Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PI990_RS05930 Genome accession   NZ_CP116239
Coordinates   1253288..1254505 (-) Length   405 a.a.
NCBI ID   WP_271660081.1    Uniprot ID   -
Organism   Pseudomonas sp. TUM22785     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1248288..1259505
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PI990_RS05885 (PI990_05885) - 1248708..1248881 (-) 174 WP_021221826.1 DUF3094 family protein -
  PI990_RS05890 (PI990_05890) - 1248968..1249594 (-) 627 WP_021221827.1 DUF1780 domain-containing protein -
  PI990_RS05895 (PI990_05895) - 1249708..1250133 (+) 426 WP_271660076.1 GNAT family N-acetyltransferase -
  PI990_RS05900 (PI990_05900) - 1250130..1250597 (+) 468 WP_271660077.1 FAD/FMN-containing dehydrogenase -
  PI990_RS05905 (PI990_05905) - 1250594..1251280 (+) 687 WP_271660078.1 energy-coupling factor ABC transporter permease -
  PI990_RS05910 (PI990_05910) - 1251388..1251603 (+) 216 WP_043244210.1 hypothetical protein -
  PI990_RS05915 (PI990_05915) yacG 1251607..1251807 (-) 201 WP_021221832.1 DNA gyrase inhibitor YacG -
  PI990_RS05920 (PI990_05920) coaE 1251804..1252415 (-) 612 WP_271660079.1 dephospho-CoA kinase -
  PI990_RS05925 (PI990_05925) pilD 1252415..1253284 (-) 870 WP_271660080.1 A24 family peptidase Machinery gene
  PI990_RS05930 (PI990_05930) pilC 1253288..1254505 (-) 1218 WP_271660081.1 type II secretion system F family protein Machinery gene
  PI990_RS05935 (PI990_05935) pilB 1254509..1256212 (-) 1704 WP_271660082.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PI990_RS05940 (PI990_05940) - 1256437..1256934 (+) 498 WP_271660083.1 pilin -
  PI990_RS05945 (PI990_05945) - 1257041..1258726 (+) 1686 WP_271660084.1 O-antigen ligase family protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44316.15 Da        Isoelectric Point: 9.7212

>NTDB_id=776323 PI990_RS05930 WP_271660081.1 1253288..1254505(-) (pilC) [Pseudomonas sp. TUM22785]
MAEKAVKTSVFKWEGTDKKGTKVTGELSGINPSLIKAQLRKQGINPLKVRKKAVSLFGSGKKIKPLDIALFTRQMATMMK
SGVPLLQSFDIIGEGFDNQNMRKLVDDVKQDVAAGNSFAASLRKRPLYFDDLYCNLVDSGEQSGALETLLDRVATYKEKT
EALKSKIKKAMNYPIAVVLVAVIVSAILLIKVVPQFQSVFANFGAELPAFTLMVIGLSEALQEWWYIILFAFIGIFFGLK
QAHQRSEEFRNWLDRFVLKLPIVGDILYKAVVARFARTLATTFAAGVPLVDALDSVAGATGNIVFKNAVARIKQDVSSGM
QLNFSMRTTGVFPSMAIQMTAIGEESGSLDLMLDKVATYYEQEVDNAVDGLTALMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGSVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=776323 PI990_RS05930 WP_271660081.1 1253288..1254505(-) (pilC) [Pseudomonas sp. TUM22785]
ATGGCGGAAAAAGCAGTAAAAACGAGTGTCTTCAAATGGGAAGGCACCGATAAGAAAGGCACCAAGGTCACCGGTGAGCT
CAGCGGTATCAACCCCTCGCTGATCAAAGCCCAACTGCGCAAGCAAGGCATCAACCCGCTCAAAGTGCGAAAGAAGGCAG
TATCGCTCTTTGGCTCGGGAAAAAAGATCAAGCCCTTGGATATCGCACTTTTCACTCGCCAGATGGCCACCATGATGAAG
TCCGGTGTTCCTCTCTTGCAGTCTTTCGACATCATTGGCGAGGGGTTCGACAACCAGAACATGCGCAAGCTGGTCGACGA
CGTCAAGCAGGATGTCGCAGCGGGTAATAGCTTCGCCGCTTCCCTGCGCAAGAGACCTCTTTATTTCGATGACCTTTACT
GCAACTTGGTGGATTCGGGCGAACAGTCGGGCGCACTCGAAACGCTGCTTGATCGAGTGGCCACCTATAAGGAAAAGACC
GAGGCGCTGAAGTCCAAGATCAAGAAGGCGATGAACTACCCAATCGCTGTAGTGTTGGTTGCGGTTATAGTCTCTGCCAT
TTTGCTTATCAAAGTCGTTCCGCAGTTCCAGTCTGTTTTCGCAAACTTCGGTGCCGAGCTTCCTGCCTTTACTCTAATGG
TCATAGGTCTATCCGAGGCCCTGCAAGAGTGGTGGTACATAATACTATTCGCGTTCATAGGTATATTTTTCGGACTCAAG
CAAGCTCACCAGCGTTCAGAAGAATTTCGCAACTGGCTGGATCGGTTCGTCCTCAAGCTTCCCATTGTGGGTGACATCCT
CTACAAGGCCGTAGTTGCACGTTTCGCCCGAACACTCGCTACCACATTCGCCGCAGGCGTTCCGCTGGTGGATGCCCTTG
ATTCAGTGGCTGGCGCTACTGGCAATATCGTATTCAAGAATGCCGTTGCCAGAATCAAGCAAGACGTCTCGTCCGGCATG
CAACTGAACTTCTCCATGCGTACCACTGGCGTATTCCCGAGCATGGCTATTCAGATGACGGCTATCGGTGAGGAGTCAGG
CTCTCTGGACCTGATGCTGGACAAGGTCGCAACATATTATGAGCAGGAAGTAGACAACGCCGTAGACGGTCTCACGGCCC
TGATGGAGCCCATGATCATGGCGGTCCTCGGTGTCCTGGTGGGCGGTTTGATCATCGCCATGTACCTGCCCATCTTCCAA
CTCGGTTCCGTGGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

83.21

100

0.832
  pilC Acinetobacter baylyi ADP1

59.606

100

0.598
  pilC Acinetobacter baumannii D1279779

59.259

100

0.593
  pilC Legionella pneumophila strain ERS1305867

54.545

97.778

0.533
  pilG Neisseria gonorrhoeae MS11

44.03

99.259

0.437
  pilG Neisseria meningitidis 44/76-A

43.781

99.259

0.435
  pilC Vibrio cholerae strain A1552

42.929

97.778

0.42
  pilC Vibrio campbellii strain DS40M4

41.667

97.778

0.407
  pilC Thermus thermophilus HB27

36.658

99.012

0.363