Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PI990_RS05935 Genome accession   NZ_CP116239
Coordinates   1254509..1256212 (-) Length   567 a.a.
NCBI ID   WP_271660082.1    Uniprot ID   -
Organism   Pseudomonas sp. TUM22785     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1249509..1261212
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PI990_RS05895 (PI990_05895) - 1249708..1250133 (+) 426 WP_271660076.1 GNAT family N-acetyltransferase -
  PI990_RS05900 (PI990_05900) - 1250130..1250597 (+) 468 WP_271660077.1 FAD/FMN-containing dehydrogenase -
  PI990_RS05905 (PI990_05905) - 1250594..1251280 (+) 687 WP_271660078.1 energy-coupling factor ABC transporter permease -
  PI990_RS05910 (PI990_05910) - 1251388..1251603 (+) 216 WP_043244210.1 hypothetical protein -
  PI990_RS05915 (PI990_05915) yacG 1251607..1251807 (-) 201 WP_021221832.1 DNA gyrase inhibitor YacG -
  PI990_RS05920 (PI990_05920) coaE 1251804..1252415 (-) 612 WP_271660079.1 dephospho-CoA kinase -
  PI990_RS05925 (PI990_05925) pilD 1252415..1253284 (-) 870 WP_271660080.1 A24 family peptidase Machinery gene
  PI990_RS05930 (PI990_05930) pilC 1253288..1254505 (-) 1218 WP_271660081.1 type II secretion system F family protein Machinery gene
  PI990_RS05935 (PI990_05935) pilB 1254509..1256212 (-) 1704 WP_271660082.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PI990_RS05940 (PI990_05940) - 1256437..1256934 (+) 498 WP_271660083.1 pilin -
  PI990_RS05945 (PI990_05945) - 1257041..1258726 (+) 1686 WP_271660084.1 O-antigen ligase family protein -
  PI990_RS05955 (PI990_05955) nadC 1258939..1259787 (-) 849 WP_271660085.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62293.25 Da        Isoelectric Point: 5.6049

>NTDB_id=776324 PI990_RS05935 WP_271660082.1 1254509..1256212(-) (pilB) [Pseudomonas sp. TUM22785]
MSESPTLSGLARQLVHANLLDEKAAQSAFIQAQRNKISLVTYLAQNKLLKSRDLAELAAEQFGIAFMDLASIDKDSQPKD
LVAEKLLRQHRALPLWRRGNKLFIAVSDPTNHQATTDIQFSTGLTTEAILVEDDKLGAALDKFFEGDASGMEDLADVDLD
GLDVEAVDDDKPSSDGGTDADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKSYRVRLRTDGILHEVARPPIQLASRISAR
LKVMAALDISERRKPQDGRIKMKISKTKSIDFRVNSLPTLWGEKIVMRILDPSSAQMGIDALGYEERQKELYLSALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPKQGMDFAQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCNSCKKEVEVPHDTLLKEGFP
EDKIGTFKIFAPTGCENCKGGYKGRVGIYEVVKNTSSLQRIIMEEGNSIDIAAQMRKDGFNDLRTSGLLKAMQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=776324 PI990_RS05935 WP_271660082.1 1254509..1256212(-) (pilB) [Pseudomonas sp. TUM22785]
ATGAGCGAATCGCCCACACTTTCAGGCTTGGCTCGGCAGTTGGTACACGCCAACCTGCTGGACGAAAAAGCTGCGCAAAG
CGCATTTATCCAGGCCCAACGCAATAAAATCTCCCTCGTCACCTACCTCGCTCAGAACAAGCTCCTGAAGAGTCGCGACC
TGGCCGAGCTGGCTGCGGAGCAGTTCGGCATTGCCTTCATGGACCTGGCAAGCATCGACAAGGACTCTCAGCCGAAAGAC
CTTGTGGCAGAGAAGCTCCTGCGCCAGCACCGCGCCCTGCCCCTCTGGCGACGTGGCAACAAGCTGTTCATTGCGGTCTC
CGACCCAACGAACCACCAAGCGACTACTGACATACAGTTCAGCACCGGCCTGACCACCGAAGCGATACTCGTCGAAGATG
ACAAGCTTGGCGCCGCACTCGACAAGTTCTTTGAGGGCGACGCTTCCGGTATGGAAGACCTGGCCGACGTCGATCTCGAT
GGGCTGGATGTTGAAGCCGTCGATGATGACAAGCCCAGCTCCGATGGCGGAACGGACGCCGACGACGCCCCCGTGGTGCG
CTTCGTCAACAAGATGCTGCTGGATGCCATCAAGGGAGGCTCATCCGACCTCCATTTCGAGCCTTATGAAAAAAGCTACC
GGGTACGCCTGCGTACCGACGGCATCCTCCATGAGGTGGCCCGCCCGCCCATCCAGCTTGCCAGCCGAATTTCGGCCCGC
CTGAAAGTCATGGCCGCACTCGACATCTCCGAACGCCGCAAGCCCCAGGACGGCCGGATCAAGATGAAGATATCCAAGAC
CAAGTCCATCGACTTTCGTGTCAACTCGCTTCCCACCCTATGGGGCGAGAAGATTGTCATGCGGATCCTGGACCCTTCGA
GCGCCCAGATGGGTATTGATGCATTGGGGTATGAAGAGCGCCAGAAGGAGCTTTACCTGAGCGCACTGAAGCAACCCCAG
GGCATGATCCTGGTAACAGGGCCGACCGGTTCGGGCAAGACGGTGTCGCTCTATACCGGCCTCAACATCCTGAATACGGT
CGATATCAACATTTCCACGGCAGAAGACCCGGTGGAAATCAACCTGGAGGGGATCAACCAGGTCAACGTCAATCCCAAGC
AGGGCATGGATTTCGCCCAGGCGCTGCGGGCGTTCTTGCGTCAGGACCCTGATGTGATCATGGTCGGCGAGATCCGCGAC
CTGGAAACCGCCGAGATCGCCATCAAGGCCGCCCAGACCGGCCACATGGTCATGTCGACCCTGCACACCAACAGTGCTGC
AGAGACCCTTACCCGACTGCGCAACATGGGGGTGCCAGCGTTCAACATCGCCACCTCCGTCAACCTGATCATCGCCCAGC
GCCTTGCCCGCAAGCTGTGCAACAGCTGCAAGAAAGAGGTGGAAGTCCCTCACGACACGCTACTGAAAGAAGGGTTCCCC
GAGGACAAGATCGGCACATTCAAGATCTTCGCCCCCACTGGCTGCGAAAACTGCAAGGGTGGCTACAAGGGCAGGGTCGG
TATTTATGAAGTAGTTAAAAACACGTCCTCTCTGCAACGCATTATCATGGAGGAAGGCAACTCCATCGATATCGCCGCGC
AGATGCGCAAAGATGGCTTCAACGATCTGCGCACCTCGGGCCTACTGAAGGCAATGCAAGGCGTCACCAGCCTCGAGGAA
GTCAACCGCGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.649

100

0.6
  pilB Acinetobacter baylyi ADP1

58.464

100

0.591
  pilB Legionella pneumophila strain ERS1305867

54.912

100

0.552
  pilB Vibrio parahaemolyticus RIMD 2210633

50.712

99.118

0.503
  pilB Vibrio cholerae strain A1552

50.356

99.118

0.499
  pilB Vibrio campbellii strain DS40M4

49.474

100

0.497
  pilF Neisseria gonorrhoeae MS11

49.378

99.295

0.49
  pilF Thermus thermophilus HB27

37.234

99.471

0.37
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.752

93.298

0.362