Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LYZ37_RS02185 Genome accession   NZ_CP117029
Coordinates   447229..448920 (-) Length   563 a.a.
NCBI ID   WP_272787133.1    Uniprot ID   -
Organism   Vibrio tubiashii strain FP17     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 442229..453920
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LYZ37_RS02155 (LYZ37_02155) rplS 442699..443052 (+) 354 WP_004742596.1 50S ribosomal protein L19 -
  LYZ37_RS02160 (LYZ37_02160) yacG 443425..443619 (-) 195 WP_272786274.1 DNA gyrase inhibitor YacG -
  LYZ37_RS02165 (LYZ37_02165) zapD 443682..444422 (-) 741 WP_272786275.1 cell division protein ZapD -
  LYZ37_RS02170 (LYZ37_02170) coaE 444457..445065 (-) 609 WP_272786276.1 dephospho-CoA kinase -
  LYZ37_RS02175 (LYZ37_02175) pilD 445067..445936 (-) 870 WP_272786277.1 prepilin peptidase Machinery gene
  LYZ37_RS02180 (LYZ37_02180) pilC 445995..447218 (-) 1224 WP_272786278.1 type II secretion system F family protein Machinery gene
  LYZ37_RS02185 (LYZ37_02185) pilB 447229..448920 (-) 1692 WP_272787133.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LYZ37_RS02190 (LYZ37_02190) pilA 448920..449324 (-) 405 WP_272786279.1 pilin Machinery gene
  LYZ37_RS02195 (LYZ37_02195) nadC 449566..450453 (-) 888 WP_272786280.1 carboxylating nicotinate-nucleotide diphosphorylase -
  LYZ37_RS02200 (LYZ37_02200) ampD 450585..451130 (+) 546 WP_272786281.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  LYZ37_RS02205 (LYZ37_02205) fldB 451311..451832 (-) 522 WP_272786282.1 flavodoxin FldB -
  LYZ37_RS02210 (LYZ37_02210) xerD 451996..452892 (+) 897 WP_004742607.1 site-specific tyrosine recombinase XerD -
  LYZ37_RS02215 (LYZ37_02215) - 452933..453685 (+) 753 WP_272786283.1 thioredoxin fold domain-containing protein -

Sequence

Protein


Download         Length: 563 a.a.        Molecular weight: 62288.31 Da        Isoelectric Point: 5.0922

>NTDB_id=782101 LYZ37_RS02185 WP_272787133.1 447229..448920(-) (pilB) [Vibrio tubiashii strain FP17]
MLTNLPSILRQAELLDPVQEAQLVELVKDTNLNVAEVIVEQGWLTQAELVSQLSQIFALPIIDFANYDYQTVCHSLGLRE
LITRSRAVPILQSEHQLLIAVTDPTNIAIEEEFRFATGKQIELVLADVKQLNGAVRRLYGSAICGEEASHAKELSEQELV
SLVELSNDEINPVEDLSSDNSPVSRYINQILLDAVRKKASDIHFEPYEASYRVRFRCDGILIETQQPSHTLSRRLSARLK
IIAKLDIAERRLPQDGRIKLQLSASTAVDIRISTLPTLWGEKIVLRLLDSSSAELDIQSLGYNQHQQALYQKALSKPQGM
ILMTGPTGSGKTVSLYAGLNMINTPQVNIATAEDPVEINLAGVNQVQINPKIGLDFAQALRAFLRQDPDIVMVGEIRDLE
TAEIAVKAAQTGHLVLSTLHTNSAAESVVRLQHMGISPFNVSSSLSLIIAQRLARKLCNHCKQQQTLPETHCQQLELANN
TVVFAANEQGCSECSQGYFGRTGIYEVMQIDDKLVQVINQEADAKAIEKVAIKQGMQTLKESGTEKLLAGVTSYLELQRV
LYL

