Detailed information NTG0782692

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PQV96_RS04660
• Genome accession: NZ_CP117193
• Coordinates: 995351..996484 (-)
• Length: 377 a.a.
• NCBI ID: WP_273624734.1
• Uniprot ID: -
• Organism: Acidovorax temperans strain LMJ
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 990351..1001484

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PQV96_RS04635 (PQV96_04635)	-	990698..991129 (+)	432	WP_198846181.1	Hsp20/alpha crystallin family protein	-
PQV96_RS04640 (PQV96_04640)	-	991139..991813 (-)	675	WP_207374615.1	ABC transporter ATP-binding protein	-
PQV96_RS04645 (PQV96_04645)	miaA	991824..992834 (-)	1011	WP_273624732.1	tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA	-
PQV96_RS04650 (PQV96_04650)	-	992824..994068 (-)	1245	WP_273624733.1	multidrug effflux MFS transporter	-
PQV96_RS04655 (PQV96_04655)	-	994137..994976 (-)	840	WP_273625223.1	alpha/beta hydrolase	-
PQV96_RS04660 (PQV96_04660)	pilU	995351..996484 (-)	1134	WP_273624734.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PQV96_RS04665 (PQV96_04665)	gdhA	996647..997990 (-)	1344	WP_273624735.1	NADP-specific glutamate dehydrogenase	-
PQV96_RS04670 (PQV96_04670)	-	998431..999600 (+)	1170	WP_273624736.1	glycerophosphodiester phosphodiesterase	-

Sequence

Protein

• Length: 377 a.a.
• Molecular weight: 41536.87 Da
• Isoelectric Point: 6.1853

>NTDB_id=782692 PQV96_RS04660 WP_273624734.1 995351..996484(-) (pilU) [Acidovorax temperans strain LMJ]
MEIFSFLNLIVEKAGSDLFFSVGAPVNLKIEGITHPLKMPALRPGQVKQLAYSVMNERQISEFEAKMEMNLSISAENLGR
FRVNVFVQRGETGMVIRYIKNKIPPLAALGLPPVLEKLVMRKRGLVLVTGATGSGKSTTLASMLNYRNENATGHILTIED
PLEFLHAHKLSVVDQREVGIDTHSYEEALKNALREAPDVIMIGEIRDRNTMKQAIAYAETGHLCLSTLHANNANQAMERV
INFFPEEAHRQLLVDLSLNLAGVVSQRLIPGLHEKLVPAVEVMLNSSYISDLIAKGEFSGIKEAMARSTEIGMCTFDQAL
YQLYTEDRISLDEALHNADSRTDLALRIRLAAGVSTQDVGDLSIDTSSPIQSAARLA

Nucleotide

• Length: 1134 bp

>NTDB_id=782692 PQV96_RS04660 WP_273624734.1 995351..996484(-) (pilU) [Acidovorax temperans strain LMJ]
GTGGAAATTTTCAGCTTCCTCAATCTCATAGTCGAGAAGGCAGGCTCTGATCTGTTCTTCAGTGTGGGGGCCCCTGTCAA
CTTGAAGATCGAGGGCATTACCCACCCGCTGAAAATGCCCGCGCTGCGCCCGGGGCAGGTCAAGCAACTAGCCTATTCGG
TGATGAACGAGCGCCAGATCAGCGAGTTCGAAGCCAAGATGGAGATGAATCTCTCGATCTCGGCCGAAAACCTGGGGCGC
TTCCGGGTGAACGTGTTCGTGCAGCGCGGCGAGACCGGCATGGTGATTCGCTACATCAAGAACAAGATCCCGCCCCTGGC
CGCCCTGGGCCTGCCGCCTGTTCTGGAAAAGCTGGTGATGCGCAAGCGCGGCCTGGTGCTGGTGACGGGCGCCACGGGTT
CGGGCAAATCGACCACGCTGGCGTCCATGCTCAACTACCGCAACGAAAACGCCACGGGCCACATCCTCACCATTGAAGAC
CCGCTGGAGTTTCTGCACGCCCACAAGCTCTCGGTGGTAGACCAGCGCGAGGTGGGCATTGACACCCATTCCTACGAAGA
AGCCCTGAAAAACGCCCTGCGCGAGGCGCCCGACGTGATCATGATCGGCGAAATCCGCGACCGCAACACCATGAAGCAGG
CCATCGCCTACGCCGAAACGGGGCACCTGTGCCTGTCCACACTGCACGCCAACAATGCCAACCAGGCGATGGAACGCGTG
ATCAACTTCTTCCCCGAAGAAGCGCACCGGCAACTGCTGGTAGACCTCAGCCTGAACCTGGCGGGCGTGGTGTCGCAGCG
GCTGATTCCCGGCCTGCACGAAAAGCTGGTACCTGCGGTGGAAGTGATGCTCAACTCTTCCTACATCTCCGACCTGATCG
CCAAAGGCGAGTTCTCGGGCATCAAAGAAGCGATGGCACGCAGCACCGAGATTGGCATGTGCACCTTTGACCAGGCGCTG
TACCAGCTCTACACCGAAGACCGTATTTCGCTGGACGAAGCGCTGCACAACGCCGACTCGCGCACCGACCTGGCATTGCG
CATACGCCTGGCAGCGGGGGTCTCGACGCAAGATGTCGGCGATCTGTCCATCGACACGTCCTCGCCCATCCAGTCGGCTG
CACGGTTGGCTTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (37-269)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	53.541	93.634	0.501
pilU	Vibrio cholerae strain A1552	51.695	93.899	0.485
pilU	Acinetobacter baylyi ADP1	50.992	93.634	0.477
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.081	98.143	0.403
pilT	Acinetobacter baylyi ADP1	41.018	88.594	0.363
pilT	Acinetobacter baumannii D1279779	41.515	87.533	0.363
pilT	Acinetobacter nosocomialis M2	41.515	87.533	0.363
pilT	Acinetobacter baumannii strain A118	41.515	87.533	0.363
pilT	Pseudomonas stutzeri DSM 10701	40.237	89.655	0.361
pilT	Pseudomonas aeruginosa PAK	40.237	89.655	0.361