Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   PL263_RS15865 Genome accession   NZ_CP116398
Coordinates   3537072..3537938 (+) Length   288 a.a.
NCBI ID   WP_278210270.1    Uniprot ID   -
Organism   Methylomonas sp. EFPC3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3532072..3542938
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PL263_RS15855 (PL263_15850) pilB 3534077..3535792 (+) 1716 WP_140913440.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PL263_RS15860 (PL263_15855) pilC 3535795..3537012 (+) 1218 WP_278210268.1 type II secretion system F family protein Machinery gene
  PL263_RS15865 (PL263_15860) pilD 3537072..3537938 (+) 867 WP_278210270.1 A24 family peptidase Machinery gene
  PL263_RS15870 (PL263_15865) coaE 3537942..3538553 (+) 612 WP_278210271.1 dephospho-CoA kinase -
  PL263_RS15875 (PL263_15870) zapD 3538550..3539323 (+) 774 WP_140913444.1 cell division protein ZapD -
  PL263_RS15880 (PL263_15875) - 3539323..3539538 (+) 216 WP_278210275.1 DNA gyrase inhibitor YacG -
  PL263_RS15885 (PL263_15880) - 3539638..3540576 (-) 939 WP_278210276.1 Nudix family hydrolase -
  PL263_RS15890 (PL263_15885) argJ 3540585..3541799 (-) 1215 WP_278210277.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -

Sequence

Protein


Download         Length: 288 a.a.        Molecular weight: 31529.52 Da        Isoelectric Point: 7.4707

>NTDB_id=777140 PL263_RS15865 WP_278210270.1 3537072..3537938(+) (pilD) [Methylomonas sp. EFPC3]
MLFNVLAQSPGFLAASALVLGLLVGSFLNVVIYRLPLMMQAAWRRECREYLELPAEAPGERFDLLLPASHCPACKAEIKA
YQNIPVLSYVLLGGKCANCGNKIGWRYPSIEAFTGLCSALVAWQLGYGWALAFALPLTWCLITLSFIDIDQQLLPDSITL
PLVWLGLLLSVFGIFADSRDSIVGAVAGYLSLWSVYHGFKLLTGKDGMGYGDFKLLAVFGAWLGWQYLPLIILLASLVGA
VIGVAMIVFCRRDAAKPIPFGPYLAMAGWLALLWGEQMNAWYLRLAGL

Nucleotide


Download         Length: 867 bp        

>NTDB_id=777140 PL263_RS15865 WP_278210270.1 3537072..3537938(+) (pilD) [Methylomonas sp. EFPC3]
ATGTTGTTCAATGTCTTAGCTCAATCTCCCGGTTTTCTGGCCGCAAGCGCTTTAGTACTCGGCTTGCTGGTCGGCAGTTT
TCTGAATGTGGTGATTTACCGGCTGCCGCTGATGATGCAGGCTGCGTGGCGGCGCGAGTGCCGCGAATACCTGGAATTGC
CGGCCGAAGCCCCGGGCGAGCGTTTCGATCTGTTATTGCCGGCTTCGCACTGTCCGGCTTGCAAAGCCGAAATCAAAGCC
TATCAAAACATCCCGGTCCTGAGCTATGTGCTGCTCGGCGGCAAATGCGCCAACTGCGGCAACAAAATCGGTTGGCGTTA
TCCTTCGATAGAGGCCTTTACCGGCCTGTGTTCGGCGCTGGTCGCTTGGCAACTGGGTTACGGCTGGGCGCTGGCGTTTG
CGTTGCCGTTGACTTGGTGTCTGATTACACTGAGTTTTATCGATATCGATCAGCAATTGCTGCCGGACTCGATCACGCTG
CCTTTGGTCTGGCTGGGTTTGCTGCTCAGCGTGTTCGGCATTTTTGCCGACAGCCGCGACAGTATCGTCGGCGCGGTAGC
CGGTTATTTAAGCTTGTGGAGCGTCTACCACGGCTTTAAATTGCTGACCGGCAAGGACGGCATGGGTTACGGCGATTTCA
AATTACTGGCCGTATTCGGCGCCTGGCTGGGCTGGCAATATTTGCCGCTGATAATCTTGTTGGCTTCCCTGGTCGGTGCG
GTGATTGGCGTCGCGATGATCGTGTTCTGCCGGCGCGATGCGGCCAAGCCGATTCCGTTCGGGCCTTATCTGGCTATGGC
CGGCTGGCTGGCGCTATTGTGGGGTGAGCAAATGAATGCTTGGTATCTGCGGCTTGCAGGTCTGTGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(19-124)

Type IV leader peptidase family (Peptidase_A24)

(137-244)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio campbellii strain DS40M4

51.418

97.917

0.503
  pilD Acinetobacter baumannii D1279779

52.574

94.444

0.497
  pilD Acinetobacter nosocomialis M2

51.838

94.444

0.49
  pilD Vibrio cholerae strain A1552

49.814

93.403

0.465
  pilD Neisseria gonorrhoeae MS11

51.765

88.542

0.458