Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   H7K58_RS02530 Genome accession   NZ_AP022188
Coordinates   532233..533474 (-) Length   413 a.a.
NCBI ID   WP_182787461.1    Uniprot ID   -
Organism   Aeromonas media strain WP7-W18-ESBL-02     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 527233..538474
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7K58_RS02505 (WP7W18E02_04850) mutT 529203..529607 (+) 405 WP_005332118.1 8-oxo-dGTP diphosphatase MutT -
  H7K58_RS02510 (WP7W18E02_04860) yacG 529683..529877 (-) 195 WP_042649030.1 DNA gyrase inhibitor YacG -
  H7K58_RS02515 (WP7W18E02_04870) zapD 529887..530609 (-) 723 WP_182787455.1 cell division protein ZapD -
  H7K58_RS02520 (WP7W18E02_04880) coaE 530647..531261 (-) 615 WP_182787457.1 dephospho-CoA kinase -
  H7K58_RS02525 (WP7W18E02_04890) pilD 531280..532152 (-) 873 WP_182787459.1 prepilin peptidase Machinery gene
  H7K58_RS02530 (WP7W18E02_04900) pilC 532233..533474 (-) 1242 WP_182787461.1 type II secretion system F family protein Machinery gene
  H7K58_RS02535 (WP7W18E02_04910) pilB 533604..535310 (-) 1707 WP_182787463.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  H7K58_RS02540 tapA 535314..535751 (-) 438 WP_182788811.1 type IVa pilus major pilin TapA -
  H7K58_RS02545 (WP7W18E02_04920) nadC 536076..536939 (-) 864 WP_182787465.1 carboxylating nicotinate-nucleotide diphosphorylase -
  H7K58_RS02550 (WP7W18E02_04930) - 536943..537404 (-) 462 WP_371316622.1 TIGR02281 family clan AA aspartic protease -
  H7K58_RS02555 (WP7W18E02_04940) ampD 537550..538122 (+) 573 WP_182787467.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 413 a.a.        Molecular weight: 45505.58 Da        Isoelectric Point: 10.1464

>NTDB_id=76998 H7K58_RS02530 WP_182787461.1 532233..533474(-) (pilC) [Aeromonas media strain WP7-W18-ESBL-02]
MATLAQKRNTPKKVFSFRWHGFNRKGQKVSGEFQADSINTVKAELRKQGVNVTKVSKQGQGLFSKGGARIKPMDIAVISR
QITTMLSAGVPLVQSLQIIARGHEKAAVRELIGQIATDVETGTPLSEALRRHPRHFDALYCDLVDAGEQSGALETIYDRI
ATYREKSEALKSKIKKAMFYPAMVILVAIIVTSILLLFVIPQFEEIFKSFGAELPVFTRFVIGISRFMQAWWYIIFGGAA
LAIFLYVRAWRKSQKVRDNTDRFILTIPVVGNILHKAAMARFARTLSTTFSAGIPLVDALVSAAGASGNYVYRTAILAIR
NEVVAGMQINVAMRTVDLFPDMVIQMVMIGEESGAIDDMLSKVAAIFEQEVDDMVDGLTSLLEPIIMVVLGVLVGGMVVA
MYLPIFKLGSVIH

Nucleotide


Download         Length: 1242 bp        

>NTDB_id=76998 H7K58_RS02530 WP_182787461.1 532233..533474(-) (pilC) [Aeromonas media strain WP7-W18-ESBL-02]
ATGGCAACATTAGCGCAAAAACGTAACACACCCAAAAAGGTCTTCTCCTTCCGCTGGCATGGATTCAATCGCAAGGGACA
AAAGGTTTCCGGTGAGTTCCAGGCCGACAGCATCAACACCGTCAAGGCGGAACTGCGCAAGCAGGGCGTCAATGTCACCA
AGGTCAGCAAGCAGGGCCAGGGCCTGTTTTCCAAGGGTGGTGCCCGGATCAAACCGATGGACATCGCCGTCATCTCCCGC
CAGATCACCACCATGCTCTCGGCCGGTGTGCCGCTGGTGCAAAGCCTGCAGATCATCGCCCGCGGTCATGAGAAGGCAGC
AGTACGCGAACTCATCGGCCAGATCGCCACCGATGTGGAAACCGGCACCCCGCTGTCAGAGGCGCTGCGCCGCCACCCCC
GCCACTTCGATGCGCTTTACTGCGACCTGGTAGACGCGGGGGAGCAGTCTGGCGCGCTGGAAACCATCTACGACCGGATT
GCCACATACCGCGAGAAGAGTGAGGCGCTGAAGTCCAAGATCAAGAAGGCCATGTTCTATCCCGCCATGGTCATCCTGGT
CGCCATCATCGTAACCTCCATCCTGCTGCTGTTCGTCATTCCCCAGTTCGAGGAGATCTTCAAGAGCTTCGGAGCAGAGC
TGCCAGTCTTCACCCGATTCGTCATCGGCATCTCCCGCTTCATGCAAGCTTGGTGGTATATCATTTTTGGTGGCGCAGCC
TTGGCCATCTTCCTCTATGTGCGCGCCTGGCGAAAATCCCAGAAGGTACGGGACAACACGGACAGATTCATCCTCACCAT
TCCGGTCGTGGGCAACATATTGCACAAGGCGGCCATGGCCCGTTTCGCCCGTACCCTCTCCACCACCTTCTCCGCCGGGA
TACCTCTGGTGGATGCCTTGGTGTCAGCGGCCGGCGCCTCTGGCAACTATGTCTATCGCACGGCCATCCTGGCCATTCGC
AACGAGGTGGTGGCCGGCATGCAGATCAACGTCGCCATGCGTACCGTGGATCTCTTCCCAGACATGGTGATCCAGATGGT
GATGATAGGTGAGGAGTCCGGTGCCATCGATGACATGCTCTCCAAGGTCGCCGCCATCTTCGAGCAGGAGGTGGACGACA
TGGTCGATGGCCTCACCAGCCTGCTGGAGCCCATCATCATGGTGGTACTCGGGGTGCTGGTCGGTGGCATGGTTGTCGCC
ATGTACCTCCCCATCTTCAAGCTGGGCTCTGTGATACACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(282-404)

Type II secretion system (T2SS), protein F (T2SSF)

(79-201)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

56.818

95.884

0.545
  pilC Legionella pneumophila strain ERS1305867

53.086

98.063

0.521
  pilC Acinetobacter baumannii D1279779

51.122

97.094

0.496
  pilC Acinetobacter baylyi ADP1

51.378

96.61

0.496
  pilC Vibrio cholerae strain A1552

48.25

96.852

0.467
  pilC Vibrio campbellii strain DS40M4

46.482

96.368

0.448
  pilG Neisseria gonorrhoeae MS11

40.05

97.337

0.39
  pilG Neisseria meningitidis 44/76-A

39.801

97.337

0.387
  pilC Thermus thermophilus HB27

39.25

96.852

0.38

Multiple sequence alignment