Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PL263_RS15855 Genome accession   NZ_CP116398
Coordinates   3534077..3535792 (+) Length   571 a.a.
NCBI ID   WP_140913440.1    Uniprot ID   -
Organism   Methylomonas sp. EFPC3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3529077..3540792
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PL263_RS15840 (PL263_15835) - 3529697..3530110 (-) 414 WP_278210259.1 hypothetical protein -
  PL263_RS15845 (PL263_15840) recD 3530110..3531747 (-) 1638 WP_278210260.1 exodeoxyribonuclease V subunit alpha -
  PL263_RS15850 (PL263_15845) thiC 3532040..3533908 (+) 1869 WP_278210262.1 phosphomethylpyrimidine synthase ThiC -
  PL263_RS15855 (PL263_15850) pilB 3534077..3535792 (+) 1716 WP_140913440.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PL263_RS15860 (PL263_15855) pilC 3535795..3537012 (+) 1218 WP_278210268.1 type II secretion system F family protein Machinery gene
  PL263_RS15865 (PL263_15860) pilD 3537072..3537938 (+) 867 WP_278210270.1 A24 family peptidase Machinery gene
  PL263_RS15870 (PL263_15865) coaE 3537942..3538553 (+) 612 WP_278210271.1 dephospho-CoA kinase -
  PL263_RS15875 (PL263_15870) zapD 3538550..3539323 (+) 774 WP_140913444.1 cell division protein ZapD -
  PL263_RS15880 (PL263_15875) - 3539323..3539538 (+) 216 WP_278210275.1 DNA gyrase inhibitor YacG -
  PL263_RS15885 (PL263_15880) - 3539638..3540576 (-) 939 WP_278210276.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63304.00 Da        Isoelectric Point: 6.1220

