Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   PSH64_RS25755 Genome accession   NZ_CP117460
Coordinates   5693441..5694313 (+) Length   290 a.a.
NCBI ID   WP_105343828.1    Uniprot ID   A0A2S8HLB8
Organism   Pseudomonas sp. FP1742     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5688441..5699313
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH64_RS25730 (PSH64_25725) - 5688729..5689025 (+) 297 WP_305481211.1 DUF2845 domain-containing protein -
  PSH64_RS25735 (PSH64_25730) - 5689200..5689556 (-) 357 WP_305479120.1 BON domain-containing protein -
  PSH64_RS25740 (PSH64_25735) - 5689879..5690292 (-) 414 WP_305479121.1 pilin -
  PSH64_RS25745 (PSH64_25740) pilB 5690519..5692219 (+) 1701 WP_305479122.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSH64_RS25750 (PSH64_25745) pilC 5692222..5693439 (+) 1218 WP_105343830.1 type II secretion system F family protein Machinery gene
  PSH64_RS25755 (PSH64_25750) pilD 5693441..5694313 (+) 873 WP_105343828.1 A24 family peptidase Machinery gene
  PSH64_RS25760 (PSH64_25755) coaE 5694310..5694933 (+) 624 WP_305479123.1 dephospho-CoA kinase -
  PSH64_RS25765 (PSH64_25760) yacG 5694930..5695130 (+) 201 WP_030130376.1 DNA gyrase inhibitor YacG -
  PSH64_RS25770 (PSH64_25765) - 5695140..5695355 (-) 216 WP_105343824.1 hypothetical protein -
  PSH64_RS25775 (PSH64_25770) - 5695419..5696108 (-) 690 WP_105343821.1 energy-coupling factor ABC transporter permease -
  PSH64_RS25780 (PSH64_25775) - 5696352..5697455 (+) 1104 WP_105343819.1 AI-2E family transporter -
  PSH64_RS25785 (PSH64_25780) - 5697478..5697909 (+) 432 WP_105343817.1 potassium channel family protein -
  PSH64_RS25790 (PSH64_25785) - 5697936..5698403 (-) 468 WP_105343815.1 FAD/FMN-containing dehydrogenase -
  PSH64_RS25795 (PSH64_25790) - 5698532..5699158 (+) 627 WP_007938395.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 290 a.a.        Molecular weight: 32218.45 Da        Isoelectric Point: 7.4000

>NTDB_id=785703 PSH64_RS25755 WP_105343828.1 5693441..5694313(+) (pilD) [Pseudomonas sp. FP1742]
MSLNEFLSLNPLAFVIAAGLIGLLIGSFLNVVVWRLPKMLEREWRLQAHDVLDLPAEAPGPTYNLILPHSECPHCGHQIR
VWENIPILSYLFLRGRCSHCAAPISKRYPLTELACSLLSAFVAWHFGFGWQACMVLVLSWGLLAMSLIDAEHQLLPDVLV
MPLMWLGLILNSFGLFVSLHDAFWGAVAGYMALWTVFWLFKLVTGKDGIGYGDFKLLAMLGAWGGWQILPMTILLSSLVG
AILGVILLRLRDAKTSTPLPFGPYLAIAGWIALLWGGQITGFYWQFVGLK

Nucleotide


Download         Length: 873 bp        

>NTDB_id=785703 PSH64_RS25755 WP_105343828.1 5693441..5694313(+) (pilD) [Pseudomonas sp. FP1742]
ATGTCCTTGAACGAATTCCTGAGCCTCAACCCGCTGGCCTTCGTGATCGCCGCAGGGCTGATCGGCCTGCTGATCGGCAG
TTTTCTCAATGTGGTGGTCTGGCGCCTGCCGAAGATGCTTGAGCGTGAATGGCGCCTGCAGGCCCATGACGTGCTGGACT
TGCCAGCCGAAGCCCCCGGCCCGACCTACAACCTGATACTGCCCCACTCCGAGTGTCCTCACTGCGGCCATCAGATTCGG
GTCTGGGAAAACATACCGATACTCAGTTATCTGTTTTTACGCGGGCGCTGCTCACACTGCGCGGCGCCGATCAGTAAACG
TTACCCGCTGACCGAACTGGCTTGCAGCCTGCTGTCGGCGTTCGTCGCCTGGCATTTCGGTTTCGGCTGGCAGGCCTGCA
TGGTGCTGGTTCTGAGTTGGGGCCTGCTGGCGATGAGCCTGATCGATGCCGAGCATCAACTGTTGCCGGACGTACTGGTG
ATGCCGTTGATGTGGCTGGGGTTGATCCTCAACAGCTTCGGGCTCTTCGTATCACTGCATGATGCGTTCTGGGGCGCAGT
GGCCGGCTACATGGCGCTGTGGACGGTGTTCTGGCTGTTCAAGCTGGTCACCGGCAAGGACGGCATCGGTTACGGTGACT
TCAAGCTCCTGGCGATGCTCGGAGCCTGGGGCGGCTGGCAGATCCTGCCCATGACCATTCTGCTGTCATCGCTGGTAGGG
GCCATTCTCGGGGTGATTTTGCTGCGCCTGCGGGATGCGAAAACCTCGACGCCGCTACCCTTTGGACCCTATCTGGCCAT
TGCCGGCTGGATTGCCTTGCTCTGGGGTGGTCAAATAACCGGCTTCTATTGGCAGTTTGTCGGTTTGAAATGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-126)

Type IV leader peptidase family (Peptidase_A24)

(136-245)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S8HLB8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

53.546

97.241

0.521
  pilD Vibrio campbellii strain DS40M4

52.128

97.241

0.507
  pilD Acinetobacter baumannii D1279779

50

95.172

0.476
  pilD Acinetobacter nosocomialis M2

49.458

95.517

0.472
  pilD Neisseria gonorrhoeae MS11

47.407

93.103

0.441