Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PHLH3_RS05585 Genome accession   NZ_AP021905
Coordinates   1171617..1173320 (-) Length   567 a.a.
NCBI ID   WP_213628508.1    Uniprot ID   -
Organism   Pseudomonas sp. Pc102     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1166617..1178320
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PHLH3_RS05545 (PHLH8_11000) - 1166816..1167241 (+) 426 WP_213628502.1 GNAT family N-acetyltransferase -
  PHLH3_RS05550 (PHLH8_11010) - 1167238..1167705 (+) 468 WP_111263619.1 FAD/FMN-containing dehydrogenase -
  PHLH3_RS05555 (PHLH8_11020) - 1167702..1168388 (+) 687 WP_213628503.1 energy-coupling factor ABC transporter permease -
  PHLH3_RS05560 (PHLH8_11030) - 1168496..1168711 (+) 216 WP_213628504.1 hypothetical protein -
  PHLH3_RS05565 (PHLH8_11040) yacG 1168715..1168915 (-) 201 WP_111263622.1 DNA gyrase inhibitor YacG -
  PHLH3_RS05570 (PHLH8_11050) coaE 1168912..1169523 (-) 612 WP_213628505.1 dephospho-CoA kinase -
  PHLH3_RS05575 (PHLH8_11060) pilD 1169523..1170392 (-) 870 WP_213628506.1 A24 family peptidase Machinery gene
  PHLH3_RS05580 (PHLH8_11070) pilC 1170396..1171613 (-) 1218 WP_213628507.1 type II secretion system F family protein Machinery gene
  PHLH3_RS05585 (PHLH8_11080) pilB 1171617..1173320 (-) 1704 WP_213628508.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PHLH3_RS05590 - 1173547..1173972 (+) 426 WP_213628509.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PHLH3_RS05595 (PHLH8_11090) - 1174034..1175647 (+) 1614 WP_213628510.1 tetratricopeptide repeat protein -
  PHLH3_RS05600 (PHLH8_11100) - 1175640..1177400 (+) 1761 WP_213628511.1 Wzy polymerase domain-containing protein -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62439.50 Da        Isoelectric Point: 5.4665

