Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PSH64_RS25745 Genome accession   NZ_CP117460
Coordinates   5690519..5692219 (+) Length   566 a.a.
NCBI ID   WP_305479122.1    Uniprot ID   -
Organism   Pseudomonas sp. FP1742     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5685519..5697219
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH64_RS25725 (PSH64_25720) - 5685736..5688729 (+) 2994 WP_305479119.1 DUF748 domain-containing protein -
  PSH64_RS25730 (PSH64_25725) - 5688729..5689025 (+) 297 WP_305481211.1 DUF2845 domain-containing protein -
  PSH64_RS25735 (PSH64_25730) - 5689200..5689556 (-) 357 WP_305479120.1 BON domain-containing protein -
  PSH64_RS25740 (PSH64_25735) - 5689879..5690292 (-) 414 WP_305479121.1 pilin -
  PSH64_RS25745 (PSH64_25740) pilB 5690519..5692219 (+) 1701 WP_305479122.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSH64_RS25750 (PSH64_25745) pilC 5692222..5693439 (+) 1218 WP_105343830.1 type II secretion system F family protein Machinery gene
  PSH64_RS25755 (PSH64_25750) pilD 5693441..5694313 (+) 873 WP_105343828.1 A24 family peptidase Machinery gene
  PSH64_RS25760 (PSH64_25755) coaE 5694310..5694933 (+) 624 WP_305479123.1 dephospho-CoA kinase -
  PSH64_RS25765 (PSH64_25760) yacG 5694930..5695130 (+) 201 WP_030130376.1 DNA gyrase inhibitor YacG -
  PSH64_RS25770 (PSH64_25765) - 5695140..5695355 (-) 216 WP_105343824.1 hypothetical protein -
  PSH64_RS25775 (PSH64_25770) - 5695419..5696108 (-) 690 WP_105343821.1 energy-coupling factor ABC transporter permease -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62941.94 Da        Isoelectric Point: 5.6963

>NTDB_id=785701 PSH64_RS25745 WP_305479122.1 5690519..5692219(+) (pilB) [Pseudomonas sp. FP1742]
MNDIALSGLAKQLVLAELITDQSAQQAYQQAQRNRVSLVSYLVQNKLLKSWQVAEVASEHFGMALLDLSCLDKDTQPRGL
VSEKLVRQHHALPLWRRGNKLFVGISDPTNHQAINDIQFSTGLSTEAILVEEDKLGDAIEKFFDSHTTGLEEMADVDLDG
LDIESVDDQKQDSIPGQDTDDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKTYRVRVRTDGMLREVAKPPTQLANRIASRL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPEQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINIEGINQVNVNPRQGMDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVLSTLHTNSAAQTLTRLHNMGIQGFNIATSISLIIAQRLARKLCSHCKKPIEIPRETLLKEGFPE
ERIGSFTIYEPVGCDQCNNGYKGRVGIYEVVKNTPDLQRLIMAEGNSLEIDLQMRKDGFNDLRTSGLLKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=785701 PSH64_RS25745 WP_305479122.1 5690519..5692219(+) (pilB) [Pseudomonas sp. FP1742]
ATGAATGACATTGCCCTTAGCGGTCTGGCCAAGCAATTGGTGCTCGCCGAACTGATCACCGATCAAAGTGCGCAACAGGC
GTATCAGCAAGCCCAACGCAATCGAGTCTCACTGGTCAGCTATCTGGTGCAGAACAAACTGCTGAAAAGCTGGCAGGTCG
CCGAGGTGGCCTCGGAGCATTTCGGCATGGCCCTGCTGGACCTCAGTTGCCTGGACAAAGACACCCAGCCCCGCGGACTG
GTCAGCGAGAAGCTGGTTCGCCAGCACCACGCCCTGCCCCTGTGGCGGCGAGGCAACAAGCTGTTCGTGGGAATTTCCGA
CCCGACCAATCATCAGGCCATCAATGACATCCAGTTCAGCACCGGGTTGAGCACCGAAGCCATCCTGGTGGAAGAAGACA
AACTCGGCGACGCGATCGAAAAATTCTTCGACAGTCATACCACTGGTCTGGAGGAGATGGCCGATGTCGACCTCGACGGG
CTGGACATCGAATCGGTCGATGACCAGAAACAAGATTCCATCCCCGGGCAGGATACCGATGACGCGCCCGTGGTGCGCTT
CGTCCACAAAATGTTGCTCGATGCGATCAAAAGCGGTTCTTCCGACCTGCACTTCGAGCCCTACGAAAAAACCTACCGCG
TGCGGGTGCGCACCGACGGCATGTTGCGGGAGGTCGCCAAGCCACCGACACAACTAGCCAACCGCATTGCTTCCCGGTTG
AAAGTCATGGCCAGCCTCGACATCTCCGAGCGGCGCAAACCCCAGGACGGGCGGATCAAGATGCGCCTGTCCAAAAGCAA
GTCCATCGACTTCCGGGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGGTGGTGATCCGGATCCTCGACCCGTCCAGTG
CGCAAATGGGCATCGACGCCCTCGGCTACGAGCCCGAGCAGAAAGACCTGTACATGGCCGCGCTCAAGCAGCCACAGGGG
ATGATCCTGGTGACCGGCCCCACCGGCTCGGGCAAGACCGTGTCGCTGTATACCGGGCTGAACATCCTCAACACCGTCGA
CATCAATATCTCTACCGCCGAGGATCCGGTAGAAATCAACATCGAAGGCATCAACCAGGTCAACGTCAACCCCAGGCAAG
GAATGGATTTCGCCCAGGCGTTGCGCTCGTTTCTGCGCCAGGACCCGGACGTGATCATGGTCGGTGAAATCCGTGACCTC
GAAACTGCCGAAATCGCCATCAAGGCCGCCCAGACCGGGCATATGGTGCTTTCCACCCTGCACACCAACAGCGCCGCGCA
AACCCTGACCCGCCTGCACAACATGGGGATTCAGGGGTTCAACATCGCGACCTCGATCAGCCTGATCATCGCCCAGCGCC
TGGCACGCAAACTGTGCAGCCATTGCAAGAAGCCCATCGAGATTCCCCGCGAGACGCTGCTCAAGGAAGGCTTCCCCGAG
GAACGCATCGGCTCGTTCACGATCTATGAACCGGTCGGTTGCGATCAGTGCAACAACGGTTACAAAGGGCGAGTCGGGAT
TTATGAAGTGGTGAAGAACACCCCGGACCTGCAACGGCTGATCATGGCCGAAGGCAATTCACTGGAAATCGACCTCCAGA
TGCGTAAGGACGGCTTCAACGACCTGCGCACGTCGGGGCTGCTCAAGGCCATGCAAGGCATCACCAGCCTTGAAGAAATC
AATCGGGTCACGAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.904

99.47

0.576
  pilB Acinetobacter baumannii D1279779

57.624

99.647

0.574
  pilB Legionella pneumophila strain ERS1305867

54.947

100

0.549
  pilB Vibrio cholerae strain A1552

50.619

99.823

0.505
  pilB Vibrio parahaemolyticus RIMD 2210633

49.46

98.233

0.486
  pilF Neisseria gonorrhoeae MS11

48.845

99.47

0.486
  pilB Vibrio campbellii strain DS40M4

47.895

100

0.482
  pilF Thermus thermophilus HB27

36.926

100

0.369
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.826

93.286

0.362