Nucleotide


Download         Length: 1692 bp        

>NTDB_id=782101 LYZ37_RS02185 WP_272787133.1 447229..448920(-) (pilB) [Vibrio tubiashii strain FP17]
GTGCTAACCAACCTGCCCTCTATTTTGCGCCAAGCTGAGCTTCTTGACCCTGTTCAAGAAGCTCAGTTGGTTGAATTGGT
TAAGGACACAAATCTTAACGTGGCCGAAGTAATAGTAGAGCAAGGTTGGCTCACCCAAGCAGAATTAGTCTCACAACTCT
CGCAAATTTTTGCCTTACCCATCATCGACTTCGCCAATTACGATTATCAAACGGTATGCCACAGTTTAGGCCTGCGCGAA
CTGATCACCCGCTCCCGAGCAGTGCCTATTCTACAGAGTGAACACCAACTGCTTATCGCCGTTACCGACCCGACCAATAT
CGCAATCGAAGAAGAGTTTCGCTTTGCGACCGGAAAGCAGATTGAGTTAGTTTTAGCTGACGTAAAGCAGCTCAATGGCG
CGGTACGTCGCTTGTATGGCAGCGCTATCTGTGGTGAAGAAGCGAGTCATGCAAAAGAGCTGAGCGAACAAGAGCTCGTC
AGCTTAGTCGAGCTCTCTAATGATGAGATTAACCCAGTCGAAGACCTGAGTAGTGATAACTCACCGGTAAGTCGGTATAT
CAATCAAATATTACTGGATGCGGTTAGGAAAAAGGCATCTGACATTCACTTTGAACCCTATGAAGCCTCTTATCGTGTGC
GATTTCGTTGTGATGGCATTTTGATTGAGACTCAACAACCTAGCCATACATTAAGTCGCCGTTTATCGGCAAGACTAAAG
ATCATCGCCAAACTCGATATTGCCGAGCGCCGCTTACCTCAAGATGGACGCATCAAGCTTCAACTCAGTGCAAGTACCGC
TGTCGATATTCGTATTTCAACCCTACCAACTCTATGGGGCGAAAAGATCGTGCTACGCCTTCTCGATAGCAGCAGTGCCG
AGTTAGACATTCAATCACTAGGGTACAACCAACACCAACAAGCTTTGTATCAAAAAGCACTGAGCAAACCCCAAGGCATG
ATTTTGATGACAGGACCAACAGGCAGCGGTAAGACAGTCTCACTTTATGCGGGGTTAAACATGATCAATACCCCACAAGT
GAACATCGCCACCGCAGAAGACCCGGTGGAAATTAATTTAGCGGGCGTTAACCAAGTTCAAATCAACCCCAAAATTGGAT
TAGACTTCGCCCAAGCCTTGCGCGCCTTCCTACGCCAAGACCCGGACATTGTCATGGTCGGTGAGATACGCGACCTCGAA
ACGGCTGAAATCGCAGTCAAAGCGGCACAAACAGGTCACCTAGTGCTATCAACATTGCATACGAACTCGGCTGCCGAAAG
CGTCGTTCGCTTGCAACATATGGGCATCTCCCCATTCAATGTCTCTTCATCACTGAGCTTAATCATTGCCCAGCGTTTGG
CACGAAAGCTGTGTAACCATTGTAAGCAGCAGCAAACCTTGCCAGAGACACATTGCCAACAACTGGAGTTAGCAAACAAC
ACCGTGGTTTTTGCTGCTAATGAACAAGGGTGCAGTGAATGCAGTCAAGGCTATTTTGGGCGAACTGGAATCTATGAAGT
GATGCAGATAGACGACAAGTTAGTGCAAGTCATCAATCAAGAGGCAGATGCTAAAGCCATCGAAAAGGTCGCGATAAAGC
AAGGAATGCAGACGCTTAAAGAGTCTGGAACGGAGAAACTGCTCGCAGGAGTCACCAGTTACCTAGAGTTGCAACGTGTT
CTTTACTTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(189-456)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

64.121

100

0.641
  pilB Vibrio parahaemolyticus RIMD 2210633

62.721

100

0.631
  pilB Vibrio campbellii strain DS40M4

62.345

100

0.623
  pilB Legionella pneumophila strain ERS1305867

46.237

99.112

0.458
  pilB Acinetobacter baumannii D1279779

47.826

93.961

0.449
  pilB Acinetobacter baylyi ADP1

46.041

96.448

0.444
  pilF Neisseria gonorrhoeae MS11

43.797

94.494

0.414
  pilF Thermus thermophilus HB27

38.06

95.204

0.362
  pilB Haemophilus influenzae Rd KW20

42.062

86.146

0.362
  pilB/pilB1 Synechocystis sp. PCC 6803

38.889

92.718

0.361