Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PSH71_RS25770 Genome accession   NZ_CP117457
Coordinates   5787147..5788364 (+) Length   405 a.a.
NCBI ID   WP_003205670.1    Uniprot ID   A0AAD1GU29
Organism   Pseudomonas brassicacearum strain FP1843     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5782147..5793364
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH71_RS25750 (PSH71_25745) - 5783543..5783839 (+) 297 WP_013694228.1 DUF2845 domain-containing protein -
  PSH71_RS25755 (PSH71_25750) - 5784168..5784521 (-) 354 WP_003205664.1 BON domain-containing protein -
  PSH71_RS25760 (PSH71_25755) pilA 5784794..5785219 (-) 426 WP_013694230.1 pilin Machinery gene
  PSH71_RS25765 (PSH71_25760) pilB 5785444..5787144 (+) 1701 WP_003205669.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSH71_RS25770 (PSH71_25765) pilC 5787147..5788364 (+) 1218 WP_003205670.1 type II secretion system F family protein Machinery gene
  PSH71_RS25775 (PSH71_25770) pilD 5788366..5789235 (+) 870 WP_305441614.1 A24 family peptidase Machinery gene
  PSH71_RS25780 (PSH71_25775) coaE 5789235..5789858 (+) 624 WP_003205673.1 dephospho-CoA kinase -
  PSH71_RS25785 (PSH71_25780) yacG 5789855..5790061 (+) 207 WP_003205675.1 DNA gyrase inhibitor YacG -
  PSH71_RS25790 (PSH71_25785) - 5790072..5790287 (-) 216 WP_013694234.1 hypothetical protein -
  PSH71_RS25795 (PSH71_25790) - 5790351..5791037 (-) 687 WP_003205678.1 energy-coupling factor ABC transporter permease -
  PSH71_RS25800 (PSH71_25795) - 5791079..5791489 (-) 411 WP_003205679.1 tautomerase family protein -
  PSH71_RS25805 (PSH71_25800) - 5791641..5792534 (+) 894 WP_003205681.1 LysR substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44246.00 Da        Isoelectric Point: 10.0786

>NTDB_id=785620 PSH71_RS25770 WP_003205670.1 5787147..5788364(+) (pilC) [Pseudomonas brassicacearum strain FP1843]
MAVKAVKTDVYTWEGKDRKGTKMSGELTGQSPALVKAQLRKQGINPQKVRKKSTSIFSKGKRIKPLDIALFTRQMATMLK
AGVPLLQAFDIIGEGFDNPNMRKLVDEVKQEVAAGNSFAAALRKCPQYFDELYCNLVDAGEQAGALDTLLDRVATYKEKS
EALKAKIKKAMTYPAAVVLVAAVVTGILLVKVVPQFESVFSGFGAKLPAFTVMVIGLSEIMQQWWWVMLGGAVGAFFGVK
YALRRSERFRDWRDKWLLKLPLIGTLLYKSAVARYARTLSTTFAAGVPLVEALDSVSGATGNVVFKRAVQRIKQEVSTGM
QLNFSMRASGIFPNLAVQMTAIGEESGALDDMLDKVASFYEAEVDNLVDNLTSLMEPFIMVVLGVVVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=785620 PSH71_RS25770 WP_003205670.1 5787147..5788364(+) (pilC) [Pseudomonas brassicacearum strain FP1843]
ATGGCGGTCAAGGCAGTCAAAACCGATGTTTACACTTGGGAAGGCAAGGACCGCAAAGGCACGAAAATGAGTGGCGAGCT
GACGGGTCAAAGCCCGGCGCTGGTCAAGGCACAGCTGCGCAAACAAGGCATCAACCCGCAGAAGGTGCGCAAGAAATCCA
CCTCGATATTCAGCAAGGGCAAGCGCATCAAGCCGCTGGATATCGCCCTCTTCACCCGCCAGATGGCCACCATGCTCAAG
GCTGGTGTGCCGCTGTTGCAGGCGTTCGACATCATTGGCGAAGGCTTCGACAACCCCAACATGCGCAAGCTGGTGGACGA
GGTGAAGCAGGAAGTCGCCGCTGGCAACAGCTTCGCCGCGGCGCTGCGCAAGTGCCCGCAATATTTCGACGAGCTGTACT
GCAACCTGGTAGACGCCGGCGAGCAGGCCGGTGCCCTGGACACGCTGCTGGACCGGGTCGCGACCTACAAGGAAAAGAGC
GAAGCGCTCAAGGCCAAGATCAAGAAAGCCATGACCTATCCAGCGGCCGTGGTCCTCGTCGCCGCAGTGGTCACGGGGAT
CCTGCTGGTCAAGGTCGTGCCGCAATTCGAATCGGTATTCTCCGGGTTCGGCGCGAAACTGCCAGCCTTCACGGTGATGG
TCATAGGCCTGTCGGAAATCATGCAGCAATGGTGGTGGGTGATGCTTGGCGGGGCGGTGGGGGCTTTTTTTGGCGTGAAG
TATGCGCTCAGGCGTTCGGAGCGCTTTCGCGACTGGCGCGACAAATGGCTGCTCAAGCTGCCGCTGATCGGCACCTTGCT
GTACAAATCCGCCGTGGCCCGCTACGCCCGCACGCTATCCACTACCTTCGCCGCCGGCGTGCCGCTGGTGGAGGCTCTGG
ATTCGGTGTCTGGCGCCACCGGCAACGTGGTATTCAAACGTGCCGTGCAGCGCATCAAGCAGGAGGTCTCGACCGGCATG
CAGTTGAATTTCTCCATGCGTGCCTCAGGGATTTTTCCGAACCTGGCGGTCCAGATGACCGCCATCGGCGAGGAATCCGG
TGCGCTGGACGACATGCTCGACAAGGTGGCGAGCTTTTATGAGGCCGAGGTGGACAATCTGGTGGACAACCTCACCAGCC
TGATGGAACCCTTCATCATGGTGGTCCTGGGGGTGGTCGTCGGTGGCCTGGTAGTTGCCATGTACCTGCCCATCTTTCAA
CTCGGCTCTGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

75.556

100

0.756
  pilC Acinetobacter baylyi ADP1

58.853

99.012

0.583
  pilC Acinetobacter baumannii D1279779

57.598

100

0.58
  pilC Legionella pneumophila strain ERS1305867

54.293

97.778

0.531
  pilC Vibrio campbellii strain DS40M4

44.192

97.778

0.432
  pilG Neisseria meningitidis 44/76-A

42.469

100

0.425
  pilC Vibrio cholerae strain A1552

43.434

97.778

0.425
  pilG Neisseria gonorrhoeae MS11

42.537

99.259

0.422
  pilC Thermus thermophilus HB27

37.406

99.012

0.37