCATCAAGGTTGATACGGATGAGCAGATGCGAGGCACTGACA
GTTATCAGGTCTTCGCGACTCGTAAAGACGTGGTAGAAACCCGAGTTGAGGCATTGCAGAAATCAGGCTTGAAACCAGTA
CTGGTGGACGTGCACTCACAAAGTCTTGGTCATATGTGGAAATTGGTAGCGGAACGTTTTCCTGAAAAGAACAAGTATTG
TTTACTGGACATTGGTTCTTTGAACAGTTCTTTCACTATTTTCACTGAACAAGGTGAATTGTTTCATAAAGAATTTCCCT
GCGGTACTCGCACTACGGGCAGTTTGTTTCAACAAGATCCATTAGCAGACGATGCACAAGTGGCAACGGAGCAATTTAAT
CGACAAATCGTTGAGCGTGTTAAACGTCAGATGCAGCTCTATACCTCAATTAATGGAAGCCAAGATATCAAAGGCGTTTG
GCTTTCGGGAGAGGGCGCTGCAACACCTATGTTGGCCGAGGAGCTGTCTAGCCAGTTGGCACTAGAGTGTGAACTGCTTA
ATCCACTTGGTTTGTTTGAAATGAAGGTCTCTAAACGCAAACGTCGTGCCGCGGATTGGCAACACTTCACTGCTGCAGCC
GGACTGGCTGTACGTGGTATTCAGTGGCTAGGAGGTGACAATGCTGTATCACATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

97.633

100

0.976
  pilM Vibrio cholerae strain A1552

49.123

100

0.497