Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   JLF49_RS03420 Genome accession   NZ_AP022473
Coordinates   730695..731105 (+) Length   136 a.a.
NCBI ID   WP_200929066.1    Uniprot ID   -
Organism   Pseudomonas monteilii strain STW0522-72     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 725695..736105
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JLF49_RS03390 (STW0522PSE72_06500) - 726232..726663 (-) 432 WP_060500126.1 hypothetical protein -
  JLF49_RS03395 (STW0522PSE72_06510) - 726750..727457 (+) 708 WP_200929064.1 energy-coupling factor ABC transporter permease -
  JLF49_RS03400 (STW0522PSE72_06530) yacG 727581..727781 (-) 201 WP_016484797.1 DNA gyrase inhibitor YacG -
  JLF49_RS03405 (STW0522PSE72_06540) coaE 727778..728401 (-) 624 WP_016713386.1 dephospho-CoA kinase -
  JLF49_RS03410 (STW0522PSE72_06550) pilD 728401..729267 (-) 867 WP_104833507.1 prepilin peptidase Machinery gene
  JLF49_RS03415 (STW0522PSE72_06560) - 729270..730478 (-) 1209 WP_200929065.1 type II secretion system F family protein -
  JLF49_RS03420 (STW0522PSE72_06570) pilA 730695..731105 (+) 411 WP_200929066.1 pilin Machinery gene
  JLF49_RS03430 (STW0522PSE72_06580) - 731418..732635 (+) 1218 WP_200929067.1 tyrosine-type recombinase/integrase -
  JLF49_RS03435 (STW0522PSE72_06590) - 732632..733315 (+) 684 WP_200929068.1 hypothetical protein -
  JLF49_RS03440 - 733334..734490 (+) 1157 WP_200928561.1 IS3 family transposase -
  JLF49_RS03445 - 734745..734948 (+) 204 WP_081502512.1 helix-turn-helix transcriptional regulator -
  JLF49_RS03450 (STW0522PSE72_06620) trfA 734951..735856 (+) 906 WP_200929069.1 plasmid replication initiator TrfA -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 13832.12 Da        Isoelectric Point: 7.6738

>NTDB_id=78230 JLF49_RS03420 WP_200929066.1 730695..731105(+) (pilA) [Pseudomonas monteilii strain STW0522-72]
MKGQRGITLIELMIVVAIIGILATIALPMYTNHQARSKAAAGLLEISALKTAMDLRLNDGKEVGGVAELGGRSATTHCAI
TAEGKAADGSGKIACTLVDAPANVVGKALTLTRSASGWVCTTDIEEDLAPKGCKGA

Nucleotide


Download         Length: 411 bp        

>NTDB_id=78230 JLF49_RS03420 WP_200929066.1 730695..731105(+) (pilA) [Pseudomonas monteilii strain STW0522-72]
ATGAAAGGGCAACGCGGTATCACCCTGATCGAACTGATGATCGTTGTCGCGATCATCGGCATTCTGGCGACCATCGCCTT
ACCGATGTACACCAACCACCAGGCACGCTCCAAGGCGGCGGCCGGGTTGCTGGAAATCAGCGCGCTGAAGACAGCGATGG
ACCTGCGCCTGAATGACGGGAAAGAAGTGGGGGGCGTGGCCGAGCTGGGTGGCCGATCTGCCACGACGCATTGTGCGATC
ACCGCCGAAGGCAAGGCGGCGGATGGCAGCGGGAAGATTGCTTGCACGCTGGTGGATGCACCGGCCAATGTGGTGGGTAA
GGCACTGACCCTGACTCGCTCGGCCAGTGGGTGGGTGTGCACTACTGACATCGAGGAAGACCTGGCGCCCAAGGGTTGCA
AGGGGGCTTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-134)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

43.137

100

0.485
  pilA Acinetobacter baumannii strain A118

44.526

100

0.449
  comP Acinetobacter baylyi ADP1

39.735

100

0.441
  pilA Vibrio cholerae C6706

39.073

100

0.434
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

39.073

100

0.434
  pilA Vibrio cholerae strain A1552

39.073

100

0.434
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.912

100

0.419
  pilA/pilAII Pseudomonas stutzeri DSM 10701

41.481

99.265

0.412
  pilA Vibrio parahaemolyticus RIMD 2210633

40.164

89.706

0.36

Multiple sequence alignment