>NTDB_id=777138 PL263_RS15855 WP_140913440.1 3534077..3535792(+) (pilB) [Methylomonas sp. EFPC3]
MATAPSNIHFSGLVKCLIQKGLLTEESAATCLQEAQKRKQAFISYLVEHKYADSKTIATLASAEFGVPFFDLDAIDPAFL
PIKMVSEKLIRQHHALPLFKRGNRLFVATADPTNFQALDEIKFHTRHNTENILVEEDKLIRMIDTALEAADTSMKDLLDD
DLDNIDISAGDEAKPAEDAMGSDIDDAPIVRFVNKILLDSIKRGVSDIHMEPFEKRFRIRFRADGILSEVASPPVGIANR
IISRLKVMSKMDIAERRVPQDGRIKMQISKNRAIDFRVNTCPTLFGEKVVLRILDPTSAQIGIEKLGFEPEQQKNFLDAI
HKPYGMVLVTGPTGSGKTVSLYTGLNILNSVETNISTAEDPVEITVEGINQVNVNPKAGLTFAEALKAFLRQDPDIIMVG
EIRDYETASIAVKAAQTGHLVLSTLHTNDAPQTINRLVQMGIEPFNIVSAVLLIMAQRLARRLCEHCKKEMDYPESALIS
AGFKKEELGTFKIYGPVGCEHCNNGYKGRVGIYQVMPITEKMRALILRGGNAMEMAELSRSEGINDLRTSGLLKIRQGIT
SLEEIDRVTTE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=777138 PL263_RS15855 WP_140913440.1 3534077..3535792(+) (pilB) [Methylomonas sp. EFPC3]
ATGGCGACAGCACCTTCCAACATTCATTTTAGCGGTCTGGTTAAGTGCCTGATTCAGAAGGGGCTGCTGACAGAAGAGTC
GGCAGCGACCTGTTTACAGGAGGCGCAAAAGCGCAAACAGGCGTTCATTAGCTATTTGGTCGAACACAAATACGCGGATA
GCAAAACCATCGCCACGCTGGCCTCGGCCGAATTCGGCGTGCCGTTTTTCGATCTGGACGCGATCGATCCCGCGTTTCTG
CCGATCAAAATGGTCAGCGAAAAATTGATTCGCCAGCACCACGCCTTGCCCCTCTTTAAACGCGGTAACCGTTTGTTCGT
CGCGACGGCCGACCCGACCAATTTCCAGGCCCTGGACGAGATCAAGTTCCACACCCGGCACAATACCGAAAATATTCTGG
TCGAAGAAGACAAACTGATCAGAATGATAGACACGGCGCTGGAAGCCGCGGATACGTCGATGAAGGATCTGCTCGACGAC
GATCTGGACAACATCGATATTTCCGCCGGCGACGAGGCCAAGCCGGCCGAAGACGCTATGGGTTCGGATATCGACGACGC
GCCTATCGTCCGCTTCGTGAATAAGATTTTGCTGGATTCGATCAAGCGCGGCGTATCGGATATTCACATGGAACCGTTCG
AGAAGCGGTTCCGCATCCGCTTCCGCGCCGACGGCATACTGTCGGAAGTGGCGAGTCCGCCGGTCGGTATCGCTAATCGG
ATCATTTCCCGGCTGAAAGTCATGTCCAAGATGGACATCGCCGAACGGCGGGTGCCGCAGGACGGCCGGATCAAGATGCA
GATCTCGAAAAACCGCGCGATCGACTTCAGGGTCAACACCTGTCCGACCTTGTTCGGCGAAAAAGTGGTGTTACGGATTC
TGGATCCGACCAGCGCCCAGATCGGCATCGAAAAACTCGGGTTCGAACCGGAACAGCAGAAAAATTTCCTGGATGCGATT
CATAAACCCTACGGCATGGTGTTGGTCACCGGGCCGACCGGTAGCGGTAAGACGGTGTCGCTGTATACCGGTTTGAATAT
TCTGAACAGCGTCGAAACCAATATTTCCACGGCCGAAGACCCGGTAGAGATCACCGTAGAAGGCATCAACCAGGTCAACG
TCAACCCTAAAGCCGGCTTGACTTTTGCCGAAGCGCTCAAAGCATTCTTGCGGCAGGACCCGGACATCATCATGGTCGGC
GAGATCCGCGACTATGAAACCGCCAGTATCGCCGTCAAAGCAGCCCAAACCGGCCACTTGGTGTTGTCGACGCTGCACAC
CAACGACGCGCCGCAGACCATCAACCGGCTGGTACAGATGGGGATCGAGCCGTTCAATATCGTCTCGGCAGTTTTGCTGA
TTATGGCGCAACGTCTGGCACGGCGTTTGTGCGAGCATTGCAAGAAGGAAATGGATTACCCGGAAAGTGCGCTGATCTCG
GCCGGCTTCAAAAAGGAAGAACTTGGCACTTTCAAAATATACGGTCCGGTGGGCTGCGAGCATTGCAATAACGGCTACAA
AGGGCGGGTCGGTATTTACCAGGTGATGCCGATCACCGAAAAAATGCGGGCCTTGATCCTGCGCGGCGGCAATGCGATGG
AGATGGCCGAATTGTCCCGCAGCGAAGGCATCAACGATTTGCGCACCTCCGGCTTGTTGAAAATCCGCCAGGGCATCACC
TCACTGGAAGAAATTGACCGCGTCACCACGGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(195-462)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

54.957

100

0.553
  pilB Acinetobacter baylyi ADP1

53.369

98.774

0.527
  pilB Acinetobacter baumannii D1279779

52.356

100

0.525
  pilF Neisseria gonorrhoeae MS11

48.029

97.723

0.469
  pilB Vibrio parahaemolyticus RIMD 2210633

45.855

99.299

0.455
  pilB Vibrio campbellii strain DS40M4

45.487

98.949

0.45
  pilB Vibrio cholerae strain A1552

49.803

88.967

0.443
  pilF Thermus thermophilus HB27

39.716

98.774

0.392
  pilB/pilB1 Synechocystis sp. PCC 6803

39.709

96.147

0.382
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.104

97.898

0.373