Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PG878_RS07045 Genome accession   NZ_CP115873
Coordinates   1620909..1622039 (+) Length   376 a.a.
NCBI ID   WP_209029708.1    Uniprot ID   -
Organism   Xanthomonas hawaiiensis strain A6251     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1615909..1627039
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PG878_RS07020 (PG878_07010) - 1616177..1616629 (-) 453 WP_209029705.1 DUF4426 domain-containing protein -
  PG878_RS07025 (PG878_07015) proC 1616644..1617489 (-) 846 WP_209029706.1 pyrroline-5-carboxylate reductase -
  PG878_RS07030 (PG878_07020) - 1617619..1618647 (-) 1029 WP_209029870.1 hypothetical protein -
  PG878_RS07035 (PG878_07025) - 1618965..1619621 (-) 657 WP_244672465.1 YggS family pyridoxal phosphate-dependent enzyme -
  PG878_RS07040 (PG878_07030) pilT 1619744..1620781 (+) 1038 WP_010344138.1 type IV pilus twitching motility protein PilT Machinery gene
  PG878_RS07045 (PG878_07035) pilU 1620909..1622039 (+) 1131 WP_209029708.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PG878_RS07050 (PG878_07040) - 1622189..1623469 (+) 1281 WP_209029709.1 WD40 repeat domain-containing protein -
  PG878_RS07055 (PG878_07045) - 1623466..1624965 (+) 1500 WP_209029710.1 diguanylate cyclase -
  PG878_RS07060 (PG878_07050) - 1625673..1626272 (+) 600 WP_209029711.1 MFS transporter permease -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41922.19 Da        Isoelectric Point: 6.8811

>NTDB_id=774115 PG878_RS07045 WP_209029708.1 1620909..1622039(+) (pilU) [Xanthomonas hawaiiensis strain A6251]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKITPITQTPLTPQQSRDLVLNVMTPSQREEFEKTHECNFAIGVAG
VGRFRVSCFYQRNQVGMVLRRIETRIPSVDELNLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAISFAETGHLVLCTLHANNANQAM
DRVINFFPEDRRSQLLMDLSLNLRGVVAQQLIPTPDGKGRRVAMEIMLGTPLVQDYIREGEIHKLKDVMKESTNLGMKTF
DQSLFELYQAGEISYEDALRHADSQNEVRLRIKLAQGGDARTLAQGLDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=774115 PG878_RS07045 WP_209029708.1 1620909..1622039(+) (pilU) [Xanthomonas hawaiiensis strain A6251]
ATGAGCACCATCGACTTCACCTCTTTCCTGAAACTGATGGCGCACCAGAAGGCGTCGGACCTGTTCATCACCTCGGGCAT
GCCGCCGGCGATCAAGGTGCACGGCAAGATCACCCCGATCACGCAGACGCCGCTGACGCCGCAGCAGAGCCGCGACCTGG
TGCTGAACGTGATGACGCCGTCGCAGCGCGAGGAGTTCGAGAAGACCCACGAGTGCAACTTCGCCATCGGCGTGGCCGGG
GTCGGGCGCTTCCGCGTCAGTTGCTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCGCCGGATCGAGACGCGCATTCC
CAGCGTGGACGAACTCAACCTGCCGCCGGTGATCAAGACCCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGCACCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAACTCCACCGGGCACATCATCACC
ATCGAGGACCCGATCGAGTTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAGGTCGGCATCGACACCGACAG
CTGGGAGAACGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAGGGCA
TGGACCACGCCATCTCCTTTGCCGAGACCGGCCACCTGGTGCTGTGCACCCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCGTGATCAACTTCTTCCCCGAAGACCGCCGCAGCCAGTTGCTGATGGATCTGTCGCTGAACCTGCGCGGCGTGGT
CGCCCAGCAGCTGATCCCGACCCCGGACGGCAAGGGCCGCCGCGTGGCCATGGAGATCATGCTCGGCACGCCGCTGGTGC
AGGACTACATCCGCGAGGGCGAGATCCACAAGCTCAAGGACGTGATGAAGGAGTCCACCAACCTGGGCATGAAGACCTTC
GACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGAGGATGCGCTGCGCCACGCCGATTCGCAGAACGA
GGTGCGCCTGCGCATCAAGCTGGCCCAGGGCGGCGACGCACGCACCCTGGCGCAGGGCCTGGACGGGGTCGAGATCGCCG
AGGTGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.485

97.606

0.649
  pilU Acinetobacter baylyi ADP1

63.585

94.947

0.604
  pilU Vibrio cholerae strain A1552

52.809

94.681

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42

93.085

0.391
  pilT Pseudomonas aeruginosa PAK

41.003

90.16

0.37
  pilT Acinetobacter baumannii strain A118

40.407

91.489

0.37
  pilT Acinetobacter nosocomialis M2

40.407

91.489

0.37
  pilT Acinetobacter baumannii D1279779

40.407

91.489

0.37
  pilT Legionella pneumophila strain ERS1305867

40.597

89.096

0.362
  pilT Legionella pneumophila strain Lp02

40.597

89.096

0.362