Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PL263_RS15860 Genome accession   NZ_CP116398
Coordinates   3535795..3537012 (+) Length   405 a.a.
NCBI ID   WP_278210268.1    Uniprot ID   -
Organism   Methylomonas sp. EFPC3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3530795..3542012
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PL263_RS15850 (PL263_15845) thiC 3532040..3533908 (+) 1869 WP_278210262.1 phosphomethylpyrimidine synthase ThiC -
  PL263_RS15855 (PL263_15850) pilB 3534077..3535792 (+) 1716 WP_140913440.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PL263_RS15860 (PL263_15855) pilC 3535795..3537012 (+) 1218 WP_278210268.1 type II secretion system F family protein Machinery gene
  PL263_RS15865 (PL263_15860) pilD 3537072..3537938 (+) 867 WP_278210270.1 A24 family peptidase Machinery gene
  PL263_RS15870 (PL263_15865) coaE 3537942..3538553 (+) 612 WP_278210271.1 dephospho-CoA kinase -
  PL263_RS15875 (PL263_15870) zapD 3538550..3539323 (+) 774 WP_140913444.1 cell division protein ZapD -
  PL263_RS15880 (PL263_15875) - 3539323..3539538 (+) 216 WP_278210275.1 DNA gyrase inhibitor YacG -
  PL263_RS15885 (PL263_15880) - 3539638..3540576 (-) 939 WP_278210276.1 Nudix family hydrolase -
  PL263_RS15890 (PL263_15885) argJ 3540585..3541799 (-) 1215 WP_278210277.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44325.31 Da        Isoelectric Point: 7.8486

>NTDB_id=777139 PL263_RS15860 WP_278210268.1 3535795..3537012(+) (pilC) [Methylomonas sp. EFPC3]
MAKQEEQIEFNWDGFDKQNRKTKGTISAKSEVIARAELRRMGIRVIGIKPKAKPLFSPRVQKITPGDVAVFARQLATMLA
AGVPLVQSFDIIGKGHDNASMSEMLLSIKADVEGGDTLAQALGRKPLYFDALFCNLVEAGEHAGVLETLLDKIATYKEKT
ESIKKKVKKALTYPVAVLVVAFIVTAILLVFVVPVFEDLFKGFGADLPAFTKFVIDLSAWVQEWWWAVVGVLFVAGYTFT
YFKKRSKAFNHFLDRTLLKIPVVGMILQKSAIARFARTLSTMSAAGVPLVEALVSVAGACGNILFYEAVMKVRDDVATGQ
QLKFALETTGIFPNMVVQMVAIGEESGSIDAMLDKVADFYEEEVDNLVDNLSSLMEPIIMVILGILVGGLIVAMYLPIFK
LGAAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=777139 PL263_RS15860 WP_278210268.1 3535795..3537012(+) (pilC) [Methylomonas sp. EFPC3]
ATGGCAAAACAAGAAGAGCAGATCGAATTCAATTGGGACGGTTTCGACAAACAAAACCGGAAAACCAAAGGCACTATCAG
TGCCAAAAGCGAAGTGATCGCTCGCGCCGAGCTGCGGCGGATGGGCATCCGGGTGATCGGTATCAAACCCAAGGCGAAAC
CCTTGTTCAGTCCGCGGGTGCAAAAAATCACGCCGGGCGATGTTGCCGTTTTTGCCCGCCAATTGGCGACCATGCTGGCG
GCCGGCGTACCGCTGGTGCAGTCGTTCGATATCATCGGCAAGGGCCACGACAACGCCAGCATGTCGGAAATGCTGTTGTC
GATCAAAGCCGACGTCGAGGGTGGCGATACCCTGGCGCAAGCATTGGGCCGCAAGCCGCTCTATTTCGACGCCTTGTTTT
GCAATCTGGTCGAAGCCGGCGAACATGCCGGGGTATTGGAAACCTTGTTGGATAAGATTGCCACTTACAAGGAAAAAACC
GAGTCGATCAAGAAAAAAGTCAAAAAGGCTCTGACCTACCCGGTGGCAGTATTGGTGGTGGCGTTTATCGTTACCGCGAT
TTTGTTGGTGTTCGTGGTGCCGGTGTTCGAGGACTTGTTTAAAGGCTTCGGCGCCGATTTGCCGGCGTTCACCAAATTCG
TCATCGATTTGTCGGCCTGGGTACAGGAATGGTGGTGGGCCGTGGTCGGCGTGCTATTTGTCGCCGGCTACACCTTTACC
TACTTTAAAAAACGCTCGAAGGCCTTCAACCATTTTCTGGATCGGACCCTGTTGAAAATACCGGTGGTCGGTATGATTTT
GCAAAAATCGGCCATCGCCCGCTTCGCCAGAACCCTCTCGACCATGTCGGCCGCCGGCGTGCCGTTGGTCGAAGCCTTGG
TTTCGGTGGCCGGCGCGTGCGGTAACATCTTGTTTTACGAGGCGGTGATGAAGGTGCGCGACGACGTCGCTACCGGCCAG
CAATTGAAGTTCGCCTTGGAAACCACCGGCATATTTCCGAATATGGTGGTACAGATGGTGGCGATCGGCGAAGAATCCGG
TTCGATCGACGCCATGCTGGACAAGGTCGCCGACTTTTACGAGGAAGAGGTCGATAACTTGGTCGACAACCTGAGCAGTT
TGATGGAACCTATCATCATGGTGATCTTGGGGATTTTGGTCGGCGGTTTGATCGTGGCGATGTATTTGCCGATCTTCAAA
TTGGGCGCCGCTATCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Legionella pneumophila strain ERS1305867

59.548

98.272

0.585
  pilC Pseudomonas stutzeri DSM 10701

56.566

97.778

0.553
  pilC Acinetobacter baumannii D1279779

53.401

98.025

0.523
  pilC Acinetobacter baylyi ADP1

51.961

100

0.523
  pilG Neisseria gonorrhoeae MS11

46.814

100

0.472
  pilG Neisseria meningitidis 44/76-A

46.814

100

0.472
  pilC Vibrio cholerae strain A1552

42.025

97.531

0.41
  pilC Vibrio campbellii strain DS40M4

40.609

97.284

0.395
  pilC Thermus thermophilus HB27

36.591

98.519

0.36