Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   OSH14_RS16065 Genome accession   NZ_CP113071
Coordinates   3420296..3420745 (-) Length   149 a.a.
NCBI ID   WP_000993725.1    Uniprot ID   A0A9P2QEL2
Organism   Acinetobacter baumannii strain A118 DeltaH0N27_10830::kan     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3415296..3425745
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OSH14_RS16045 (OSH14_16045) - 3416949..3417332 (+) 384 WP_000090000.1 RidA family protein -
  OSH14_RS16050 (OSH14_16050) - 3417542..3417736 (+) 195 WP_000289091.1 bacterioferritin-associated ferredoxin -
  OSH14_RS16055 (OSH14_16055) bfr 3417981..3418445 (+) 465 WP_000678123.1 bacterioferritin -
  OSH14_RS16060 (OSH14_16060) - 3418485..3420113 (-) 1629 WP_168726695.1 O-antigen ligase family protein -
  OSH14_RS16065 (OSH14_16065) pilA 3420296..3420745 (-) 450 WP_000993725.1 pilin Machinery gene
  OSH14_RS16070 (OSH14_16070) - 3421089..3421649 (-) 561 WP_000258949.1 TPM domain-containing protein -
  OSH14_RS16075 (OSH14_16075) - 3421643..3422725 (-) 1083 WP_168726694.1 TPM domain-containing protein -
  OSH14_RS16080 (OSH14_16080) - 3422747..3423337 (-) 591 WP_000846931.1 LemA family protein -
  OSH14_RS16085 (OSH14_16085) - 3423468..3424355 (-) 888 WP_001017319.1 metal-dependent hydrolase -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15475.66 Da        Isoelectric Point: 4.5701

>NTDB_id=759791 OSH14_RS16065 WP_000993725.1 3420296..3420745(-) (pilA) [Acinetobacter baumannii strain A118 DeltaH0N27_10830::kan]
MNAQKGFTLIELMIVVAIIGILAAIAIPQYQNYIAKSQVNSGFSEISSIKTGYENAVNEGAVPTKLSDVGFTAATSSVCA
TYSIDAFTETDGAGKITCTLKGNPKIATKIVELTRSTDGAWTCVSDVPTEFLPKGCTAGTPTEGAITSL

Nucleotide


Download         Length: 450 bp        

>NTDB_id=759791 OSH14_RS16065 WP_000993725.1 3420296..3420745(-) (pilA) [Acinetobacter baumannii strain A118 DeltaH0N27_10830::kan]
ATGAATGCACAAAAAGGTTTTACATTAATCGAACTCATGATCGTGGTTGCTATTATCGGTATTTTGGCAGCAATTGCGAT
TCCGCAATATCAAAACTATATCGCTAAATCACAAGTGAACAGTGGATTTTCTGAAATCAGTTCTATTAAAACTGGATATG
AAAATGCTGTAAATGAAGGAGCAGTTCCTACCAAGTTATCTGATGTTGGTTTTACTGCAGCTACCTCTAGTGTTTGTGCT
ACATATAGTATTGACGCATTTACTGAAACAGATGGAGCAGGTAAGATTACTTGTACTCTTAAAGGTAATCCAAAAATTGC
TACTAAGATAGTTGAATTAACTCGTTCTACAGATGGTGCTTGGACTTGTGTTTCTGATGTGCCGACAGAGTTTTTACCAA
AAGGTTGTACCGCAGGTACACCAACAGAAGGTGCTATCACTAGTTTGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

100

100

1
  pilA Pseudomonas aeruginosa PAK

45.333

100

0.456
  pilA Vibrio cholerae strain A1552

42.667

100

0.43
  pilA Vibrio cholerae C6706

42.667

100

0.43
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

42.667

100

0.43
  pilA/pilAI Pseudomonas stutzeri DSM 10701

45.985

91.946

0.423
  pilA/pilAII Pseudomonas stutzeri DSM 10701

43.796

91.946

0.403
  comP Acinetobacter baylyi ADP1

40

97.315

0.389
  pilA2 Legionella pneumophila str. Paris

39.726

97.987

0.389
  pilA2 Legionella pneumophila strain ERS1305867

39.041

97.987

0.383