>NTDB_id=75749 PHLH3_RS05585 WP_213628508.1 1171617..1173320(-) (pilB) [Pseudomonas sp. Pc102]
MTDTPTLTGLARQLVAANLMDERAAQQAFVQAQRNKISLVTYLAQNKLLKSRDLAELAAEQFGIAFLDLNSVDKEGQPKE
LVTEKLIRQHRALPLWRRGNKLFIAVSDPTNHQATTDIQFSTGLTTEAILVEDDKLGDAIDKFFDSATTTFDDLGDVDLE
GLDVEAVDDSKQDAATGQDADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKSYRVRLRTDGMLHEVARPPIQLASRISAR
LKVMAALDISERRKPQDGRIKMKISKSKSIDFRVNTLPTLWGEKIVMRILDPSSAQMGIDALGYEDFQKDLYMTALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGMDFAQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCSACKKEVDVPRDTLIKEGFP
ENQVGSFKIFGPVGCESCKGGYKGRVGIYEVVKITPALQRIIMEEGNSIDIAAQMRKDGFNDLRTSGLLKAMQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=75749 PHLH3_RS05585 WP_213628508.1 1171617..1173320(-) (pilB) [Pseudomonas sp. Pc102]
ATGACGGATACTCCCACTCTTACCGGCCTCGCACGTCAATTGGTTGCGGCCAACCTGATGGATGAGCGCGCTGCCCAGCA
AGCATTCGTACAGGCGCAGCGAAACAAGATTTCTCTGGTGACCTACCTCGCGCAGAACAAGCTGCTGAAAAGCCGGGACC
TTGCCGAGCTGGCAGCCGAGCAGTTCGGCATCGCCTTTCTCGACCTCAATAGCGTCGACAAGGAGGGGCAGCCCAAGGAA
CTGGTCACCGAGAAGCTGATTCGCCAGCACAGGGCACTTCCGCTCTGGCGTCGAGGCAACAAGCTATTCATTGCGGTATC
GGACCCGACCAACCACCAGGCCACTACCGATATCCAGTTCAGCACGGGGCTCACCACCGAGGCGATTCTGGTCGAAGATG
ACAAGCTTGGCGATGCCATCGACAAGTTCTTCGACAGCGCTACCACCACCTTCGATGATCTGGGTGATGTGGATCTTGAA
GGGCTGGACGTCGAGGCCGTAGATGACAGCAAGCAGGATGCTGCTACAGGTCAGGACGCCGATGACGCACCCGTTGTGCG
TTTCGTCAACAAGATGCTGCTGGATGCCATCAAGGGTGGCTCGTCCGACCTGCATTTCGAGCCCTACGAGAAGTCCTACC
GGGTACGCTTGCGCACAGACGGCATGTTGCATGAAGTAGCCCGCCCTCCGATTCAACTGGCCAGCCGCATCTCAGCTCGC
CTGAAGGTCATGGCGGCACTCGACATATCCGAGCGCCGCAAACCCCAGGACGGGCGTATCAAGATGAAGATCTCGAAGTC
GAAATCCATTGATTTCCGTGTCAACACGCTGCCAACCCTCTGGGGCGAGAAGATCGTGATGCGTATTCTCGACCCATCCA
GCGCACAGATGGGTATTGATGCGCTGGGCTACGAGGACTTCCAGAAAGATCTCTACATGACAGCCCTGAAGCAGCCACAG
GGCATGATCCTGGTAACGGGACCTACAGGTTCGGGAAAGACCGTATCCCTCTACACCGGTCTCAATATCCTCAACACAGT
GGATATCAATATCTCCACGGCTGAAGACCCGGTAGAGATCAACCTGGAGGGCATCAACCAGGTCAACGTTAATCCCCGTC
AAGGTATGGACTTCGCGCAAGCACTGCGCGCCTTTCTTCGGCAGGACCCCGATGTGATCATGGTTGGCGAGATCCGCGAC
CTTGAAACAGCCGAGATTGCCATCAAGGCTGCACAAACCGGCCATATGGTGATGTCCACACTTCACACCAACAGCGCTGC
CGAAACCCTGACCCGCCTGCGAAACATGGGCGTACCCGCATTCAACATTGCGACCTCAGTCAATCTGATCATCGCGCAAC
GACTGGCACGCAAGTTGTGCAGCGCCTGCAAGAAGGAAGTCGATGTACCTCGAGACACCCTGATCAAGGAAGGCTTCCCA
GAAAACCAGGTTGGCTCCTTCAAGATTTTCGGGCCTGTAGGCTGTGAAAGCTGCAAGGGCGGCTACAAAGGCAGGGTAGG
TATTTATGAAGTAGTTAAAATCACGCCTGCCCTGCAGCGGATTATCATGGAAGAAGGCAACTCCATCGATATTGCTGCGC
AGATGCGCAAAGATGGGTTCAACGATCTGCGCACCTCGGGCCTGCTTAAGGCCATGCAAGGCGTGACGAGTCTCGAGGAA
GTCAACCGCGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-148)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

60.035

100

0.601
  pilB Acinetobacter baylyi ADP1

59.474

100

0.598
  pilB Legionella pneumophila strain ERS1305867

55.846

100

0.564
  pilB Vibrio cholerae strain A1552

50

99.118

0.496
  pilF Neisseria gonorrhoeae MS11

49.645

99.471

0.494
  pilB Vibrio campbellii strain DS40M4

48.596

100

0.489
  pilB Vibrio parahaemolyticus RIMD 2210633

50.183

96.296

0.483
  pilB/pilB1 Synechocystis sp. PCC 6803

35.795

100

0.393
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.13

93.298

0.365

Multiple sequence alignment