Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PSH64_RS25750 Genome accession   NZ_CP117460
Coordinates   5692222..5693439 (+) Length   405 a.a.
NCBI ID   WP_105343830.1    Uniprot ID   A0A2S8HKE6
Organism   Pseudomonas sp. FP1742     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5687222..5698439
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH64_RS25730 (PSH64_25725) - 5688729..5689025 (+) 297 WP_305481211.1 DUF2845 domain-containing protein -
  PSH64_RS25735 (PSH64_25730) - 5689200..5689556 (-) 357 WP_305479120.1 BON domain-containing protein -
  PSH64_RS25740 (PSH64_25735) - 5689879..5690292 (-) 414 WP_305479121.1 pilin -
  PSH64_RS25745 (PSH64_25740) pilB 5690519..5692219 (+) 1701 WP_305479122.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSH64_RS25750 (PSH64_25745) pilC 5692222..5693439 (+) 1218 WP_105343830.1 type II secretion system F family protein Machinery gene
  PSH64_RS25755 (PSH64_25750) pilD 5693441..5694313 (+) 873 WP_105343828.1 A24 family peptidase Machinery gene
  PSH64_RS25760 (PSH64_25755) coaE 5694310..5694933 (+) 624 WP_305479123.1 dephospho-CoA kinase -
  PSH64_RS25765 (PSH64_25760) yacG 5694930..5695130 (+) 201 WP_030130376.1 DNA gyrase inhibitor YacG -
  PSH64_RS25770 (PSH64_25765) - 5695140..5695355 (-) 216 WP_105343824.1 hypothetical protein -
  PSH64_RS25775 (PSH64_25770) - 5695419..5696108 (-) 690 WP_105343821.1 energy-coupling factor ABC transporter permease -
  PSH64_RS25780 (PSH64_25775) - 5696352..5697455 (+) 1104 WP_105343819.1 AI-2E family transporter -
  PSH64_RS25785 (PSH64_25780) - 5697478..5697909 (+) 432 WP_105343817.1 potassium channel family protein -
  PSH64_RS25790 (PSH64_25785) - 5697936..5698403 (-) 468 WP_105343815.1 FAD/FMN-containing dehydrogenase -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44130.03 Da        Isoelectric Point: 9.8359

>NTDB_id=785702 PSH64_RS25750 WP_105343830.1 5692222..5693439(+) (pilC) [Pseudomonas sp. FP1742]
MAVKAAKISVYAWEGTDRKGAKMTGELTGQNPALIKAQLRKQGINPGKVRKKSASIFSFGKRIKALDIALFTRQMATMMK
AGVPLLQSFEIIGEGFDNPNMRKLVDEVKQEVAAGNSFAAALRKKPEYFDELYCNLVDAGEQAGALDTLLERVATYKEKS
ESLKAKIKKAMTYPLAVVFVAIIVTGILLVKVVPQFQSVFSGFGAELPAFTVMVIGLSEFMQDWWWMMLGVLGALIFGLR
HAFKNSPGFRDRTDAWLLKLPLVGALMYKSAVARYARTLSTTFAAGVPLVEALESVAGATGNIVFKRAVLRIKQDVSTGM
QLNFSMRTSGIFPTMAIQMTAIGEESGALDDMLDKVAGFYEAEVDNMVDNLTSLMEPFIMVVLGVIVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=785702 PSH64_RS25750 WP_105343830.1 5692222..5693439(+) (pilC) [Pseudomonas sp. FP1742]
ATGGCGGTCAAAGCAGCGAAAATCAGCGTGTATGCCTGGGAAGGCACGGATCGCAAAGGCGCGAAGATGACCGGCGAGTT
GACCGGGCAAAATCCCGCGCTGATCAAGGCTCAATTGCGCAAGCAGGGGATCAACCCGGGCAAGGTGCGGAAAAAGTCCG
CGTCCATTTTCAGTTTCGGCAAACGCATCAAGGCCCTGGACATTGCCCTGTTCACCCGGCAGATGGCGACCATGATGAAA
GCTGGCGTACCGCTGCTGCAATCGTTCGAAATCATCGGCGAAGGCTTCGATAACCCGAACATGCGCAAGCTGGTGGACGA
GGTGAAACAGGAAGTTGCGGCCGGCAACAGCTTCGCCGCGGCCCTGCGCAAAAAGCCCGAGTATTTCGATGAGCTGTACT
GCAATCTGGTGGACGCCGGCGAGCAGGCCGGTGCCCTGGACACCTTGCTGGAACGGGTGGCGACCTACAAGGAAAAGAGC
GAAAGCCTCAAGGCCAAGATCAAGAAAGCCATGACCTACCCGCTGGCCGTGGTGTTCGTCGCGATCATCGTGACCGGGAT
TCTGCTGGTCAAGGTTGTACCGCAGTTCCAGTCGGTATTCTCGGGGTTCGGTGCCGAGCTGCCGGCGTTCACCGTGATGG
TCATCGGCTTGTCGGAATTCATGCAGGATTGGTGGTGGATGATGCTTGGCGTACTGGGAGCGCTGATCTTCGGCCTGCGC
CACGCCTTCAAGAACTCTCCAGGTTTTCGCGACCGGACGGACGCCTGGCTGCTGAAGCTGCCGTTGGTTGGCGCGCTCAT
GTACAAGTCCGCCGTGGCCCGTTACGCCCGCACCCTGTCGACGACGTTTGCCGCCGGGGTGCCACTGGTCGAAGCCCTGG
AGTCAGTGGCAGGCGCGACCGGCAATATCGTGTTCAAGCGAGCGGTGCTGCGCATCAAGCAGGACGTTTCCACCGGCATG
CAGCTTAACTTTTCCATGCGCACCTCCGGCATTTTTCCGACCATGGCGATCCAGATGACCGCCATTGGCGAAGAGTCCGG
CGCCCTGGACGACATGCTCGACAAGGTCGCGGGTTTCTACGAGGCCGAGGTGGACAACATGGTCGATAACCTGACCAGTC
TGATGGAGCCGTTCATCATGGTGGTGCTGGGGGTCATCGTCGGCGGCCTGGTGGTTGCAATGTACCTGCCCATCTTCCAA
CTCGGCTCAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S8HKE6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

73.892

100

0.741
  pilC Acinetobacter baylyi ADP1

60.784

100

0.612
  pilC Acinetobacter baumannii D1279779

60.049

100

0.605
  pilC Legionella pneumophila strain ERS1305867

53.03

97.778

0.519
  pilG Neisseria gonorrhoeae MS11

42.928

99.506

0.427
  pilG Neisseria meningitidis 44/76-A

42.432

99.506

0.422
  pilC Vibrio cholerae strain A1552

42.929

97.778

0.42
  pilC Vibrio campbellii strain DS40M4

41.523

100

0.417
  pilC Thermus thermophilus HB27

37.905

99.012

0.375