Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PUB83_RS00255 Genome accession   NZ_CP117918
Coordinates   45095..46204 (-) Length   369 a.a.
NCBI ID   WP_065475661.1    Uniprot ID   -
Organism   Aeromonas hydrophila subsp. hydrophila strain PSKL.DP     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 40095..51204
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUB83_RS00235 (PUB83_19925) brnQ 40552..41859 (-) 1308 WP_201904084.1 branched-chain amino acid transport system II carrier protein -
  PUB83_RS00240 (PUB83_19930) - 42051..42761 (-) 711 WP_274277641.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  PUB83_RS00245 (PUB83_19935) srmB 42890..44113 (+) 1224 WP_016351974.1 ATP-dependent RNA helicase SrmB -
  PUB83_RS00250 (PUB83_19940) yaaA 44290..45063 (-) 774 WP_274277642.1 peroxide stress protein YaaA -
  PUB83_RS00255 (PUB83_19945) pilU 45095..46204 (-) 1110 WP_065475661.1 type IVa pilus ATPase TapU Machinery gene
  PUB83_RS00260 (PUB83_19950) pilT 46233..47267 (-) 1035 WP_011707389.1 type IVa pilus ATPase TapT Machinery gene
  PUB83_RS00265 (PUB83_19955) - 47307..48008 (+) 702 WP_135318968.1 YggS family pyridoxal phosphate-dependent enzyme -
  PUB83_RS00270 (PUB83_19960) proC 48067..48891 (+) 825 WP_274277643.1 pyrroline-5-carboxylate reductase -
  PUB83_RS00275 (PUB83_19965) - 48932..49483 (+) 552 WP_016351969.1 YggT family protein -
  PUB83_RS00280 (PUB83_19970) yggU 49483..49782 (+) 300 WP_274277644.1 DUF167 family protein YggU -
  PUB83_RS00285 (PUB83_19975) - 49801..50220 (+) 420 WP_011707384.1 DUF4426 domain-containing protein -
  PUB83_RS00290 (PUB83_19980) - 50268..50579 (-) 312 WP_042064670.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41049.19 Da        Isoelectric Point: 6.5035

>NTDB_id=788311 PUB83_RS00255 WP_065475661.1 45095..46204(-) (pilU) [Aeromonas hydrophila subsp. hydrophila strain PSKL.DP]
MNLDALLTELVARKGSDLFITVGTPPTLKVNGHLQSLAEAPLDKARALALVKETLSQEHFERYLHTKEANYAIHREELGR
FRVSAFWQQELPGMVLRRIETRIPTFDELALPPILQEIALAKRGLVLFVGATGAGKSTTQAAMIGYRNQHVDGHILTVED
PVEFVHQHGRSLITQREVGIDTASFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSLDGRRRCAAFEVLLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFALFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=788311 PUB83_RS00255 WP_065475661.1 45095..46204(-) (pilU) [Aeromonas hydrophila subsp. hydrophila strain PSKL.DP]
ATGAATCTGGATGCCCTGCTGACCGAGCTGGTCGCCCGCAAAGGCTCGGATCTGTTCATCACGGTCGGCACACCGCCGAC
CCTCAAGGTCAACGGCCACCTGCAGTCACTGGCAGAAGCCCCCCTCGACAAGGCCAGGGCGCTGGCGCTGGTCAAGGAGA
CCCTGAGCCAGGAGCACTTTGAGCGCTACCTGCACACCAAGGAGGCCAACTACGCCATCCACCGCGAGGAGCTTGGCCGC
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCGGGCATGGTGCTGCGCCGCATCGAGACCCGCATCCCCACCTTCGA
CGAGCTGGCGCTGCCCCCCATCCTGCAGGAAATAGCCCTGGCCAAGCGCGGGCTGGTGCTGTTCGTCGGAGCTACCGGCG
CCGGCAAATCCACCACCCAGGCGGCCATGATCGGCTATCGCAACCAGCATGTGGACGGCCACATCCTGACGGTGGAAGAT
CCGGTGGAGTTCGTGCATCAGCACGGCCGCAGCCTGATCACCCAGCGCGAGGTGGGCATAGACACAGCGTCATTCGACGT
GGCGCTGAAGAGTTCACTGCGCCAGGCTCCGGACGTGATCCTGATCGGCGAAATTCGCAGCCAGGAGACCATGGAGTTTG
CCCTGCAGTTTGCCGAGACCGGCCATCTCTGCCTCGCAACCCTGCACGCCAACAATGCCAATCAGGCGCTGGACCGCATC
CTGCACCTGGTCCCTCAGGAGAAACATCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGTGCCATCGTGGCCCAGCA
ACTGCTGCCCAGCCTGGACGGGCGCCGTCGCTGTGCGGCCTTCGAGGTGCTGCTCAACACCCCGCTCATCACCGATATCA
TCCGCAAGGGGGAGATGCACCGGCTCAAGGAGGTGATGACCAAGTCCACCGAGCTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCGCCCTGTTCTGTGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTGTCGGGGCGCGAGCAGCTGGGTGCAGGCACTCTCGACAACGTCACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.598

99.729

0.604
  pilU Pseudomonas stutzeri DSM 10701

59.544

95.122

0.566
  pilU Acinetobacter baylyi ADP1

57.224

95.664

0.547
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.296

89.702

0.379
  pilT Legionella pneumophila strain Lp02

40.525

92.954

0.377
  pilT Legionella pneumophila strain ERS1305867

40.525

92.954

0.377
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.12

90.515

0.363
  pilT Vibrio cholerae strain A1552

40.12

90.515

0.363
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363