Detailed information NTG0785719

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PSH69_RS12180
• Genome accession: NZ_CP117461
• Coordinates: 2671907..2673016 (+)
• Length: 369 a.a.
• NCBI ID: WP_305412200.1
• Uniprot ID: -
• Organism: Pseudomonas sp. FP1740
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2666907..2678016

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PSH69_RS12165 (PSH69_12160)	-	2667906..2668841 (+)	936	WP_305412198.1	sugar kinase	-
PSH69_RS12170 (PSH69_12165)	-	2668907..2669182 (-)	276	WP_007933743.1	peptidylprolyl isomerase	-
PSH69_RS12175 (PSH69_12170)	-	2669249..2671786 (-)	2538	WP_305412199.1	PAS domain S-box protein	-
PSH69_RS12180 (PSH69_12175)	pilU	2671907..2673016 (+)	1110	WP_305412200.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PSH69_RS12185 (PSH69_12180)	-	2673073..2675028 (-)	1956	WP_305412202.1	acetoacetate--CoA ligase	-
PSH69_RS12190 (PSH69_12185)	hbdH	2675223..2675996 (-)	774	WP_018927665.1	3-hydroxybutyrate dehydrogenase	-
PSH69_RS12195 (PSH69_12190)	-	2676004..2677395 (-)	1392	WP_305412206.1	GntP family permease	-

Sequence

Protein

• Length: 369 a.a.
• Molecular weight: 40750.63 Da
• Isoelectric Point: 6.6989

>NTDB_id=785719 PSH69_RS12180 WP_305412200.1 2671907..2673016(+) (pilU) [Pseudomonas sp. FP1740]
MEIDALLQILASRHGSDLYLSTGAPPSAKFDGVLKALTDQPFKPGEVAAIAASIMDAEQRLEFDRDLEMNLALSLAGVGR
FRLNIFKQRNDVSIVARNIKLDIPRFEDLKLPPVLLETVMLKQGLILFVGSTGSGKSTSLAALVDYRNRHSSGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVISTLHAHNANQALDRV
INFFPEERRGQLLNDLGNNLKAFVSQRLVRTRDGQRRAAVEVMLGTPTIGDLIRRNEFGELKGIMEKSSEVGMQTFDGAL
FALFAEGAIDEAEALKHADSVNNLRLRLKLHAEATPGPHSPPGEWGLMD

Nucleotide

• Length: 1110 bp

>NTDB_id=785719 PSH69_RS12180 WP_305412200.1 2671907..2673016(+) (pilU) [Pseudomonas sp. FP1740]
ATGGAAATCGATGCACTGCTGCAGATTCTGGCCAGCCGCCATGGCTCCGATCTTTACCTGTCCACGGGCGCGCCACCCAG
CGCGAAATTTGACGGTGTGCTCAAAGCCCTGACCGATCAACCGTTCAAGCCGGGAGAAGTGGCGGCCATTGCCGCGTCCA
TCATGGACGCCGAACAACGCCTGGAGTTCGATCGGGACCTGGAAATGAACCTGGCGCTTTCCCTGGCCGGGGTCGGGCGT
TTCCGGCTCAATATCTTCAAGCAGCGCAATGATGTGTCCATCGTGGCGCGCAACATCAAACTCGACATTCCACGCTTCGA
AGACCTCAAGCTGCCGCCGGTGCTGCTCGAAACGGTGATGCTCAAACAGGGGTTGATACTGTTCGTCGGGTCGACCGGCT
CCGGCAAGTCGACCTCGCTGGCGGCATTGGTCGACTACCGCAACCGCCATAGTAGCGGCCATATCATCACCATCGAAGAC
CCCGTCGAGTTTATCCATCGGCACAAAAAGTCGATCGTCAATCAGCGCGAGGTCGGCGTCGATACCCGCAGTTTCCACGC
CGCGCTGAAAAACACGTTGCGCCAGGCGCCGGACGTGGTGTTGATCGGCGAAATCCGTGACCGCGAGACTATGGAGCATG
CGCTGGCGTTTGCCGATACCGGTCATCTGGTAATTTCCACGTTGCATGCGCACAACGCCAATCAGGCGCTGGACCGCGTG
ATTAACTTCTTTCCCGAAGAGCGCCGGGGGCAGTTGCTCAACGACCTGGGCAACAACCTCAAAGCGTTTGTGTCCCAGCG
TCTGGTGCGTACCCGCGACGGGCAGCGCCGGGCGGCGGTGGAGGTGATGCTGGGCACACCAACCATCGGCGACCTGATCC
GGCGCAATGAGTTTGGCGAACTCAAGGGGATCATGGAGAAGTCTTCAGAGGTGGGAATGCAGACGTTTGACGGGGCGCTG
TTCGCGCTGTTTGCCGAGGGCGCAATCGATGAGGCAGAGGCGTTGAAACATGCTGATTCGGTGAACAACTTGCGCTTGCG
CCTGAAATTGCACGCCGAGGCCACGCCCGGTCCCCATTCACCGCCGGGGGAATGGGGGTTGATGGACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	54.891	99.729	0.547
pilU	Acinetobacter baylyi ADP1	52.809	96.477	0.509
pilU	Vibrio cholerae strain A1552	51.143	94.851	0.485
pilT	Pseudomonas stutzeri DSM 10701	40.698	93.225	0.379
pilT	Pseudomonas aeruginosa PAK	40.407	93.225	0.377
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	41.441	90.244	0.374
pilT	Vibrio cholerae strain A1552	41.441	90.244	0.374
pilT	Legionella pneumophila strain Lp02	40.419	90.515	0.366
pilT	Legionella pneumophila strain ERS1305867	40.419	90.515	0.366
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.233	91.87	0.36