Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   PGA57_RS03300 Genome accession   NZ_CP116016
Coordinates   646552..647427 (+) Length   291 a.a.
NCBI ID   WP_039099523.1    Uniprot ID   -
Organism   Latilactobacillus curvatus strain SRCM217410     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 641552..652427
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PGA57_RS03260 (PGA57_03260) - 641657..642484 (-) 828 WP_039099661.1 LysR family transcriptional regulator -
  PGA57_RS03265 (PGA57_03265) - 642593..643606 (+) 1014 WP_004265264.1 YeiH family protein -
  PGA57_RS03270 (PGA57_03270) - 643697..643936 (-) 240 WP_004270892.1 cytochrome b5 domain-containing protein -
  PGA57_RS03275 (PGA57_03275) - 644131..644466 (+) 336 WP_146954925.1 hypothetical protein -
  PGA57_RS03285 (PGA57_03285) - 644647..645024 (-) 378 WP_004270910.1 hypothetical protein -
  PGA57_RS03290 (PGA57_03290) - 645127..645627 (+) 501 WP_004270893.1 VanZ family protein -
  PGA57_RS03295 (PGA57_03295) - 645717..646448 (+) 732 WP_004270897.1 YebC/PmpR family DNA-binding transcriptional regulator -
  PGA57_RS03300 (PGA57_03300) comGA 646552..647427 (+) 876 WP_039099523.1 competence type IV pilus ATPase ComGA Machinery gene
  PGA57_RS03305 (PGA57_03305) comGB 647417..648418 (+) 1002 WP_146954924.1 type II secretion system F family protein Machinery gene
  PGA57_RS03310 (PGA57_03310) comGC 648415..648723 (+) 309 WP_004270901.1 competence type IV pilus major pilin ComGC Machinery gene
  PGA57_RS03315 (PGA57_03315) - 648788..649147 (+) 360 WP_158022155.1 hypothetical protein -
  PGA57_RS03320 (PGA57_03320) - 649134..649439 (+) 306 WP_039099485.1 hypothetical protein -
  PGA57_RS03325 (PGA57_03325) - 649387..649890 (+) 504 WP_158070645.1 competence type IV pilus minor pilin ComGF -
  PGA57_RS03330 (PGA57_03330) - 649868..650191 (+) 324 WP_221276460.1 hypothetical protein -
  PGA57_RS03335 (PGA57_03335) - 650307..651326 (+) 1020 WP_333780842.1 class I SAM-dependent methyltransferase -

Sequence

Protein


Download         Length: 291 a.a.        Molecular weight: 32042.84 Da        Isoelectric Point: 5.5654

>NTDB_id=775311 PGA57_RS03300 WP_039099523.1 646552..647427(+) (comGA) [Latilactobacillus curvatus strain SRCM217410]
MTEVLTTLLQKCATEKAQDLYFRPLSTGWAIIERLATGLKPLQKVTLSEGNQWLNQMKYQAGMDISETRRPQTGRSAIEV
AGELIYLRLATVGDFLNRESLVVRFIYPIGQTYHCDDSQILSRLMQLIQQPGLLLFAGPTGSGKTTSLYYLAEQVMQDKM
VVAIEDPIEIQQPAFLQLQVNEAAGLTYAALLKISLRLRPDTLIIGEIRDLQTAQHAVSAALSGHLVLSTIHARSTTGVM
DRLLDLGISHSQLTACLAGVAYQSLVVDGHNVAAHYELATAQQLEEAVHED

Nucleotide


Download         Length: 876 bp        

>NTDB_id=775311 PGA57_RS03300 WP_039099523.1 646552..647427(+) (comGA) [Latilactobacillus curvatus strain SRCM217410]
ATGACCGAAGTATTAACAACCCTTTTGCAAAAGTGCGCAACGGAAAAAGCGCAGGATCTTTATTTTCGACCACTATCGAC
GGGTTGGGCAATTATTGAACGCTTAGCGACAGGACTAAAGCCGTTACAAAAAGTTACTCTGTCAGAAGGCAATCAATGGC
TCAATCAAATGAAGTATCAAGCGGGGATGGATATCAGTGAAACTCGGCGTCCACAGACCGGCCGTTCAGCGATCGAGGTG
GCAGGCGAATTGATTTATTTACGGTTGGCAACCGTGGGGGATTTTTTGAATCGGGAAAGTTTAGTTGTTCGTTTCATTTA
TCCCATTGGGCAGACTTATCATTGCGACGACTCGCAGATTTTAAGTCGTTTAATGCAGTTAATTCAGCAACCAGGATTAT
TGCTATTTGCCGGTCCAACTGGTTCAGGGAAGACAACAAGTTTGTACTATCTTGCGGAACAAGTCATGCAAGATAAGATG
GTGGTTGCGATTGAAGATCCGATAGAGATTCAACAGCCAGCGTTCTTACAGTTACAAGTGAATGAAGCCGCAGGGTTAAC
CTATGCAGCATTGTTGAAAATCAGTTTGCGATTACGGCCTGATACCTTAATTATTGGTGAGATTCGCGACTTACAGACGG
CCCAACACGCGGTTTCTGCGGCGTTGAGCGGTCACTTGGTACTAAGTACGATTCACGCGCGAAGTACAACGGGGGTGATG
GACCGTCTATTAGATCTTGGAATTAGCCACTCACAATTAACCGCTTGTTTAGCGGGTGTTGCCTATCAATCACTAGTGGT
CGACGGCCACAATGTAGCGGCCCACTATGAGTTAGCAACTGCACAACAATTGGAGGAGGCTGTTCATGAAGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Latilactobacillus sakei subsp. sakei 23K

69.892

95.876

0.67
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

43.015

93.471

0.402
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

42.593

92.784

0.395
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

41.573

91.753

0.381
  comGA/cglA/cilD Streptococcus pneumoniae R6

41.573

91.753

0.381
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

41.573

91.753

0.381
  comGA/cglA/cilD Streptococcus pneumoniae D39

41.573

91.753

0.381
  comGA Lactococcus lactis subsp. cremoris KW2

41.603

90.034

0.375
  comYA Streptococcus mutans UA140

37.113

100

0.371
  comYA Streptococcus mutans UA159

37.113

100

0.371
  comYA Streptococcus gordonii str. Challis substr. CH1

39.194

93.814

0.368