Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   H7K58_RS02535 Genome accession   NZ_AP022188
Coordinates   533604..535310 (-) Length   568 a.a.
NCBI ID   WP_182787463.1    Uniprot ID   -
Organism   Aeromonas media strain WP7-W18-ESBL-02     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 528604..540310
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7K58_RS02505 (WP7W18E02_04850) mutT 529203..529607 (+) 405 WP_005332118.1 8-oxo-dGTP diphosphatase MutT -
  H7K58_RS02510 (WP7W18E02_04860) yacG 529683..529877 (-) 195 WP_042649030.1 DNA gyrase inhibitor YacG -
  H7K58_RS02515 (WP7W18E02_04870) zapD 529887..530609 (-) 723 WP_182787455.1 cell division protein ZapD -
  H7K58_RS02520 (WP7W18E02_04880) coaE 530647..531261 (-) 615 WP_182787457.1 dephospho-CoA kinase -
  H7K58_RS02525 (WP7W18E02_04890) pilD 531280..532152 (-) 873 WP_182787459.1 prepilin peptidase Machinery gene
  H7K58_RS02530 (WP7W18E02_04900) pilC 532233..533474 (-) 1242 WP_182787461.1 type II secretion system F family protein Machinery gene
  H7K58_RS02535 (WP7W18E02_04910) pilB 533604..535310 (-) 1707 WP_182787463.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  H7K58_RS02540 tapA 535314..535751 (-) 438 WP_182788811.1 type IVa pilus major pilin TapA -
  H7K58_RS02545 (WP7W18E02_04920) nadC 536076..536939 (-) 864 WP_182787465.1 carboxylating nicotinate-nucleotide diphosphorylase -
  H7K58_RS02550 (WP7W18E02_04930) - 536943..537404 (-) 462 WP_371316622.1 TIGR02281 family clan AA aspartic protease -
  H7K58_RS02555 (WP7W18E02_04940) ampD 537550..538122 (+) 573 WP_182787467.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  H7K58_RS02560 (WP7W18E02_04950) pdhR 538399..539163 (+) 765 WP_005329211.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62680.07 Da        Isoelectric Point: 5.8224

>NTDB_id=76999 H7K58_RS02535 WP_182787463.1 533604..535310(-) (pilB) [Aeromonas media strain WP7-W18-ESBL-02]
MTSSPNSGLATSLAASSLLSEPDSQRYLSQARAQRKPFVTFLIENDILDSKALADFCELEYGVPLLDLAAFDLSEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEEGKLTAAIGKLMESEQDTLGMDHIDEAEI
SELEVSDENSRLDDSVNTADDDAPIVKYIHKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFAAR
LKVMARLDIAERRLPQDGRIKLKISRNRSMDMRVNTLPTMWGEKVVIRLLDSSVSRLNLDQLGFDERQKAQYLHALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTSEVNISTAEDPVEINLPGVNQVQINPKAGLTFASALRSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKVPEAVPEAELLEFGFT
QQQLAAGLRLFKPVGCKECSGGYRGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRISGLEKARLGVTSLA
EINRITTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=76999 H7K58_RS02535 WP_182787463.1 533604..535310(-) (pilB) [Aeromonas media strain WP7-W18-ESBL-02]
ATGACCTCTAGCCCCAATAGCGGCCTGGCCACCAGCCTGGCCGCTTCTTCCCTCCTCAGTGAGCCGGACTCACAGCGCTA
TCTGAGCCAGGCAAGGGCGCAGCGCAAGCCCTTTGTCACCTTCCTGATCGAAAACGACATCCTCGACAGCAAAGCTCTCG
CAGACTTTTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTCTCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAATTGATTGAGAAACACCACGTCCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
CCCGACCAATGTGTCGGCCCTGGAGGATTTCGGCTTCAGCTTCGGCCTGCACACCGAGGCCTTGCTGGTCGAAGAGGGCA
AGCTGACCGCCGCCATCGGCAAGCTGATGGAAAGCGAGCAGGATACCCTGGGCATGGATCACATCGATGAGGCCGAAATC
TCGGAACTCGAGGTCTCCGATGAGAATTCCCGCCTCGACGACAGCGTCAATACCGCCGACGACGATGCCCCCATCGTCAA
ATACATTCACAAGATCATGATGGACGCCATCAAGCGTGGCGCATCCGACCTGCATTTCGAACCCTATGAGACCAAGTACC
GGATCCGCTTCCGAATCGACGGCATACTGCACGAAATCGCCACCCCGCCGGTCAATCTGGCGAACCGTTTCGCGGCTCGC
TTAAAGGTAATGGCGCGGCTCGATATCGCCGAGCGGCGCCTGCCCCAGGATGGACGGATCAAGCTGAAGATCTCTCGCAA
CCGGTCCATGGATATGCGGGTCAACACCCTGCCCACCATGTGGGGAGAAAAGGTCGTCATTCGCCTGCTGGACTCGTCAG
TATCTCGCCTCAACCTCGACCAACTCGGCTTCGACGAACGCCAGAAGGCGCAATATCTGCATGCGCTCTCCAAGCCACAG
GGCATGATCCTGGTCACGGGCCCCACGGGATCGGGCAAGACAGTATCACTCTATACCGGTCTCAACATCCTCAATACCAG
CGAGGTCAACATCTCCACCGCCGAGGATCCGGTGGAGATCAACCTGCCCGGCGTCAACCAGGTGCAGATCAATCCCAAGG
CGGGGCTCACCTTCGCCAGTGCCCTGCGCTCCTTCCTGCGCCAGGATCCTGACATCGTCATGGTGGGGGAGATCCGGGAT
CTGGAAACCGCCGAGATTGCCATCAAGGCCGCTCAGACCGGACACCTGGTGCTGTCAACCCTGCACACCAACTCGGCGGC
CGAGACCCTGACCCGGATGATGAACATGGGGGTGCCCGCCTTCAACATCGCCTCCTCGGTGACCCTGATCATGGCCCAGC
GCCTCGCCCGCAAGCTGTGCGATCACTGCAAGGTGCCCGAGGCAGTGCCGGAAGCCGAATTGCTGGAGTTCGGTTTTACC
CAGCAACAGCTGGCCGCCGGTTTGCGGCTGTTCAAACCGGTCGGTTGCAAGGAGTGTTCGGGAGGATACAGAGGACGGGT
CGGCATCTATGAAATCATGCTGATGTCGGAGAACATCGCCAAGCTGATCATGCAGGGAGCAAACTCGCTGCAGATCGCTG
CCATCGCCCAGAAGGAGGGTATGCGTACCCTGCGGATTTCCGGCCTGGAGAAGGCCCGTCTGGGGGTCACCAGCCTGGCC
GAGATCAACAGGATCACGACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.85

99.824

0.548
  pilB Acinetobacter baylyi ADP1

54.211

100

0.544
  pilB Legionella pneumophila strain ERS1305867

53.901

99.296

0.535
  pilB Vibrio cholerae strain A1552

52.837

99.296

0.525
  pilB Vibrio parahaemolyticus RIMD 2210633

49.466

98.944

0.489
  pilB Vibrio campbellii strain DS40M4

49.378

99.12

0.489
  pilF Neisseria gonorrhoeae MS11

46.277

99.296

0.46
  pilF Thermus thermophilus HB27

39.736

93.486

0.371
  pilB/pilB1 Synechocystis sp. PCC 6803

38.043

97.183

0.37

Multiple sequence alignment