Detailed information NTG0785696

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PSH64_RS17125
• Genome accession: NZ_CP117460
• Coordinates: 3874573..3875682 (-)
• Length: 369 a.a.
• NCBI ID: WP_305477909.1
• Uniprot ID: -
• Organism: Pseudomonas sp. FP1742
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3869573..3880682

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PSH64_RS17110 (PSH64_17105)	-	3870099..3871490 (+)	1392	WP_105341146.1	GntP family permease	-
PSH64_RS17115 (PSH64_17110)	hbdH	3871498..3872271 (+)	774	WP_105341149.1	3-hydroxybutyrate dehydrogenase	-
PSH64_RS17120 (PSH64_17115)	-	3872491..3874446 (+)	1956	WP_305477908.1	acetoacetate--CoA ligase	-
PSH64_RS17125 (PSH64_17120)	pilU	3874573..3875682 (-)	1110	WP_305477909.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PSH64_RS17130 (PSH64_17125)	-	3875801..3878338 (+)	2538	WP_305477910.1	PAS domain S-box protein	-
PSH64_RS17135 (PSH64_17130)	-	3878406..3878681 (+)	276	WP_007933743.1	peptidylprolyl isomerase	-
PSH64_RS17140 (PSH64_17135)	-	3878825..3879757 (-)	933	WP_305477911.1	sugar kinase	-

Sequence

Protein

• Length: 369 a.a.
• Molecular weight: 40763.65 Da
• Isoelectric Point: 6.5098

>NTDB_id=785696 PSH64_RS17125 WP_305477909.1 3874573..3875682(-) (pilU) [Pseudomonas sp. FP1742]
MEIDALLQILASRNGSDLYLSTGAPPSAKFDGVLKALAEQPFKPGEVAAIAASIMDAEQRLEFDRDLEMNLALSLAGVGR
FRLNIFKQRNDVSIVARNIKLDIPRFEDLKLPPVLLETVMLKQGLILFVGSTGSGKSTSLAALIDYRNRHSSGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVISTLHAHNANQALDRI
INFFPEERRAQLLNDLGNNLKAFVSQRLVRTRDGQRRAAVEVMLGTPTIGDLIRRNEFGELKSIMEKSSEAGMQTFDGAL
FELLAEGAIDEAEALKHADSVNNLRLRLKLHAEATPGPHSLPGEWGLVD

Nucleotide

• Length: 1110 bp

>NTDB_id=785696 PSH64_RS17125 WP_305477909.1 3874573..3875682(-) (pilU) [Pseudomonas sp. FP1742]
ATGGAAATCGATGCACTGTTGCAGATTCTGGCCAGCCGCAATGGCTCCGATCTTTACCTGTCCACGGGCGCGCCACCCAG
CGCGAAATTTGACGGTGTGCTCAAAGCCCTGGCCGAGCAACCATTCAAACCGGGAGAAGTGGCGGCCATTGCCGCGTCCA
TCATGGACGCCGAACAACGCCTGGAGTTCGATCGGGACCTGGAAATGAACCTGGCGCTTTCCCTGGCCGGGGTCGGGCGC
TTTCGACTCAATATCTTCAAGCAGCGCAACGATGTGTCCATCGTGGCGCGCAACATCAAACTCGACATCCCGCGCTTCGA
AGACCTCAAGCTGCCGCCGGTGCTGCTCGAAACGGTGATGCTCAAACAGGGGTTGATACTGTTCGTCGGGTCGACCGGCT
CCGGCAAGTCGACCTCGCTGGCGGCATTGATCGACTACCGCAACCGCCATAGTAGCGGCCATATCATCACCATCGAAGAC
CCCGTCGAGTTTATCCATCGGCACAAAAAGTCGATCGTCAATCAGCGCGAGGTCGGTGTCGATACCCGCAGTTTTCATGC
CGCGTTGAAAAACACCCTGCGCCAGGCACCGGACGTGGTGTTGATCGGTGAAATCCGCGACCGCGAAACCATGGAGCATG
CGCTGGCGTTTGCCGATACCGGTCATTTGGTGATTTCCACGTTACATGCGCACAACGCCAATCAGGCGCTGGACCGGATT
ATCAACTTCTTTCCCGAGGAACGCAGGGCGCAATTGCTCAATGACCTGGGCAATAACCTCAAGGCGTTTGTTTCCCAGCG
TTTGGTGCGTACCCGCGATGGGCAGCGCCGGGCGGCGGTGGAGGTGATGCTGGGAACGCCGACCATTGGTGACTTGATTC
GCCGCAATGAGTTTGGCGAACTCAAGAGCATCATGGAGAAGTCTTCAGAGGCGGGAATGCAGACGTTTGATGGTGCGCTG
TTCGAATTGCTTGCCGAGGGCGCCATTGATGAGGCAGAGGCGTTGAAACATGCCGATTCGGTGAACAACTTGCGCTTGCG
CCTGAAACTGCACGCCGAGGCTACGCCCGGTCCCCATTCACTGCCGGGGGAATGGGGATTAGTGGACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	55.014	100	0.55
pilU	Acinetobacter baylyi ADP1	53.371	96.477	0.515
pilU	Vibrio cholerae strain A1552	51.429	94.851	0.488
pilT	Pseudomonas stutzeri DSM 10701	40.988	93.225	0.382
pilT	Pseudomonas aeruginosa PAK	40.698	93.225	0.379
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	42.042	90.244	0.379
pilT	Vibrio cholerae strain A1552	42.042	90.244	0.379
pilT	Legionella pneumophila strain Lp02	41.617	90.515	0.377
pilT	Legionella pneumophila strain ERS1305867	41.617	90.515	0.377
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.823	91.87	0.366