Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PHLH3_RS05580 Genome accession   NZ_AP021905
Coordinates   1170396..1171613 (-) Length   405 a.a.
NCBI ID   WP_213628507.1    Uniprot ID   -
Organism   Pseudomonas sp. Pc102     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1165396..1176613
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PHLH3_RS05535 (PHLH8_10980) - 1165818..1165991 (-) 174 WP_173173318.1 DUF3094 domain-containing protein -
  PHLH3_RS05540 (PHLH8_10990) - 1166078..1166704 (-) 627 WP_173173316.1 DUF1780 domain-containing protein -
  PHLH3_RS05545 (PHLH8_11000) - 1166816..1167241 (+) 426 WP_213628502.1 GNAT family N-acetyltransferase -
  PHLH3_RS05550 (PHLH8_11010) - 1167238..1167705 (+) 468 WP_111263619.1 FAD/FMN-containing dehydrogenase -
  PHLH3_RS05555 (PHLH8_11020) - 1167702..1168388 (+) 687 WP_213628503.1 energy-coupling factor ABC transporter permease -
  PHLH3_RS05560 (PHLH8_11030) - 1168496..1168711 (+) 216 WP_213628504.1 hypothetical protein -
  PHLH3_RS05565 (PHLH8_11040) yacG 1168715..1168915 (-) 201 WP_111263622.1 DNA gyrase inhibitor YacG -
  PHLH3_RS05570 (PHLH8_11050) coaE 1168912..1169523 (-) 612 WP_213628505.1 dephospho-CoA kinase -
  PHLH3_RS05575 (PHLH8_11060) pilD 1169523..1170392 (-) 870 WP_213628506.1 A24 family peptidase Machinery gene
  PHLH3_RS05580 (PHLH8_11070) pilC 1170396..1171613 (-) 1218 WP_213628507.1 type II secretion system F family protein Machinery gene
  PHLH3_RS05585 (PHLH8_11080) pilB 1171617..1173320 (-) 1704 WP_213628508.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PHLH3_RS05590 - 1173547..1173972 (+) 426 WP_213628509.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PHLH3_RS05595 (PHLH8_11090) - 1174034..1175647 (+) 1614 WP_213628510.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44380.22 Da        Isoelectric Point: 9.4778

>NTDB_id=75748 PHLH3_RS05580 WP_213628507.1 1170396..1171613(-) (pilC) [Pseudomonas sp. Pc102]
MAEKALKTSLFKWEGTDRKGTKVTGEISGTNPALVKAQLRKQGINPTKVRKKPVSLFSGGKKIKPLDIALFTRQMATMMK
AGVPLLQSFDIIGEGFDNPNMRKLVDDVKQDVAAGNSFATSLRKRPEYFDDLYCNLVDSGEQAGALETLLDRVATYKEKT
EALKAKIKKAMNYPIAVVLIAVIVSAILLIKVVPQFQSIFENFGAQLPAFTLMVIWMSEVLQEWWFIVVIVLFCAIYGLK
QAHQRSEAFRDWIDKAVLKIPVVGDILYKSAVARYARTLATTFAAGVPLVDALDSVSGATGNVVFKRAVNKIKQDVSTGM
QLNFSMRTTGVFPTMAIQMTAIGEESGALDTMLDKVASFYEEEVDNAVDGLTALMEPMIMAVLGVLIGGLIIAMYLPIFQ
LGSVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=75748 PHLH3_RS05580 WP_213628507.1 1170396..1171613(-) (pilC) [Pseudomonas sp. Pc102]
ATGGCGGAAAAAGCGTTAAAAACAAGTCTTTTCAAATGGGAAGGCACCGATCGAAAAGGCACCAAGGTCACCGGCGAAAT
TTCTGGCACCAACCCTGCCTTGGTCAAGGCCCAGCTTCGCAAGCAGGGCATCAACCCCACCAAAGTCCGTAAAAAGCCAG
TATCGCTCTTCAGTGGCGGCAAGAAGATCAAGCCTCTCGACATCGCGCTTTTCACGCGCCAGATGGCGACGATGATGAAA
GCGGGTGTGCCCCTGCTACAGAGCTTCGACATCATCGGCGAGGGCTTTGATAACCCGAACATGCGCAAGCTAGTCGATGA
TGTCAAACAGGACGTCGCGGCTGGTAACAGCTTTGCCACTTCTCTAAGAAAGCGCCCCGAGTACTTTGATGACCTGTACT
GCAACCTGGTGGACTCGGGTGAGCAAGCAGGCGCACTCGAGACATTGCTAGACCGTGTAGCAACCTACAAGGAAAAAACC
GAGGCCCTGAAAGCCAAGATAAAGAAGGCGATGAACTATCCCATTGCCGTTGTACTTATCGCAGTCATCGTTTCCGCCAT
CCTGCTGATCAAGGTGGTGCCGCAGTTCCAGTCGATCTTTGAAAACTTCGGCGCACAGCTACCCGCATTCACCTTGATGG
TCATCTGGATGTCCGAAGTACTGCAGGAATGGTGGTTTATCGTTGTAATAGTGCTTTTCTGTGCAATCTATGGACTCAAG
CAAGCCCACCAGAGGTCAGAAGCATTCCGTGACTGGATAGACAAAGCCGTCTTGAAGATTCCGGTAGTAGGCGACATCCT
CTATAAATCCGCCGTAGCCCGTTATGCACGAACCCTCGCAACCACCTTCGCTGCGGGTGTACCGCTTGTCGACGCCCTGG
ACTCCGTTTCAGGAGCAACCGGTAACGTAGTATTCAAACGCGCAGTGAATAAGATCAAACAAGACGTTTCCACCGGTATG
CAACTGAACTTTTCGATGCGTACTACTGGCGTATTCCCGACCATGGCCATCCAGATGACAGCCATCGGTGAGGAATCCGG
TGCACTGGACACCATGCTCGACAAGGTTGCCTCCTTCTACGAAGAAGAGGTCGACAACGCCGTTGATGGCCTGACAGCCC
TGATGGAACCGATGATCATGGCGGTTCTAGGGGTACTGATCGGCGGCCTGATCATCGCCATGTACCTGCCCATCTTCCAG
CTCGGTTCCGTGGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

81.975

100

0.82
  pilC Acinetobacter baylyi ADP1

60.591

100

0.607
  pilC Acinetobacter baumannii D1279779

59.753

100

0.598
  pilC Legionella pneumophila strain ERS1305867

53.535

97.778

0.523
  pilG Neisseria gonorrhoeae MS11

44.03

99.259

0.437
  pilG Neisseria meningitidis 44/76-A

43.781

99.259

0.435
  pilC Vibrio cholerae strain A1552

40.741

100

0.407
  pilC Vibrio campbellii strain DS40M4

40.152

97.778

0.393
  pilC Thermus thermophilus HB27

37

98.765

0.365

Multiple sequence alignment