Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   OOT43_RS20155 Genome accession   NZ_CP110921
Coordinates   4289384..4290598 (+) Length   404 a.a.
NCBI ID   WP_266022504.1    Uniprot ID   -
Organism   Methylococcus mesophilus strain 16-5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4284384..4295598
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OOT43_RS20145 (OOT43_20145) - 4284849..4287614 (+) 2766 WP_266022502.1 valine--tRNA ligase -
  OOT43_RS20150 (OOT43_20150) pilB 4287664..4289379 (+) 1716 WP_266022503.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OOT43_RS20155 (OOT43_20155) pilC 4289384..4290598 (+) 1215 WP_266022504.1 type II secretion system F family protein Machinery gene
  OOT43_RS20160 (OOT43_20160) pilD 4290609..4291487 (+) 879 WP_266022505.1 A24 family peptidase Machinery gene
  OOT43_RS20165 (OOT43_20165) coaE 4291495..4292091 (+) 597 WP_266022506.1 dephospho-CoA kinase -
  OOT43_RS20170 (OOT43_20170) zapD 4292193..4292960 (+) 768 WP_266022507.1 cell division protein ZapD -
  OOT43_RS20175 (OOT43_20175) yacG 4292972..4293187 (+) 216 WP_266022508.1 DNA gyrase inhibitor YacG -
  OOT43_RS20180 (OOT43_20180) cysG 4293226..4294626 (-) 1401 WP_266022509.1 siroheme synthase CysG -
  OOT43_RS20185 (OOT43_20185) - 4294674..4294904 (+) 231 WP_266022510.1 sulfurtransferase TusA family protein -
  OOT43_RS20190 (OOT43_20190) - 4294912..4295574 (-) 663 WP_266022511.1 HAD family phosphatase -

Sequence

Protein


Download         Length: 404 a.a.        Molecular weight: 43951.57 Da        Isoelectric Point: 9.6644

>NTDB_id=756950 OOT43_RS20155 WP_266022504.1 4289384..4290598(+) (pilC) [Methylococcus mesophilus strain 16-5]
MASQETVLFVWEGLDRNGSRTKGEVSARSEITARAELRRQGIRVVKIKRKPKPLFGGPRKRITPKHIAVFSRQLATMLSA
GVPLVQAFEIVGRGHDNPAMQDLLLGIKADVESGTTLADALSKHPVHFDELFCNLVRAGEQAGVLETLLHKVADYKEKTE
SLKGKIKKALTYPIAVIVVAVIVTSILLIFVVPTFEDLFKSFGADLPAFTQLVIDLSRWLRDWWYVAFGSLGVAATAFVK
ARQRSPAFNHLLDRLALGVPVVGAILHKAAVARFARTLSTMSAAGVPLVEALQSVAGATGNIIYGEAVMRMREDVSIGQQ
LQMSMRQANLFPNMVMQMVAIGEESGSLDSMLSKVADFYEEEVDNAVDSLSSLLEPLIMVVLGVLVGGLVIAMYLPIFKL
GSVV

Nucleotide


Download         Length: 1215 bp        

>NTDB_id=756950 OOT43_RS20155 WP_266022504.1 4289384..4290598(+) (pilC) [Methylococcus mesophilus strain 16-5]
GTGGCGAGCCAGGAAACCGTATTGTTCGTCTGGGAGGGCCTGGACAGGAACGGCAGCCGGACCAAGGGCGAAGTCAGCGC
CCGGTCGGAGATCACCGCGCGCGCGGAGCTGAGACGGCAGGGCATCCGCGTCGTCAAGATCAAAAGGAAGCCCAAGCCGC
TGTTCGGCGGTCCGCGCAAGAGGATCACGCCGAAGCACATCGCGGTGTTCAGCCGCCAACTCGCGACCATGCTGTCGGCC
GGGGTGCCCCTGGTGCAGGCTTTCGAAATCGTGGGCCGCGGCCACGACAATCCGGCCATGCAGGATCTCCTCCTGGGGAT
CAAGGCAGACGTCGAGAGCGGCACGACCCTGGCGGATGCGCTGTCGAAGCACCCGGTCCATTTCGACGAGCTGTTCTGCA
ACTTGGTCAGGGCCGGCGAACAGGCCGGTGTGCTGGAAACCCTGCTGCACAAGGTGGCCGATTACAAGGAAAAAACCGAG
TCCCTCAAGGGCAAGATCAAGAAGGCTTTGACCTATCCGATCGCGGTGATCGTCGTTGCGGTGATCGTGACTTCCATCCT
GCTGATTTTCGTGGTGCCGACCTTCGAGGACCTGTTCAAGAGCTTCGGTGCCGACCTGCCCGCCTTCACCCAACTGGTGA
TCGATCTTTCGCGCTGGCTGCGTGACTGGTGGTACGTGGCCTTCGGCTCGCTGGGCGTCGCGGCGACGGCGTTCGTGAAG
GCCCGGCAACGTTCGCCGGCGTTCAATCATCTGCTGGACCGGCTGGCCCTGGGAGTGCCGGTTGTCGGCGCCATTCTGCA
CAAGGCCGCCGTCGCCCGCTTCGCCAGGACCTTGTCCACCATGTCCGCGGCAGGCGTACCCTTGGTCGAGGCGCTGCAGT
CGGTGGCGGGCGCGACCGGAAACATCATTTACGGCGAGGCGGTCATGCGTATGCGCGAAGACGTATCCATCGGCCAGCAG
CTCCAGATGTCGATGCGCCAGGCCAACCTGTTCCCCAACATGGTGATGCAGATGGTGGCGATCGGCGAGGAATCGGGGTC
GCTCGACAGCATGCTGTCGAAAGTCGCCGATTTCTACGAGGAAGAAGTGGACAATGCCGTGGACTCGCTGAGCAGCCTGC
TCGAGCCTTTGATCATGGTGGTTCTGGGTGTGCTGGTCGGGGGGCTCGTGATCGCCATGTATCTGCCGATTTTCAAACTG
GGTTCCGTCGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(70-193)

Type II secretion system (T2SS), protein F (T2SSF)

(274-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Legionella pneumophila strain ERS1305867

60.606

98.02

0.594
  pilC Pseudomonas stutzeri DSM 10701

59.95

98.267

0.589
  pilC Acinetobacter baylyi ADP1

57.843

100

0.584
  pilC Acinetobacter baumannii D1279779

58.69

98.267

0.577
  pilG Neisseria meningitidis 44/76-A

47.859

98.267

0.47
  pilG Neisseria gonorrhoeae MS11

47.859

98.267

0.47
  pilC Vibrio campbellii strain DS40M4

43.86

98.762

0.433
  pilC Vibrio cholerae strain A1552

43.687

98.02

0.428
  pilC Thermus thermophilus HB27

40.852

98.762

0.403